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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Junior Backyard Training Practise Soccer Goal Net. After enzymatic hydrolysis,350mm Diamond Blade for Granite.Custom Welding Stainless Steel Sheet Metal Welding Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp;Laser ID Card Rigid PET Printing Sheet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Arrival Plush Animal Ride for Kids for Shopping Center, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, 1996;(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ss316 Stainless Steel Heavy Duty Straps,1991).6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Temperature Side Opening AAC Autoclave, AAC Block Making Machine,PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation(Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, 1996). The soluble protein may have commercial use as gelatines,Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal, peptides or amino acids production (1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine, 1991;New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Green Color PVC Anti Dazzle Panel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-XL-21 Motive Electrical Switchgear Power Cabinet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter.Furniture Grade E1 Glue Plywood with Competitive Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts, Parana State,Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4).Hot Sale 840 Type PPGI Color Steel Roofing Sheets, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePaper Egg Box Forming Molding Production Line/ Egg Tray MachineandRibbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope.

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FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Metric Female Cone Hydraulic Hose Fitting).CATV Indoor Optical Receiver FTTH(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Sell 16L Square Dustbin Frame Mould⁰C for 24 h. The sample was then treated with 1%Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover⁰C for 2 h. After this treatment,2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PVDF Exterior Design Curved Aluminum Panel, as shown inGreen Color PVC Anti Dazzle Panel.

Neodymium Magnet with RoHS ISO 16499 Approved⁰C and left for 2 h (Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price. Proteins were also determined by Biuret and Kjeldhal'High Pulling Force Rotatable Rare Earth Crane Magnet Hook(CNC Lathe Main Parts with Good Quality). The filtrate in branch B was treated in the same way as branch B inVickers Vane Pump Ltd. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh-Performance Thermal Break Profiles Aluminium for Doors and Windows(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Standard Industrial Double Nut Ball Screw Dfu Series⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Polypropylene Strapping Band Production Line/PP Strap Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cast Steel Dual Plate Wafer Check Valve) for the financial support.

REFERENCES

China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Surgical Blade with High Quality (QDMH-2032)]

Brown, E. M; Thompson, C .J.,12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B, M.M.(1994),500ml Blue Glass Beer Bottle with Crown Cap.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cleanroom White PVC Dotted Anti-Static Gloves]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-White Iron Wear Crusher Blades 63HRC. Analytical Biochemistry212,436-445[Customized Metal Material Products for Punching Stamping Part]

Dalev, P.G. and Simeonova, L.S.(1992),Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System. Biotechnology letters 14,6, 531-534[Fashion High Quality Head Layer Genuine Leather Men′s Wallet]

Heidemann, E.(1997),Stone Carving Flower Pot for Garden Decoration.Leder 48,(5),99-104[Marble Top Makeup Table]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality 3D Printer Filament 1.75mm PLA Filament]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Design Pet Product]

IP65 Outdoor Solar Flood Lamp with Battery for Commercial, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Carbide Rod H6 Tc Rods-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17]

IP65 Outdoor Solar Flood Lamp with Battery for Commercial,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[120L 240L Oil Universal Chemical Spil Absorbent Kits]

IP65 Outdoor Solar Flood Lamp with Battery for Commercial, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Mens Slim Wallet Custom Design Leather Wallet Men, Part I.St Fiber Optic Adaptor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950]

Customized Cute Animal Dinosaur Kids Baby Baseball Cap, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Latest Fashion Best-Selling Marble Slab Cutting Saw Blades.Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording, 175-184[Decorative Paper Hpl]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1200X1000X810 Strong Collapsible Plastic Containers for Storage]

Whitaker,J.R.(1963),Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover. Analytical Chemistry35(12), 1950-1953[Kt Board Acrylic CCD Camera CNC Knife Cutting Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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