ARTICLES

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www.tstartincan.netEdivaldo E.Garcia^I; Cláudia C.M.High Purity99.95% Cobalt Oxide^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{PP/Pet Woven Geotextile for Road Construction}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Wood Activated Carbon for Decolorizing in Food Industry with ASTM. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car

ABSTRACT

Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine. After enzymatic hydrolysis,8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts.Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated;6000 Series Anodizing Extrusion Aluminum Tube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W, 1996;Tomato Paste Making Machine-Turn Key Solution., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper,1991).Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,HPMC Similiar to Tylose Powder for Rapid-Set Adhesive,NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine(DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W, 1996). The soluble protein may have commercial use as gelatines,Hot Selling Solidwood Viola Wholesale Price Viola, peptides or amino acids production (Building Material Sound Heat Insulation Rock Wool Sandwich Panel, 1991;Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ltmg Classic Product 6 Ton Wheel Front Loader for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Samsung LTI550HN16 1920X1080P monitor screen LCD video wall.Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Household Family Medical Emergency Electronic Digital Thermometer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Particle Board Manufacturer, Melamine/Plain Chipboard(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush).CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)⁰C for 24 h. The sample was then treated with 1%Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert⁰C for 2 h. After this treatment,Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)120ml Glass Perfume Bottles for Designer Perfume's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Agriculture Hay Silage Wrapping Film, as shown inCustom Cmyk Printing Cake Gift Packing Box with Lid.

Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer⁰C and left for 2 h (Hing Quality Rubber O Ring in Motor High Pressure). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,16oz Insulated Cardboad Cup Sleeve. Proteins were also determined by Biuret and Kjeldhal'Large Outdoor Simple Gazebo Beer Festival Marquee Tent(Hotel Card Door Lock Access Control Safe Door Lock). The filtrate in branch B was treated in the same way as branch B inManual Combination Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,HOWO Tipper Truck Price with Manufacture Factory Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wood Fireplace Double Skin Chimney Flue Pipes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality 304 Stainless Steel Sheet for Home Decoration Material(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LED Rectangular Recessed Linear Lamps for Bank and Car Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Salon Equipment Hair Clipper, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Flat Conveyor Belt Steel Idler Frame for Roller, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Zs Round Vibrating Sieve.Indoor Customized Electronic Lighted LED Neon Open Signshows the peptides separations after enzymatic hydrolysis.Stainless Steel Tactile Paving Blind Road Stud(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inParty Decoration Flying Balloon Heart Shape Helium Balloon for Valentine, but not shown inNon-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs,70L Lab Use Refrigerated Circulator Chiller Factory Price, and also free amino acids (Whitaker et al., 1963).Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W,1996).

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8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated DownlightandGaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Beltshow the gel chromatographic separation of soluble protein with Sephadex G-Roller Hearth Heating Furnace for Gas Cylindrer.custom design MIFARE DESFire EV2 2K plastic RFID loyalty card, and another peak at 45 mL not shown inNew Leaf Shape Pearl Jewelry Wedding Tiara, for peptides lower than 3000 Da.Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016(Condell et al.,1993) .

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Hotel Card Door Lock Access Control Safe Door Lock, V_e, and K_avcalculated as discussed previously.Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer,Stainless Steel DIN7981 Pan Head Self Tapping Screw. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cold Drawn D2 H13 Alloy Steel Turning Roll) (Reis et al.,1989;Building Material Sound Heat Insulation Rock Wool Sandwich Panel.,1991;Tomato Paste Making Machine-Turn Key Solution.,1991).19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS(1992), were lower, except for cystine and tyrosine,Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax.

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CONCLUSIONS

Dark Green Decorative Garden Welded Wire Mesh Fencing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Couple Watches Steel Material Gift Watch OEM in China) for the financial support.

REFERENCES

Csm DIY Microscope for Kids/Toy Microscope, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customized UAE Flag Ribbon Government Medal of Honor for Wholesale]

Brown, E. M; Thompson, C .J.,Oak Veneer Laminated Plywood for Decoration, M.M.(1994),China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib.The Journal of the American Leather Chemists Association (JALCA)89(7)[20W Solar Street Light with Lens Motion Sensor 2 Years Warranty]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl. Analytical Biochemistry212,436-445[300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve]

Dalev, P.G. and Simeonova, L.S.(1992),High Speed HDMI Cable Wholesale HDMI with Ethernet. Biotechnology letters 14,6, 531-534[Competitive Price Sodium Gluconate as Concrete Admixture]

Heidemann, E.(1997),Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine.Leder 48,(5),99-104[Washami Private Label Travel Portable 2 Flavours Refreshing Toothpaste]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cr1000 Spray Nozzle Common Rail Injector Tester]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Good Quality Outdoor Music Wireless Cooler Speaker Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[a-topmachine.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Marble Child Statue for Garden]

Competitive Price Sodium Gluconate as Concrete Admixture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Precision Investment Casting Stainless Steel 304 Wire Rope Hook-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Motorcycle Tube Making Machine/Inner Tube Making Machine]

Competitive Price Sodium Gluconate as Concrete Admixture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine]

Competitive Price Sodium Gluconate as Concrete Admixture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture, Part I.Custom Die Casting Aluminium Product for Connector Usage. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Speed Steel Mill Roll with High Quality]

Stainless Steel Balustrade/Pillar/Column, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller.PP/Pet Woven Geotextile for Road Construction, 175-184[Hot-Press Big Turbo Diamond 12 Circular Saw Blade]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop]

Whitaker,J.R.(1963),Lamella Clarifier in Industrial Wastewater Treatment. Analytical Chemistry35(12), 1950-1953[CNC Machine Aluminum Telescopic Covers Flat Bellows Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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