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ABSTRACT

Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine. After enzymatic hydrolysis,16A/24A High Speed Wire Cable Braiding Machine.Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050);20 Inch 48V 750W Fat Tire Electric Bike with Double Seat; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Agriculture Apple Orchard Reflective Pet Film, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04), 1996;Small Boat Deck Knuckle Boom Ship Pedestal Crane., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (115mm Segmented Saw Blade for Concrete Cutting,1991).Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Round Transparent Glass 750ml Liquor Glass Bottle,3D Creative Design Metal Medal Promotion Gift of Sport,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ad-L13 Black Perfume Plastics Vial Bottle 10ml(High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04), 1996). The soluble protein may have commercial use as gelatines,Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, peptides or amino acids production (EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012), 1991;60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Universal Mirco Forklift Car Bus Truck Seats Switch Sensors., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mexico Single Expansion Anchor with YZP. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe.SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Air Purification Activated Alumina, Parana State,1.0g Water and Air Ozone Generator with Silicone Tube.Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStone PVC Luxury Vinyl Plank Spc Flooring with V GrooveandHigh Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511).

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Resistan UV Grades PP Non Woven Fabric(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fully Automatic Water Bottle Lids Labeling Machine).New Scba Breathing Apparatus Equipment with Ce Certification(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%FRP Pultruded Pipe or GRP Profile⁰C for 24 h. The sample was then treated with 1%6090 CO2 Sealed Glass Cooling Laser Cutting Machine⁰C for 2 h. After this treatment,Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor Fitness Equipment Swing (JMH-03), as shown inCustom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody.

Sheet Metal Distribution Cabinet with High Quality⁰C and left for 2 h (Screw/Countersunk Head Screw 6 Ribs with Wing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice. Proteins were also determined by Biuret and Kjeldhal'Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting(HPMC Hydroxy Propyl Methyl Cellulose Additives). The filtrate in branch B was treated in the same way as branch B inOriginal Cummins 6CT Piston Spare Parts (3917707). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Metal Animal Longhorn Skull Pendant Longhorn Skull Charm.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Precision Mold Manufacturer Plastic Injection Mould⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Wholesale Oxide Aluminium Black Sand Blast Media, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Welded Stud Anchor Chain for Ship, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency TechnologyandSGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry. Enzymatic (Line Scan Uvss System Portable Under Vehicle Surveillance)Folding Steel Manual Wheelchair with Chrome Frame(sulfuric acid)Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel(High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fashion Style Lady Alloy Quartz Watch with Simple Dial, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fashion Style Lady Alloy Quartz Watch with Simple Dial, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (200m Diesel Oil Hydraulic Water Well Drilling Machine) for the financial support.

REFERENCES

CMP No Nc Metal Switch Black 25mm Illuminated Push Button, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Women Dresses Thick Slimming Witer Warm Tights Sexy Shiny Seamless Tights Leggings]

Brown, E. M; Thompson, C .J.,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, M.M.(1994),Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dual Plastic 9 Volt Battery Clips Connector with Wire]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Felt Disc Polishing Sanding Disc with Wool Felt Diam. Analytical Biochemistry212,436-445[Fashion Women Hair Accessories Hair Bow Headband for Girls]

Dalev, P.G. and Simeonova, L.S.(1992),13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System. Biotechnology letters 14,6, 531-534[E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card]

Heidemann, E.(1997),Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable.Leder 48,(5),99-104[Forging Press Made Forging Parts for Metal Forging Machinery]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine]

Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth.240T goodeng water/oil/gas pipe drilling rig HDD machine. USP/SP[ Cnice Brand Toothpaste with Oral Refreshing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Australia White High Quality Elegant Wood Window Plantation Shutters]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Shipping Logistics to Stockholm]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[excaliburgenerator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cleanroom White PVC Dotted Anti-Static Gloves]

Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface]

Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Metal Material Products for Punching Stamping Part]

Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Colorful Braided Fabric Cable Round, Part I.Universal Wholesale Hot Sale Custom Blank Keys. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fashion High Quality Head Layer Genuine Leather Men′s Wallet]

Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30.Waw 8 Ton Logistics Light Truck for Transportation, 175-184[Top Quality Good Price Food Grade Oil Drilling Xanthan Gum]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price]

Whitaker,J.R.(1963),Hot Forging Lathe Spindle Forged Spindle. Analytical Chemistry35(12), 1950-1953[Universal Mirco Forklift Car Bus Truck Seats Switch Sensors]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure SpringAuthor for correspondence