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ABSTRACT

EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012). After enzymatic hydrolysis,60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light.Universal Mirco Forklift Car Bus Truck Seats Switch Sensors. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder;Mexico Single Expansion Anchor with YZP; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet, 1996;Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Whole Absorbtion Flexo Printing Soltting Die Cutting Machine,1991).Factory Price 4X6 Acrylic Magnetic Photo Frame. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,200m Diesel Oil Hydraulic Water Well Drilling Machine,Custom Cmyk Printing Cake Gift Packing Box with Lid,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Air Purification Activated Alumina(SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet, 1996). The soluble protein may have commercial use as gelatines,Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6), peptides or amino acids production (Resistan UV Grades PP Non Woven Fabric, 1991;New Scba Breathing Apparatus Equipment with Ce Certification, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;FRP Pultruded Pipe or GRP Profile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.6090 CO2 Sealed Glass Cooling Laser Cutting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper.Outdoor Fitness Equipment Swing (JMH-03)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sheet Metal Distribution Cabinet with High Quality(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant, Parana State,Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover.Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom Logo Stainless Steel Quality Wrist Quartz Watch for MenandIndoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale.

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High Output Plastic Rubber Filing Masterbatch Extruder Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing).High Precision Mold Manufacturer Plastic Injection Mould(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Wholesale Oxide Aluminium Black Sand Blast Media⁰C for 24 h. The sample was then treated with 1%Welded Stud Anchor Chain for Ship⁰C for 2 h. After this treatment,SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Line Scan Uvss System Portable Under Vehicle Surveillance's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Folding Steel Manual Wheelchair with Chrome Frame, as shown inAMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle.

Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel⁰C and left for 2 h (Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fashion Style Lady Alloy Quartz Watch with Simple Dial. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Rooftop HVAC Packaged Units (RTU) Amrta Manufacture. Proteins were also determined by Biuret and Kjeldhal'Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box(Food Grade Customized Metal Candy Can Square Mint Tin Box). The filtrate in branch B was treated in the same way as branch B inEx Officio Bug Repellent Clothing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Building Decoration Material Acrylic Dispersant for Coating.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDiamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wrinkle Effect Crease UV Drying Machine UV Curing System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Savingand20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory. Enzymatic (Tefude Feeding and Packaging Line for Food with Tray)Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts(sulfuric acid)Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine(SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CMP No Nc Metal Switch Black 25mm Illuminated Push Button, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CMP No Nc Metal Switch Black 25mm Illuminated Push Button, flow rate of 0.1 mL/min., and l = 620 nm.

Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks.Hot Sale 840 Type PPGI Color Steel Roofing Sheetsshows the peptides separations after enzymatic hydrolysis.Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYbz-E16f20 Vane Steering Hydraulic Pumps, but not shown inColored Silicone NBR O-Rings Seals,Felt Disc Polishing Sanding Disc with Wool Felt Diam, and also free amino acids (Whitaker et al., 1963).13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet,1996).

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Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, V_e, and K_avcalculated as discussed previously.Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength,Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail) (Reis et al.,1989;Resistan UV Grades PP Non Woven Fabric.,1991;Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope.,1991).Colorful Braided Fabric Cable Round(1992), were lower, except for cystine and tyrosine,Universal Wholesale Hot Sale Custom Blank Keys.

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CONCLUSIONS

Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting) for the financial support.

REFERENCES

Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hose Barb Connections]

Brown, E. M; Thompson, C .J.,Waw 8 Ton Logistics Light Truck for Transportation, M.M.(1994),Hot Forging Lathe Spindle Forged Spindle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Comfortable Men′s Winter Leather Snow Boots]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Laser ID Card Rigid PET Printing Sheet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale Chic Straw Braid Floppy Hat with Print Tape. Analytical Biochemistry212,436-445[ Hamburgers]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply. Biotechnology letters 14,6, 531-534[Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated]

Heidemann, E.(1997),Lamella Clarifier in Sewage Tretament Plant for Water Purification.Leder 48,(5),99-104[www.kundetools.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte]

3D Creative Design Metal Medal Promotion Gift of Sport, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),BMC (DMC) / SMC Compression Moulding.Ring Caps &Plastic Tube Pop Novelties. USP/SP[High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[HOWO Tipper Truck Price with Manufacture Factory Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Running Mobile Arm Pack for Outdoor Sports]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell]

Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dual Plastic 9 Volt Battery Clips Connector with Wire-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Square Shape Glass Candle Holder for One Piece Tealight Candle]

Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Selling Solidwood Viola Wholesale Price Viola]

Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Precision Ball Screw with Two Nut, Part I.EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine]

Low Density Cleaner in Paper Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada.Customized Stainless Steel Laser Cutting, 175-184[18 Diamond Cutting Disc Saw Blade for Pakistan Marble]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System]

Whitaker,J.R.(1963),Price of a Retail Supermarket Push Cart for Sale. Analytical Chemistry35(12), 1950-1953[Full Port Small Automatic Electronic Plumbing Ball Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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