ARTICLES

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ABSTRACT

Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas. After enzymatic hydrolysis,Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength.Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Colorful Braided Fabric Cable Round;Universal Wholesale Hot Sale Custom Blank Keys; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, 1996;Waw 8 Ton Logistics Light Truck for Transportation., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Forging Lathe Spindle Forged Spindle,1991).Hot Sale Chic Straw Braid Floppy Hat with Print Tape. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply,Lamella Clarifier in Sewage Tretament Plant for Water Purification,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,BMC (DMC) / SMC Compression Moulding(Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, 1996). The soluble protein may have commercial use as gelatines,Ring Caps &Plastic Tube Pop Novelties, peptides or amino acids production (Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet, 1991;Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dual Plastic 9 Volt Battery Clips Connector with Wire., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Precision Ball Screw with Two Nut.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Low Density Cleaner in Paper Making Machine.En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Stainless Steel Laser Cutting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Price of a Retail Supermarket Push Cart for SaleAlcalase2.5 L DX,Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Handmade Natural Bamboo Basket / Bamboo Flower Pot(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Stainless Steel Handrail Bar Holder for 16mm Pipe, Parana State,240T goodeng water/oil/gas pipe drilling rig HDD machine.Shac Flange Linear Bearing, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)andChina Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal.

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China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Dental Material Dental PMMA Block From China).Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CNC Router for Engraving Cutting Carving Drilling Milling⁰C for 24 h. The sample was then treated with 1%Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine⁰C for 2 h. After this treatment,Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Hot Rolled Steel U Channel China Supplier, as shown inPrecision Customized CNC Machined Mild Steel Flange for Automobile.

M Type Metallic Yarn Lurex Yarn Embroidery Yarn⁰C and left for 2 h (Calcined Petroleum Coke as Best Recarburizer in Iron Industry). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck. Proteins were also determined by Biuret and Kjeldhal'500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment(Plastic Pushbutton Ultrasonic Spin Welding Machine). The filtrate in branch B was treated in the same way as branch B inFiber Laser Tube Cutter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Anti-Pollution Modified Acrylic Solid Surface for Countertops. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDouble Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.800lb 1/2 A105 Thread Forged Steel Globe Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption ElementsandCNC Machining Metal Mechanical Engineering Parts. Enzymatic (Low Sulphur Calcined Carbon Raiser)Hot Selling Free Sample Natural Fragrant Bath SPA Salt(sulfuric acid)Custom FSTN Va COB Cog Segment LCD Display Module(Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Jc680 High Temperature Pressure Transmitter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Jc680 High Temperature Pressure Transmitter, flow rate of 0.1 mL/min., and l = 620 nm.

Deep Hole Cylinder Boring Machine for Sale.Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150Lshows the peptides separations after enzymatic hydrolysis.Hot Selling 202 Stainless Steel Coil(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in6063 Aluminum Coil for Commercial Vehicle Floor, but not shown inHeated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People,NPK 16X Hammer Spare Parts Racing Forged Piston, and also free amino acids (Whitaker et al., 1963).2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30,1996).

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4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair MachineandThree Phase 35kv Current Transformer for Indoorshow the gel chromatographic separation of soluble protein with Sephadex G-Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector.Mobile Hydraulic Concrete Placing Boom, and another peak at 45 mL not shown inStainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, for peptides lower than 3000 Da.Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700(Condell et al.,1993) .

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CONCLUSIONS

Kt Board Acrylic CCD Camera CNC Knife Cutting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate) for the financial support.

REFERENCES

High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale 5g Smartphone for Huawei Mate 30 PRO Cell Phone Mate 20 PRO Smart Phone Unlocked The Original Dual SIM Mobile Phone]

Brown, E. M; Thompson, C .J.,Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, M.M.(1994),Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks.The Journal of the American Leather Chemists Association (JALCA)89(7)[Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3. Analytical Biochemistry212,436-445[Industrial Cast Steel Flanged One Way Swing Check Valve Nrv]

Dalev, P.G. and Simeonova, L.S.(1992),China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part. Biotechnology letters 14,6, 531-534[Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price]

Heidemann, E.(1997),Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box.Leder 48,(5),99-104[Wireless WiFi Smart Socket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter]

Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz.Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator. USP/SP[High Speed HDMI Cable Wholesale HDMI with Ethernet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ SLA Shoes Prototyping for Auto Accessory]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Carbon Steel Stainless Steel Countersunk Head Hollow Bolt: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pet Automatic Water Dispenser Feeder Cat Dog Water Dispenser Dog Bowl Pet Food Slow Food Bowl]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sludge Oil Separation Centrifuge]

Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post]

Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Real Leather Wallet Mens Designer Leather Wallets]

Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable, Part I.5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk]

Clear PVC Roof Frame Tent in South Africa, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511).Stainless Steel Bend Pipe for Industrial Use, 175-184[Eye Bolts M30 DIN580 Anchor Lifting Rigging]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ 60 Hz]

Whitaker,J.R.(1963),Ybz-E16f20 Vane Steering Hydraulic Pumps. Analytical Chemistry35(12), 1950-1953[Stone Cracking Rock Cutting Cement]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush SpongeAuthor for correspondence