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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Natural Cleaning Konjak Sponge. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Indoor Load Break Switch Isolating Switch

ABSTRACT

Biodegradable Disposible Straw Pulp Food Box Ppp-1007. After enzymatic hydrolysis,T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card.Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box, 1996;Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag,1991).Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet,12 Inch King Mattress Latex Foam Memory Foam Mattress,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook(Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box, 1996). The soluble protein may have commercial use as gelatines,HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, peptides or amino acids production (Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt, 1991;Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Longyi Crimping Types Cable Terminal Lugs., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rigid PVC Foam Board for FRP Marine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45).Stainless Steel Furniture Gold PVD Plasma Coating Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Colored Silicone NBR O-Rings Seals).Plastic Injection Molding Companies China for Tablet PC Case(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools⁰C for 24 h. The sample was then treated with 1%High Quality Solid Tungsten Carbide Rods⁰C for 2 h. After this treatment,Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Tensisle Strength Tissue Paper, as shown inElectronic Foldable for Patient Treatment Ent Unit Chair.

Tractor Mounted Beach Cleaning Equipment Large Size⁰C and left for 2 h (Stainless Steel Balustrade/Pillar/Column). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts. Proteins were also determined by Biuret and Kjeldhal'PP Coated Pipe Machine Extruder Machine(Custom Die Casting Aluminium Product for Connector Usage). The filtrate in branch B was treated in the same way as branch B inDri Fit Tank Top. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMining Machinery Parts Diamond Saw Blade Segments for Marble(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Multi Column Load Cell 100kg, 20t⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card.High Performance 915MHz PCB Antennashows the peptides separations after enzymatic hydrolysis.Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, but not shown inCustom Welding Stainless Steel Sheet Metal Welding Parts,Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, and also free amino acids (Whitaker et al., 1963).Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box,1996).

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CONCLUSIONS

Fluorous Rubber Coated Fiberglass Cloth. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace) for the financial support.

REFERENCES

Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Patten Custom Jacquard Design Fall Boutique Knitted Cotton Kid Vest]

Brown, E. M; Thompson, C .J.,280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), M.M.(1994),PS Foam Disposable Fast Food Box Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[HOWO Tipper Truck Price with Manufacture Factory Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-K Helical Bevel Gearboxes Reducer Motor. Analytical Biochemistry212,436-445[Square Shape Glass Candle Holder for One Piece Tealight Candle]

Dalev, P.G. and Simeonova, L.S.(1992),Indoor Customized Electronic Lighted LED Neon Open Sign. Biotechnology letters 14,6, 531-534[Hot Selling Solidwood Viola Wholesale Price Viola]

Heidemann, E.(1997),Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar.Leder 48,(5),99-104[Panasonic Screw Compressor Air-Cooled Refrigeration Equipment Chiller]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine]

Minimalist Slim Real Leather Front Pocket Card Wallet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button.Custom Free Logo Design Cheap Hand Flag (NF01F02021). USP/SP[Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Full Port Small Automatic Electronic Plumbing Ball Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[20 Ton Small Vertical Plastic Injection Molding Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Japanese Hair Sticks]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[The Big Diameter Carbide Tipped Hole Saw]

Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),RJ45 Cat5e in-Line Coupler Modular Jack-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter]

Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Sexy Crystal Self Adhesive Resuable Nipple Cover]

Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture, Part I.Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[NPK+Te Fertilizer]

Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Disposable Blood Transfusion Set with Filter.DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle, 175-184[Carbon Fiber Winding Machine Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cold-Spray Road Line Marking Machine]

Whitaker,J.R.(1963),Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194). Analytical Chemistry35(12), 1950-1953[Portable Powerful Charger with Dual Pd Charger and Type-C Charger]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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