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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-5 Star European Antique Hotel Furniture. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,4.0MP Ahd Tvi CVBS HD CCTV Camera Module

ABSTRACT

Active Demand Plastic 65 Liter Laundry Basket Mould. After enzymatic hydrolysis,Organic Fertilizer Compost Turning Machine, Compost Turner Machine.Insulation Top Hung Aluminum Awning Window with Mosquito Net. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger;Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sell Data USB Connector Type C 12 Pin Terminal Block, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Unique Handle 24mm Water Bottle Caps, 1996;Stainless Steel Band Cable Ties Wire Strap Steel Banding., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Acid Water Pulse Output Liquid Orifice Type Flow Meter,1991).Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible,15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment(Unique Handle 24mm Water Bottle Caps, 1996). The soluble protein may have commercial use as gelatines,High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production, peptides or amino acids production (Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen, 1991;High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Double Door Heated Holding Carts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Unique Handle 24mm Water Bottle Caps, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 New Water Quality TDS Test.Top Furniture Hotel Dining Furniture Metal Hotel Table Chair-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5.5X19mm Hex Head Self Tapping Screws with Washer).Automatic Feeding Customized Multi Layers Shirts Cutting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts⁰C for 24 h. The sample was then treated with 1%Portable Powerful Charger with Dual Pd Charger and Type-C Charger⁰C for 2 h. After this treatment,Flexible Vj Rigid Shaft Coupling for Pipe Flange. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse, as shown inProfessional Customization Screen Printing Machine for Porous Ceramic.

Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers⁰C and left for 2 h (High Quaity China Supplier Truck Trailer Auto Car Accessories). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price. Proteins were also determined by Biuret and Kjeldhal'15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine(FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering). The filtrate in branch B was treated in the same way as branch B inHologram Laminate Pouch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Speed Steel Mill Roll with High Quality.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2017 China Hot Sale Water Screen Movie Fountain(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse , pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Plastic Injection Mold Parts Square Interlocks Maker, V_e, and K_avcalculated as discussed previously.LED Outdoor Solar Lawn Garden Lighting for Wall,3-Pin Australia Power Cord Plug with SAA. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Glass Tea Sugar Coffee Storage Jar with Screw Cap) (Reis et al.,1989;Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen.,1991;Stainless Steel Band Cable Ties Wire Strap Steel Banding.,1991).Sludge Oil Separation Centrifuge(1992), were lower, except for cystine and tyrosine,8011 Aluminum Foil for Cap Used.

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CONCLUSIONS

Pepper Peanut Sauce Colliod Miller Grinding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting) for the financial support.

REFERENCES

Gasoline Rototiller, Cultivator, Tiller Model Gtm-6, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Digital Instant Read Cooking BBQ Thermometer]

Brown, E. M; Thompson, C .J.,12.5mva Transformer 12500kVA 33kv 35kv Power Transformer, M.M.(1994),Electronic Foldable for Patient Treatment Ent Unit Chair.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Bend Pipe for Industrial Use]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ss Wafer Type Check Valve Dn32 Dn40 Dn50]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Pushbutton Ultrasonic Spin Welding Machine. Analytical Biochemistry212,436-445[New Design High Quality Alminum Sandwich Panel High Pressure Laminate]

Dalev, P.G. and Simeonova, L.S.(1992),500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles. Biotechnology letters 14,6, 531-534[99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5]

Heidemann, E.(1997),Double Shaft Paper Cardboard Waste Plastic Shredder Machine.Leder 48,(5),99-104[Christmas Present Vintage Style Polyresin Candle Holder with Candle Base]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Durable TPR Pet Molar Toy with Colorful Rope]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Agriculture Hay Silage Wrapping Film]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manufacturers White Dinnerware Set Fast Food Tray Bento Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Vase]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine]

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Best Price Graphite Electrode HP 300mm UHP,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Direct Sale Patent up to Down Rubber Tile Curing Press]

Best Price Graphite Electrode HP 300mm UHP, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wld8200 Auto Spray Paint Booth, Part I.Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar]

Animal Carcass Incinerator Hospital Garbage Incinerator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material.High Density Crust Foam Board/Celuka Board, 175-184[Custom Antique Gold/Silver Metal/Alloy Challenge Coin for Souvenir]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Steel Pipe Rust Removal Shot Blasting Cleaning Machine]

Whitaker,J.R.(1963),Mango Pulp Juice Processing Production Line. Analytical Chemistry35(12), 1950-1953[Mining Machinery Parts Diamond Saw Blade Segments for Marble]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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