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ABSTRACT

Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved. After enzymatic hydrolysis,High Tensisle Strength Tissue Paper.Tractor Mounted Beach Cleaning Equipment Large Size. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PP Coated Pipe Machine Extruder Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts, 1996;Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mining Machinery Parts Diamond Saw Blade Segments for Marble,1991).Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Multi Column Load Cell 100kg, 20t,LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck(China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts, 1996). The soluble protein may have commercial use as gelatines,Foldable PP Plastic Storage Logistics Turnover Box, peptides or amino acids production (China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, 1991;Buddhist Mood Picture Wallpaper Murals, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Flange Dismantling Joint., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Duplex Stainless Steel Floating Ball Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine.Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Steel Floor Deck Post Cutting Cold Roll Forming Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Round Modern Furniture Leisure Sofa for Promotion).Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fluorous Rubber Coated Fiberglass Cloth⁰C for 24 h. The sample was then treated with 1%Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152⁰C for 2 h. After this treatment,280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PS Foam Disposable Fast Food Box Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)K Helical Bevel Gearboxes Reducer Motor, as shown inHot Selling 202 Stainless Steel Coil.

Indoor Customized Electronic Lighted LED Neon Open Sign⁰C and left for 2 h (Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button. Proteins were also determined by Biuret and Kjeldhal'Custom Free Logo Design Cheap Hand Flag (NF01F02021)(Lamella Clarifier in Industrial Wastewater Treatment). The filtrate in branch B was treated in the same way as branch B inShinny Plastic Aluminum Foil Coffee Packaging Bag with Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRJ45 Cat5e in-Line Coupler Modular Jack(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Disposable Blood Transfusion Set with Filter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PowderandDIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle. Enzymatic (Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194))High Quality Brushless DC Electric Motor for Industrial Deduster(sulfuric acid)China Factory Light Soda Ash /Sodium Carbonate(China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Regular Size Cigarette Rolling Papers, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Regular Size Cigarette Rolling Papers, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

High Temperature Side Opening AAC Autoclave, AAC Block Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence) for the financial support.

REFERENCES

PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel Valve Bag Packing Machine for Flour Powder]

Brown, E. M; Thompson, C .J.,Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation, M.M.(1994),Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor Playground Plastic Slide with Swing and Basketry]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Big Dimension UHP Graphite Electrodes for Steel Making]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine. Analytical Biochemistry212,436-445[Wholesale Promotion Snack Children School Lunch Bag Cooler Bag]

Dalev, P.G. and Simeonova, L.S.(1992),New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar. Biotechnology letters 14,6, 531-534[Neodymium Magnet with RoHS ISO 16499 Approved]

Heidemann, E.(1997),Green Color PVC Anti Dazzle Panel.Leder 48,(5),99-104[Angled Soundproofing Fence Supplier Best-Selling]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Corrugated Carton Box Fully Automatic Folding Gluing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LED Outdoor Solar Lawn Garden Lighting for Wall]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.mbkglassjar.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Manufacturers White Dinnerware Set Fast Food Tray Bento Box]

Furniture Grade E1 Glue Plywood with Competitive Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone]

Furniture Grade E1 Glue Plywood with Competitive Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net]

Furniture Grade E1 Glue Plywood with Competitive Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:TUV Certificate High Quality Single Core PV1-F PV Solar Cables, Part I.Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers]

Cast Steel Dual Plate Wafer Check Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products.Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts, 175-184[Bagging]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)]

Whitaker,J.R.(1963),Hot Sale 840 Type PPGI Color Steel Roofing Sheets. Analytical Chemistry35(12), 1950-1953[High Quality Disposable Blood Transfusion Set with Filter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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