ARTICLES

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ABSTRACT

600X600 mm Ugr 19 LED Panel Lights. After enzymatic hydrolysis,Good Quality Hot Tube8 Japanese Stainless Steel Tube 666.Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, 1996;New Design High Quality Alminum Sandwich Panel High Pressure Laminate., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Steel Setractable Rail Protection Cover,1991).Natural Birch F&B Veneer Furniture Plywood. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator,High Quality Tire Repair Tool Kit, Tire Repair Strip,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Performance 915MHz PCB Antenna(10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, 1996). The soluble protein may have commercial use as gelatines,GRP/FRP Profile Structures/Fiberglass Pultrusion Product, peptides or amino acids production (Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, 1991;High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Metal Material Products for Punching Stamping Part.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller.Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Spark Resistant Construction Axial Fan with Fixed Pitch Blade(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CMP No Nc Metal Switch Black 25mm Illuminated Push Button).Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home⁰C for 24 h. The sample was then treated with 1%25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)⁰C for 2 h. After this treatment,Precision CNC Milling DIN69871. Mas403 for CNC Machinist. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Building Material of Granite Ceramic Wall Tile for Wall Decoration, as shown inHigh Pulling Force Rotatable Rare Earth Crane Magnet Hook.

3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine⁰C and left for 2 h (New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop. Proteins were also determined by Biuret and Kjeldhal'Precision Customized CNC Machined Mild Steel Flange for Automobile(Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car). The filtrate in branch B was treated in the same way as branch B inDough Sheeter Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Metal Container Packaging for Edible Oil Tin Cans Storage Boxes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMelee Custom Plastic Mould New Design(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Copper-Pressed V-Type Expansion Pipe Joint⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber, pH 3.25., mobile phase B: buffer solution of 0.01 mol/White Desiccant Bag Silica Gel with Indicated Orange Bead, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Nbm High Quality Auto Part Factory Price 6pk2310. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light) for the financial support.

REFERENCES

Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tufted Carpet Tiles Nylon Face PVC Backing 50X50cm Carpet Factory in China Wall Carpet Padding Alaska]

Brown, E. M; Thompson, C .J.,High Quality Mini LED Flood Light SMD Flood Light 50W, M.M.(1994),Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead.The Journal of the American Leather Chemists Association (JALCA)89(7)[24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass. Analytical Biochemistry212,436-445[2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush]

Dalev, P.G. and Simeonova, L.S.(1992),Professional Manufacture Metal Medal Promotional Gift. Biotechnology letters 14,6, 531-534[Good Quality Hot Tube8 Japanese Stainless Steel Tube 666]

Heidemann, E.(1997),Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving.Leder 48,(5),99-104[Seamless Steel Rectangular Tube of 350X250mm Thickness 19.0mm]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine]

High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Singlemode Su Upc Fiber Optic Fast Connector.Roller Skating Sneakers Shoes for Children (8083). USP/SP[Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Sale Various Customized Square 6063 Extruded Aluminum Profile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[304L Stainless Steel U Channel Good Performance]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom]

Electric Heating Industrial Jacketed Glass Reactor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),40X-1600X Biological Microscope Xsz-107V LED Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[LED Rectangular Recessed Linear Lamps for Bank and Car Sale]

Electric Heating Industrial Jacketed Glass Reactor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine]

Electric Heating Industrial Jacketed Glass Reactor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:6063 Aluminum Coil for Commercial Vehicle Floor, Part I.Silicon Carbide Foundry Porous Ceramic Foam Filter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs]

1200X1000X810 Strong Collapsible Plastic Containers for Storage, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Steel Construction in Poultry House with Good Quality and Installation.Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White, 175-184[Garden Air Hose PVC Covered Colorful Rubber Hose]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools]

Whitaker,J.R.(1963),10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle. Analytical Chemistry35(12), 1950-1953[Steel Floor Deck Post Cutting Cold Roll Forming Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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