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ABSTRACT

Custom Good Quality Outdoor Music Wireless Cooler Speaker Box. After enzymatic hydrolysis,OEM Viton Mechanical Rubber Oil Seal.Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hsac Shac Linear Guideway, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses, 1996;Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pure Water Cutting Head Assy for Waterjet Cutting Machine,1991).Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Latest Lace Fabric Design Logo Flag Embroidery Patch,China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic(Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses, 1996). The soluble protein may have commercial use as gelatines,Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05, peptides or amino acids production (New H Type Battery Cage System for Layer Chicken Farm, 1991;Calcined Petroleum Coke as Best Recarburizer in Iron Industry, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;X15 Power Monitor Two Way Live Stage Monitor Speaker System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Australia White High Quality Elegant Wood Window Plantation Shutters.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Big Smart Hydroponic Planter Indoor Garden Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion.Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Selling Custom Sheet Metal Fabrication(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

100% Latex Free Endotracheal Tube with PVC Material, Parana State,Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry.Neodymium Magnet Pot Assembly with Strong Weight, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Precision Mold Manufacturer Plastic Injection MouldandWPC Wood Plastic Board Equipment Extruder Machine.

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Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS).China Manufacturer of Venlo PC or Venlo Glass Greenhouse(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Artnet Node Artnet to DMX Controller⁰C for 24 h. The sample was then treated with 1%6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes⁰C for 2 h. After this treatment,Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CH Series Pid Intelligent Temperature Controller CH102's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Householding High Precision Electrical Plastic Injection Parts/Mould, as shown inBest Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting.

Fashion Women Hair Accessories Hair Bow Headband for Girls⁰C and left for 2 h ( Tire Repair Strip). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Round Hole Carbon Steel Pipe Perforated for Filter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Professional Manufacturer Stainless Steel Floor Grating. Proteins were also determined by Biuret and Kjeldhal'Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels(400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making). The filtrate in branch B was treated in the same way as branch B inGood Quality PVC Flame Retardant Fabric Tb070. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBrand New Forklift with Double Front pneumatic Tire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ce Quality Double Channel Medical Syringe Pump (THR-SP180D), pH 3.25., mobile phase B: buffer solution of 0.01 mol/SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Factory Sale Various Customized Square 6063 Extruded Aluminum Profile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC) for the financial support.

REFERENCES

Custom Design 100% Silk Tie for Men, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.stamping-mould.com]

Brown, E. M; Thompson, C .J.,Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, M.M.(1994),Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover.The Journal of the American Leather Chemists Association (JALCA)89(7)[M Type Metallic Yarn Lurex Yarn Embroidery Yarn]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Om4 Sc/Upc-LC/Upc Fiber Optic Cable. Analytical Biochemistry212,436-445[FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Nylon Back to Back Hook&Loop. Biotechnology letters 14,6, 531-534[Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes]

Heidemann, E.(1997),Canned Fruit Peach Processing Plant.Leder 48,(5),99-104[Concrete Cure Spray Concrete Curing Agent]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts]

DC Motor for Massagers, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate.Garden Furniture Sofa Rattan Modular Corner Set with Cushions. USP/SP[OEM Eye Anti-Aging Serum]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Water Cream Bulk Storage Tank: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Laminar Air Flow Operating Room]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[900-1800MHz GSM PCB Antenna]

Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer]

Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Neodymium Magnet Pot Assembly with Strong Weight]

Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Seasoned Cast Iron South Africa Three Legged Potjie Pot, Part I.1.0g Water and Air Ozone Generator with Silicone Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Clear PVC Roof Frame Tent in South Africa]

Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),16A/24A High Speed Wire Cable Braiding Machine.Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot, 175-184[Biodegradable T Shirt Bags]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Soundproof Construction Foam PVC/WPC Board]

Whitaker,J.R.(1963),5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050). Analytical Chemistry35(12), 1950-1953[Hot Sale CNC Router Machine for Wood Cutting (1325)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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