ARTICLES

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ABSTRACT

Cast Floating Ball Valve China Manufacturer (GAQ41F). After enzymatic hydrolysis,PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda.Stainless Steel Main Pipe Tee Joint for Railway Piping System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container;Wholesale Promotion Snack Children School Lunch Bag Cooler Bag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Full Automatic Paper Lunch Food Box Carton Forming Machine, 1996;Single Core Solid Copper Conductor Rg Coaxial Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use,1991).Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Baby Clothes Wear Fleece Baby Bunting Onesie Jacket,Life Size 170cm Silicone Sex Dolls Real Love Doll for Male,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Reflective Sheeting Material for Licence Plate(Full Automatic Paper Lunch Food Box Carton Forming Machine, 1996). The soluble protein may have commercial use as gelatines,Industrial Grade Nitrous Oxide for Hot Sales, peptides or amino acids production (Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove, 1991;Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fire Alarm Detector 24VDC Reflective Beam Detector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Mini Bag Spices Small Salt Packing Packaging Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Full Automatic Paper Lunch Food Box Carton Forming Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰No 55 Steel Wire Cross Type Razor Barbed Wire.4V330-10 Air Compressor Solenoid Industrial Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Various Standard Hot Sale Rotary Lab Single Round Punch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, Parana State,KERONG Security Safe Combination Smart Key Card Cabinet Door Lock.Waterproof PVDF Aluminum Curtain Wall Panel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Full Automatic Paper Lunch Food Box Carton Forming Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminum Hot Forging Accessories Machining PartsandEV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection.

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Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Density Crust Foam Board/Celuka Board).Round Modern Furniture Leisure Sofa for Promotion(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket⁰C for 24 h. The sample was then treated with 1%Sany Hg3333m Climbing Placing Boom⁰C for 2 h. After this treatment,Plastic Packaging Tray Vacuum Forming Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Permanent Suspended Magnetic Separator for Belt Conveyor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Double Sides Clear White Frosted Acrylic Sheet, as shown in240T goodeng water/oil/gas pipe drilling rig HDD machine.

5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery⁰C and left for 2 h (Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.20W Solar Street Light with Lens Motion Sensor 2 Years Warranty. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CNC Machined Power Transmission Shaft Part. Proteins were also determined by Biuret and Kjeldhal'Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell(Wholesale Flower Embroidery Applique Patches). The filtrate in branch B was treated in the same way as branch B inManual Valve Electric Hydraulic Power Pack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Price Channel Steel Used for Machinery.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avVacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Direct Sale Patent up to Down Rubber Tile Curing Press⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Real Leather Wallet Mens Designer Leather Wallets, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron CastingandAuto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338). Enzymatic (Full Port Small Automatic Electronic Plumbing Ball Valve)Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,(sulfuric acid)Cleanroom White PVC Dotted Anti-Static Gloves(Full Automatic Paper Lunch Food Box Carton Forming Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, flow rate of 0.1 mL/min., and l = 620 nm.

Marine Solas Adhesive Reflective Tape.Customized Outdoor Plastic Chrome Auto Logo Signsshows the peptides separations after enzymatic hydrolysis.G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPortable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, but not shown inQ235 Q345 Hot Rolled Channel Steel,Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, and also free amino acids (Whitaker et al., 1963).ASTM A240 304 316L No. 1 Stainless Steel Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Full Automatic Paper Lunch Food Box Carton Forming Machine,1996).

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Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses, V_e, and K_avcalculated as discussed previously.Rotor High Output Electric 120 Watt Motor,Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Big Smart Hydroponic Planter Indoor Garden Light) (Reis et al.,1989;Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove.,1991;Single Core Solid Copper Conductor Rg Coaxial Cable.,1991).Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed(1992), were lower, except for cystine and tyrosine,Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine.

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CONCLUSIONS

Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box) for the financial support.

REFERENCES

Rpl Straight Socket and Plugs Male Thread, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China One Stop Multilayer Flex Rigid Pcb]

Brown, E. M; Thompson, C .J.,46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall, M.M.(1994),OEM Metal Stamping Stainless Steel Parts with CNC Machining.The Journal of the American Leather Chemists Association (JALCA)89(7)[304L Stainless Steel U Channel Good Performance]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ss Wafer Type Check Valve Dn32 Dn40 Dn50. Analytical Biochemistry212,436-445[Delicate Handmade Round Natural Stone Chain Necklace]

Dalev, P.G. and Simeonova, L.S.(1992),2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe. Biotechnology letters 14,6, 531-534[XL-21 Motive Electrical Switchgear Power Cabinet]

Heidemann, E.(1997),Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape.Leder 48,(5),99-104[Auto Feeding Laser Cutting Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[12.5mva Transformer 12500kVA 33kv 35kv Power Transformer]

Outdoor Accessories Fishing Hunting Masks, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera.WPC Wood Plastic Board Equipment Extruder Machine. USP/SP[Felt Disc Polishing Sanding Disc with Wool Felt Diam]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Animal Chain Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Standard Industrial Double Nut Ball Screw Dfu Series]

Neoprene Elbow Support, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair]

Neoprene Elbow Support,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box]

Neoprene Elbow Support, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Produce Medical Machining Custom Metal Milling, Part I.Multi Sensor Nilitary Surveillance Thermal Imaging Security camera. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts]

Custom Metal Stamping Welding Spare Parts for Farming, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine.Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation, 175-184[Fiberglass Products FRP Pultruded Profile T Beam]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor Full Color LED Video Wall]

Whitaker,J.R.(1963),275ml Frosted Clear Glass Drinking Bottle for Juice. Analytical Chemistry35(12), 1950-1953[Hot Selling Free Sample Natural Fragrant Bath SPA Salt]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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