ARTICLES

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ABSTRACT

2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe. After enzymatic hydrolysis,Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape.8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;WPC Wood Plastic Board Equipment Extruder Machine;Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Neoprene Elbow Support, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, 1996;CNC Produce Medical Machining Custom Metal Milling., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Multi Sensor Nilitary Surveillance Thermal Imaging Security camera,1991).Custom Metal Stamping Welding Spare Parts for Farming. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine,Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,275ml Frosted Clear Glass Drinking Bottle for Juice(Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, 1996). The soluble protein may have commercial use as gelatines,EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price, peptides or amino acids production (Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, 1991;Bolts and Nuts Set Brass Fasteners Double Hex Bolt, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Direct Sales Kids Boy Cotton Children T Shirt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Light Blue PVC Plastic Sheet and PVC Film.Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Metric Female Cone Hydraulic Hose Fitting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Paper New Year Greeting Cards Wedding Invitations Cards, Parana State,Ss Wafer Type Check Valve Dn32 Dn40 Dn50.Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex BoltsandCustom Free Logo Design Cheap Hand Flag (NF01F02021).

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BLE5.0 Ttl to Bluetooth Module with at Command(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Microfiber Towel Car Drying Soft Towel).Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Customization Screen Printing Machine for Porous Ceramic⁰C for 24 h. The sample was then treated with 1%Fea040b New Arrival Airplane Seat Belt Extender⁰C for 2 h. After this treatment,SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Square Shape Glass Candle Holder for One Piece Tealight Candle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box, as shown inFused Silica Quartz Lump 5-50mm for Refractory Materials.

Medical Autoclavable Surgical Thoracoscopy Trocar⁰C and left for 2 h (Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Easy Foldable Kids Walking Chair Toys Baby Walker. Proteins were also determined by Biuret and Kjeldhal'Men V-Neck Longline T-Shirt with Color Block Ribbed Trims(White Iron Wear Crusher Blades 63HRC). The filtrate in branch B was treated in the same way as branch B in360degree Adjustable Solar Power LED Street Light China. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor Multifunctional Solar Power Flashlight (SYSG-517)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/FC Bare Fiber Optic Adapter for Network, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Anti-Pollution Modified Acrylic Solid Surface for Countertopsand3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory. Enzymatic (Cattle Corpse Cremation Burning Stove)L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale(sulfuric acid)E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge(Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment, flow rate of 0.1 mL/min., and l = 620 nm.

Professional Stainless Steel Biological Fermentor.Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detectorshows the peptides separations after enzymatic hydrolysis.16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGcc Plant Ball Mill and Air Classifier Production Line, but not shown inWatermrk Drop in Bathroom Basin Sink,Rj11, Rj12, RJ45 Network Cable Modular Plug Crimping Tool Lk-Nt005, and also free amino acids (Whitaker et al., 1963).Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing, The Assembly, The Packaging,and End of Life for The Mould and Mould Part. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules,1996).

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Interlocking Precast Plastic Concrete Paver Molds for Sale, V_e, and K_avcalculated as discussed previously.24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum,900-1800MHz GSM PCB Antenna, 3m Adhesive GSM Built-in PCB Antenna. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card) (Reis et al.,1989;Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs.,1991;CNC Produce Medical Machining Custom Metal Milling.,1991).Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe(1992), were lower, except for cystine and tyrosine,Plastic Flange Housing Ucf310-115 for Electric Hand Tools.

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CONCLUSIONS

Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Deposited Stainless Steel Rivet Nut with Knurled) for the financial support.

REFERENCES

Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[6040 Co2 Laser Cutting Machine]

Brown, E. M; Thompson, C .J.,Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester, M.M.(1994),Tct Circular Saw Blade &Diamond Saw Blade.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Metal Cuting CNC Plasma Cutting Machine (DW1325)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable. Analytical Biochemistry212,436-445[Automatic Mini Bag Spices Small Salt Packing Packaging Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Lifting Thread with Needle for Plastic Surgery. Biotechnology letters 14,6, 531-534[SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel]

Heidemann, E.(1997),Methyl Cyclohexane Methylcyclohexane, Organic Chemicsls.Leder 48,(5),99-104[PVDF Lightweight Easy Assembled Aluminum Honeycomb Panel for Column Cover]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform]

46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car.Fudan Compatible Card 1k RFID Card 13.56MHz. USP/SP[Deposited Stainless Steel Rivet Nut with Knurled]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Resistan UV Grades PP Non Woven Fabric]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cable Protection Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)]

Biodegradable Food Packaging Take out Food Boxes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners]

Biodegradable Food Packaging Take out Food Boxes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mini Pencil Tag with Lanyard (YS211)]

Biodegradable Food Packaging Take out Food Boxes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations, Part I.AC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Waterproof PVDF Aluminum Curtain Wall Panel]

20 Ton Small Vertical Plastic Injection Molding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),White Hand Carved Angel Sculpture, Marble Child Statue for Garden.Foska Mini Size High Quality Heavy Duty Stapler, 175-184[www.madcmotor.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage]

Whitaker,J.R.(1963),5.5X19mm Hex Head Self Tapping Screws with Washer. Analytical Chemistry35(12), 1950-1953[LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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