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ABSTRACT

China Cube Ice Maker Parts / Ice Machines Maker Cube. After enzymatic hydrolysis,Aluminium/Aluminum Casement Window with Break Bridge (FT-W108).3D Adjustable American Market Cabinet Door Mini Hinge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, 1996;Om4 Sc/Upc-LC/Upc Fiber Optic Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Nylon Back to Back Hook&Loop,1991).Canned Fruit Peach Processing Plant. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate,Garden Furniture Sofa Rattan Modular Corner Set with Cushions,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Water Cream Bulk Storage Tank(Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, 1996). The soluble protein may have commercial use as gelatines,Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR), peptides or amino acids production (Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants, 1991;Seasoned Cast Iron South Africa Three Legged Potjie Pot, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1.0g Water and Air Ozone Generator with Silicone Tube., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-16A/24A High Speed Wire Cable Braiding Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot.5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:20 Inch 48V 750W Fat Tire Electric Bike with Double Seat(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3D Creative Design Metal Medal Promotion Gift of Sport(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Stainless Steel Laser Cutting).Ad-L13 Black Perfume Plastics Vial Bottle 10ml(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover⁰C for 24 h. The sample was then treated with 1%EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)⁰C for 2 h. After this treatment,60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Universal Mirco Forklift Car Bus Truck Seats Switch Sensors's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder, as shown inHousehold Family Medical Emergency Electronic Digital Thermometer.

Mexico Single Expansion Anchor with YZP⁰C and left for 2 h (Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet. Proteins were also determined by Biuret and Kjeldhal'Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope(Boat Aluminum Pipe 5083 Marine Used Aluminum Tube). The filtrate in branch B was treated in the same way as branch B inSMD Type 1.5mm Pitch Wire to Board Connector. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Whole Absorbtion Flexo Printing Soltting Die Cutting Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Price 4X6 Acrylic Magnetic Photo Frame. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av200m Diesel Oil Hydraulic Water Well Drilling Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Air Purification Activated Alumina⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Resistan UV Grades PP Non Woven Fabric, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Outdoor Fitness Equipment Swing (JMH-03).Multi Sensor Nilitary Surveillance Thermal Imaging Security camerashows the peptides separations after enzymatic hydrolysis.Sheet Metal Distribution Cabinet with High Quality(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Cube Ice Maker Parts / Ice Machines Maker Cube, but not shown inElectroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB,OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk, and also free amino acids (Whitaker et al., 1963).New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover,1996).

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CONCLUSIONS

SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Small Boat Deck Knuckle Boom Ship Pedestal Crane) for the financial support.

REFERENCES

Line Scan Uvss System Portable Under Vehicle Surveillance, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Large Frameless Mirror]

Brown, E. M; Thompson, C .J.,Folding Steel Manual Wheelchair with Chrome Frame, M.M.(1994),Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Road Construction Non Woven Needle Punched PP Non Woven Geotextile]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Style Lady Alloy Quartz Watch with Simple Dial. Analytical Biochemistry212,436-445[High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic]

Dalev, P.G. and Simeonova, L.S.(1992),Rooftop HVAC Packaged Units (RTU) Amrta Manufacture. Biotechnology letters 14,6, 531-534[custom design MIFARE DESFire EV2 2K plastic RFID loyalty card]

Heidemann, E.(1997),Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box.Leder 48,(5),99-104[Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Diesel Pipe Ss Thread Fitting Flange Cover]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dark Green Decorative Garden Welded Wire Mesh Fencing]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[8011 Aluminum Foil for Cap Used]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Garden Furniture Sofa Rattan Modular Corner Set with Cushions]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Plush Baby Sofa Chair]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[DC Motor for Massagers]

Factory Supply Straight Ball Joint Bearing 02b04, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wrinkle Effect Crease UV Drying Machine UV Curing System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Single Core Solid Copper Conductor Rg Coaxial Cable]

Factory Supply Straight Ball Joint Bearing 02b04,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Hose Fittings]

Factory Supply Straight Ball Joint Bearing 02b04, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack, Part I.Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Printed Socks]

20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tefude Feeding and Packaging Line for Food with Tray.Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, 175-184[Dual Usb Charger Fast]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30]

Whitaker,J.R.(1963),Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine. Analytical Chemistry35(12), 1950-1953[World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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