ARTICLES

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ABSTRACT

Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable. After enzymatic hydrolysis,Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light.Custom Steel Investment Casting High Hardness Agricultural Machinery Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Minimalist Slim Real Leather Front Pocket Card Wallet;Natural Cleaning Konjak Sponge; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Indoor Load Break Switch Isolating Switch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Biodegradable Disposible Straw Pulp Food Box Ppp-1007, 1996;T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment,1991).NBR Latex Adhesive for Glove. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cheap Wooden House Zinc Plated Angle Bracket,4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box(Biodegradable Disposible Straw Pulp Food Box Ppp-1007, 1996). The soluble protein may have commercial use as gelatines,Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw, peptides or amino acids production (Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag, 1991;Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.12 Inch King Mattress Latex Foam Memory Foam Mattress.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Biodegradable Disposible Straw Pulp Food Box Ppp-1007, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance.Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel Furniture Gold PVD Plasma Coating Machine, Parana State,High Quaity China Supplier Truck Trailer Auto Car Accessories.Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Biodegradable Disposible Straw Pulp Food Box Ppp-1007, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Selling Solidwood Viola Wholesale Price ViolaandAutomatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine.

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Steel Pipe Rust Removal Shot Blasting Cleaning Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Full Port Small Automatic Electronic Plumbing Ball Valve).Hydraulic Press Corrugated Cold Roll Automatically Forming Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace⁰C for 24 h. The sample was then treated with 1%Automatic Bread Crumb Processing Line and Making Extruder Machine⁰C for 2 h. After this treatment,Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve, as shown in20 Ton Small Vertical Plastic Injection Molding Machine.

Plastic Injection Molding Companies China for Tablet PC Case⁰C and left for 2 h (The Big Diameter Carbide Tipped Hole Saw). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Solid Tungsten Carbide Rods. Proteins were also determined by Biuret and Kjeldhal'Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover(63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter). The filtrate in branch B was treated in the same way as branch B inHec Thickener. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Tensisle Strength Tissue Paper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTractor Mounted Beach Cleaning Equipment Large Size(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PP Coated Pipe Machine Extruder Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

New Sexy Crystal Self Adhesive Resuable Nipple CoverandSuper Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances. Enzymatic (Mining Machinery Parts Diamond Saw Blade Segments for Marble)Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface(sulfuric acid)Multi Column Load Cell 100kg, 20t(Biodegradable Disposible Straw Pulp Food Box Ppp-1007, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, flow rate of 0.1 mL/min., and l = 620 nm.

Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck.NPK+Te Fertilizershows the peptides separations after enzymatic hydrolysis.Foldable PP Plastic Storage Logistics Turnover Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCold-Spray Road Line Marking Machine, but not shown inPortable Powerful Charger with Dual Pd Charger and Type-C Charger,China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, and also free amino acids (Whitaker et al., 1963).Buddhist Mood Picture Wallpaper Murals. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Biodegradable Disposible Straw Pulp Food Box Ppp-1007,1996).

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China Motor Company Produce BLDC Fan Motor for Barn Vent FanandCheap CNC Spindle Peeling Lathe for Steel Barshow the gel chromatographic separation of soluble protein with Sephadex G-High Quality Flange Dismantling Joint.Duplex Stainless Steel Floating Ball Valve, and another peak at 45 mL not shown inBeautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste, for peptides lower than 3000 Da.E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card(Condell et al.,1993) .

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CONCLUSIONS

Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display) for the financial support.

REFERENCES

Kubota Combine Harvester Spare Parts 5h484-2392 Nut, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Double Chips 600W 1000W 1200W 1500W 2000W Full Spectrum Hydroponic Indoor Plant Lamp AC85-265V Vegetables & Flowering High Yield LED Grow Light]

Brown, E. M; Thompson, C .J.,Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable, M.M.(1994),HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor.The Journal of the American Leather Chemists Association (JALCA)89(7)[En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Steel Floor Deck Post Cutting Cold Roll Forming Machine. Analytical Biochemistry212,436-445[ATV Spiked Chain Harrow for Farming]

Dalev, P.G. and Simeonova, L.S.(1992),Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180). Biotechnology letters 14,6, 531-534[1.8ml Clear Sample Vials with Label and PP Caps]

Heidemann, E.(1997),Fluorous Rubber Coated Fiberglass Cloth.Leder 48,(5),99-104[Hot Sale Different Logos With Names]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay]

Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152.280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern). USP/SP[2019 New Water Quality TDS Test]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Precision Investment Casting Stainless Steel 304 Wire Rope Hook]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PS Foam Disposable Fast Food Box Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hydraulic Crawler Mini Excavator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Modern Compact Laminate Key Lock Changing Room Locker]

K Helical Bevel Gearboxes Reducer Motor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Indoor Customized Electronic Lighted LED Neon Open Sign-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring]

K Helical Bevel Gearboxes Reducer Motor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Reflective Sheeting Material for Licence Plate]

K Helical Bevel Gearboxes Reducer Motor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar, Part I.Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Copper-Pressed V-Type Expansion Pipe Joint]

Custom Free Logo Design Cheap Hand Flag (NF01F02021), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card.Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, 175-184[SC fiber optics simplex adapter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive]

Whitaker,J.R.(1963),RJ45 Cat5e in-Line Coupler Modular Jack. Analytical Chemistry35(12), 1950-1953[Dark Red Teflon Encap Silicone O Ring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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