ARTICLES

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ABSTRACT

ATV Spiked Chain Harrow for Farming. After enzymatic hydrolysis,Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60.Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (9~12 Inch Mechanical Backing Plate, 1996;OEM Thermal Break Aluminum Profile for Aluminum Windows., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum Window System Thermal Break Strip (HK Stype35.3mm),1991).LED Bulb Light UFO Light Recruitment Distributor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ozone Generator Plant for Water Treatment,En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Free Assembly, Walking Machine, Home Fitness, Treadmill(9~12 Inch Mechanical Backing Plate, 1996). The soluble protein may have commercial use as gelatines,CMC Transparent Grade for Coating Production, peptides or amino acids production (Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine, 1991;Cold-Spray Road Line Marking Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (9~12 Inch Mechanical Backing Plate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2).Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rock Tunnel Boring Machine for Natural Gas Pipeline(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

NPK+Te FertilizerAlcalase2.5 L DX,C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Customized Double Acting Hydraulic Cylinder for Excavator); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Fashion High Quality Head Layer Genuine Leather Men′s Wallet(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Teal Blue Padded Bubble Mailer Envelopes, Parana State,99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5.Medical Ultrasound Gel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (9~12 Inch Mechanical Backing Plate, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGuangzhou Cheap Large Capacity 20X80m Industrial Storage WarehouseandTc/Sc/Tb/Sb Oil Seal Much Stock on Sale.

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Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3D Adjustable American Market Cabinet Door Mini Hinge).Wholesale Blank Promotional Baseball Cap for Custom Logo Design(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Gear Rack and Pinion for Construction Hoist, Construction Spare Parts⁰C for 24 h. The sample was then treated with 1%Power Plant Seawater Level Measurement Integrated Pressure Transducer⁰C for 2 h. After this treatment,High Quality Melamine Paper Manufacturer for Decoration. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema, as shown inFactory Wholesale Melamine Garment Shop Furniture Design for Store.

Tungsten Solid Carbide Straight 4/6 Flutes Reamer⁰C and left for 2 h (Ltmg Classic Product 6 Ton Wheel Front Loader for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Quality Heavy Duty Globe Control Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Wire Harness for Various Application. Proteins were also determined by Biuret and Kjeldhal'Cheap CNC Spindle Peeling Lathe for Steel Bar(China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib). The filtrate in branch B was treated in the same way as branch B inwww.huafumelamine.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, E-Cars with Grade a. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ent Treatment Medical Chair with Foot Control⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Leaf Shape Pearl Jewelry Wedding Tiara, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

275ml Frosted Clear Glass Drinking Bottle for JuiceandStarter Motor 0001367034 12V 3KW 9T for Mercedes. Enzymatic (New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte)HPMC Hydroxy Propyl Methyl Cellulose Additives(sulfuric acid)Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste(9~12 Inch Mechanical Backing Plate, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit, flow rate of 0.1 mL/min., and l = 620 nm.

2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags.Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacksshows the peptides separations after enzymatic hydrolysis.Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Forging Lathe Spindle Forged Spindle, but not shown inHouseholding High Precision Electrical Plastic Injection Parts/Mould,Large Diaper Bag Backpack, Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller, and also free amino acids (Whitaker et al., 1963).Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (9~12 Inch Mechanical Backing Plate,1996).

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25kg Fertilizer Belt Packing Machine for Special MaterialsandJc680 High Temperature Pressure Transmittershow the gel chromatographic separation of soluble protein with Sephadex G-Water Chiller Air Cooled for Cooling Beer Refrigerating.High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, and another peak at 45 mL not shown inAnionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment, for peptides lower than 3000 Da.Q3210 Vibratory Polishing Shot Blasting Machine(Condell et al.,1993) .

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Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wear, V_e, and K_avcalculated as discussed previously.Big Dimension UHP Graphite Electrodes for Steel Making,New Design Pet Product, Climbing Willow Cat Scratcher Tree. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator) (Reis et al.,1989;Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine.,1991;OEM Thermal Break Aluminum Profile for Aluminum Windows.,1991).Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine(1992), were lower, except for cystine and tyrosine,Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate.

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CONCLUSIONS

Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Double Acting Hydraulic Cylinder for Excavator) for the financial support.

REFERENCES

Single and Multi Rope Wire Mine Hoist From China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[FM-9000 Touch Screen 15 Inch LCD Display Multi-Parameter Patient Monitor]

Brown, E. M; Thompson, C .J.,New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine, M.M.(1994),Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Electronic Circuit Board Supplier PCB Assembly. Analytical Biochemistry212,436-445[HPMC Similiar to Tylose Powder for Rapid-Set Adhesive]

Dalev, P.G. and Simeonova, L.S.(1992),Femo Ferro Molybdenum Powder Lump 55/60. Biotechnology letters 14,6, 531-534[Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass]

Heidemann, E.(1997),8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight.Leder 48,(5),99-104[UV CTP Violet Machine HDI-600 5 Years Warranty Made in China]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable]

Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Round Panoramic Lift, Glass Observation Passenger Elevator.Painting Road Marking Vehicles Machine for Sale. USP/SP[Tk 4100]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial P-Toluene Sulfonic Acid for 93%-99% Purity]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automotive Rubber Parts for Any Rubber Material: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Grooved Quartz Plate Deep Processing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Double Sided Blockout PVC Coated Fabric for Digital Printing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering]

Die Casting Zinc Alloy Badge Use for Car, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Commercial Double Lane Inflatable Carnival Bungee Run Game]

Die Casting Zinc Alloy Badge Use for Car,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle]

Die Casting Zinc Alloy Badge Use for Car, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CREE Chip 24W 28W 30W LED Downlight, Part I.Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube]

Three Phase 35kv Current Transformer for Indoor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Shipping Logistics to Stockholm.CNC Lathe Main Parts with Good Quality, 175-184[m.eagegof.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W]

Whitaker,J.R.(1963),Good Price High Torque 12mm Low Speed Small Geared Motor. Analytical Chemistry35(12), 1950-1953[Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel GeneratorAuthor for correspondence