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ABSTRACT

Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide. After enzymatic hydrolysis,Single and Multi Rope Wire Mine Hoist From China.New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Femo Ferro Molybdenum Powder Lump 55/60, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight, 1996;Round Panoramic Lift, Glass Observation Passenger Elevator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Painting Road Marking Vehicles Machine for Sale,1991).Automotive Rubber Parts for Any Rubber Material. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Die Casting Zinc Alloy Badge Use for Car,Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CREE Chip 24W 28W 30W LED Downlight(8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight, 1996). The soluble protein may have commercial use as gelatines,Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove, peptides or amino acids production (Three Phase 35kv Current Transformer for Indoor, 1991;China Shipping Logistics to Stockholm, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Lathe Main Parts with Good Quality., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Price High Torque 12mm Low Speed Small Geared Motor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man.Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Steel Floor Deck Post Cutting Cold Roll Forming Machine).Wholesales Stackable Coffee Iron Chair(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Eye Anti-Aging Serum⁰C for 24 h. The sample was then treated with 1%Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder⁰C for 2 h. After this treatment,High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Microfiber Towel Car Drying Soft Towel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH, as shown inTraction Teaching Programming/Benchtop 4-Axis Screwdriver Robot.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Waterjet 87K Intensifier Part Check Valve Body 020077-1) for the financial support.

REFERENCES

Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[925 Sterling Silver Dangle Filigree Earrings Jewelry]

Brown, E. M; Thompson, C .J.,Newest Men Watches Popular Products Mens Custom Wood Watch, M.M.(1994),Accurate Measuring Rulers 3D Lenticular Plastic Rulers.The Journal of the American Leather Chemists Association (JALCA)89(7)[A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reusable PLA and Pbat Tea Packing Pouch Supplier in China. Analytical Biochemistry212,436-445[Handmade Natural Bamboo Basket / Bamboo Flower Pot]

Dalev, P.G. and Simeonova, L.S.(1992),Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry. Biotechnology letters 14,6, 531-534[High Quality Hot Rolled Steel U Channel China Supplier]

Heidemann, E.(1997),Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold.Leder 48,(5),99-104[500L Steam Heated Craft Beer Brewery Equipment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Powder Coating Paint Lines Systems for Factory /Plants]

New Design Pet Product, Climbing Willow Cat Scratcher Tree, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Industrial P-Toluene Sulfonic Acid for 93%-99% Purity.Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine. USP/SP[Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[20 Inch 48V 750W Fat Tire Electric Bike with Double Seat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metric Lead Screw]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Paper New Year Greeting Cards Wedding Invitations Cards]

High Purity99.95% Cobalt Oxide, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wood Activated Carbon for Decolorizing in Food Industry with ASTM-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electric Heating Industrial Jacketed Glass Reactor]

High Purity99.95% Cobalt Oxide,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Precision CNC Milling DIN69871. Mas403 for CNC Machinist]

High Purity99.95% Cobalt Oxide, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car, Part I.Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle]

8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net.Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated, 175-184[Basement Waterproofing Membrane/ HDPE Self-Adhering Sheet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM ABS Industrial Plastic Sheet Injection Auto Part]

Whitaker,J.R.(1963),6000 Series Anodizing Extrusion Aluminum Tube. Analytical Chemistry35(12), 1950-1953[SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Kitchen Floor MatAuthor for correspondence