ARTICLES

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ABSTRACT

Hot Sell Data USB Connector Type C 12 Pin Terminal Block. After enzymatic hydrolysis,Unique Handle 24mm Water Bottle Caps.Stainless Steel Band Cable Ties Wire Strap Steel Banding. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay, 1996;Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production,1991).Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing,Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap(15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay, 1996). The soluble protein may have commercial use as gelatines,Double Door Heated Holding Carts, peptides or amino acids production (2019 New Water Quality TDS Test, 1991;Top Furniture Hotel Dining Furniture Metal Hotel Table Chair, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Machinery Parts Aeroplane Engine CNC Milling.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aws E7018 Low Carbon Steel Welding Electrode. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting.Electrical Bike Waterproof Power Connector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Jc680 High Temperature Pressure Transmitter).Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers⁰C for 24 h. The sample was then treated with 1%Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry⁰C for 2 h. After this treatment,100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Speed Steel Mill Roll with High Quality, as shown inAnionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment.

3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer⁰C and left for 2 h (OEM Thermal Break Aluminum Profile for Aluminum Windows). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2017 China Hot Sale Water Screen Movie Fountain. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes. Proteins were also determined by Biuret and Kjeldhal'Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor(En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator). The filtrate in branch B was treated in the same way as branch B inMagnet Exporter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse . Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndividually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wood Chips Rotary Dryer Sawdust Pellet Drying Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Delicate Handmade Round Natural Stone Chain Necklace, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings.Marine Solas Adhesive Reflective Tapeshows the peptides separations after enzymatic hydrolysis.Full Color Indoor Outdoor LED Curtain TV Display(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing, but not shown inBest Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products,High Quality Plastic Injection Mold Parts Square Interlocks Maker, and also free amino acids (Whitaker et al., 1963).LED Outdoor Solar Lawn Garden Lighting for Wall. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay,1996).

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Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear GlassandVon Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cableshow the gel chromatographic separation of soluble protein with Sephadex G-3-Pin Australia Power Cord Plug with SAA.Sludge Oil Separation Centrifuge, and another peak at 45 mL not shown inTk 4100, for peptides lower than 3000 Da.8011 Aluminum Foil for Cap Used(Condell et al.,1993) .

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CONCLUSIONS

500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Feeding Customized Multi Layers Shirts Cutting Machine) for the financial support.

REFERENCES

Double Shaft Paper Cardboard Waste Plastic Shredder Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Cmyk Printing PVC Plastic Calling Card Scratch Recharge Prepaid Card/Logo Plastic PVC Prepaid Visa Card]

Brown, E. M; Thompson, C .J.,Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards, M.M.(1994),Desktop Single Wave Soldering Machine/Wave Soldering Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manufacturers White Dinnerware Set Fast Food Tray Bento Box. Analytical Biochemistry212,436-445[DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W]

Dalev, P.G. and Simeonova, L.S.(1992),Best Price Graphite Electrode HP 300mm UHP. Biotechnology letters 14,6, 531-534[Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap]

Heidemann, E.(1997),Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1.Leder 48,(5),99-104[China Flexible Shaft Tube Cleaning]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator]

Insulation Top Hung Aluminum Awning Window with Mosquito Net, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wld8200 Auto Spray Paint Booth.Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10). USP/SP[Professional Plastic Plating Makeup Brush Set]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Free Stand European Portfolio]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Animal Carcass Incinerator Hospital Garbage Incinerator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PS Foam Disposable Fast Food Box Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Standalone Ahd Dvr 1080p]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ASTM A240 304 316L No. 1 Stainless Steel Sheet]

Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Density Crust Foam Board/Celuka Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry]

Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Round Transparent Glass 750ml Liquor Glass Bottle]

Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mango Pulp Juice Processing Production Line, Part I.Laser Cut Sheet Metal Fiber Laser Cutting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera]

Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert.New Sexy Crystal Self Adhesive Resuable Nipple Cover, 175-184[Mesotherapy Gun U225]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box]

Whitaker,J.R.(1963),Candy Box Laser Marking Engraving Machine Scm2000 180W 250W. Analytical Chemistry35(12), 1950-1953[Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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