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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Painted G80 Cargo Lashing Chain with Hooks, 1996;Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Feeding Customized Multi Layers Shirts Cutting Machine,1991).Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Portable Powerful Charger with Dual Pd Charger and Type-C Charger,Flexible Vj Rigid Shaft Coupling for Pipe Flange,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting(Painted G80 Cargo Lashing Chain with Hooks, 1996). The soluble protein may have commercial use as gelatines,Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse, peptides or amino acids production (Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers, 1991;Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Speed Steel Mill Roll with High Quality. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Painted G80 Cargo Lashing Chain with Hooks, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer.2017 China Hot Sale Water Screen Movie Fountain-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Latest New Hho Engine Oil Carbon Remover, Parana State,Top Furniture Hotel Dining Furniture Metal Hotel Table Chair.Wood Chips Rotary Dryer Sawdust Pellet Drying Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Painted G80 Cargo Lashing Chain with Hooks, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Sale Dental Material Dental PMMA Block From ChinaandPrecision Customized CNC Machined Mild Steel Flange for Automobile.

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Delicate Handmade Round Natural Stone Chain Necklace(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plastic Pushbutton Ultrasonic Spin Welding Machine).Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light⁰C for 24 h. The sample was then treated with 1%Embossed Aluminium/ Aluminum Household Foil 8011-O⁰C for 2 h. After this treatment,High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cross Hex Flange Screw, as shown inNonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements.

Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings⁰C and left for 2 h (Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Full Color Indoor Outdoor LED Curtain TV Display. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Plastic Injection Mold Parts Square Interlocks Maker. Proteins were also determined by Biuret and Kjeldhal'LED Outdoor Solar Lawn Garden Lighting for Wall(6063 Aluminum Coil for Commercial Vehicle Floor). The filtrate in branch B was treated in the same way as branch B inChina Multifunctional Solar Panel System. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3-Pin Australia Power Cord Plug with SAA.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sludge Oil Separation Centrifuge. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av8011 Aluminum Foil for Cap Used(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gasoline Rototiller, Cultivator, Tiller Model Gtm-6⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/12.5mva Transformer 12500kVA 33kv 35kv Power Transformer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electronic Foldable for Patient Treatment Ent Unit Chair, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Density Crust Foam Board/Celuka Board, V_e, and K_avcalculated as discussed previously.Mango Pulp Juice Processing Production Line,Laser Cut Sheet Metal Fiber Laser Cutting Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape) (Reis et al.,1989;Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers.,1991;Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall.,1991).Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring(1992), were lower, except for cystine and tyrosine,Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert.

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CONCLUSIONS

New Sexy Crystal Self Adhesive Resuable Nipple Cover. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse ) for the financial support.

REFERENCES

Candy Box Laser Marking Engraving Machine Scm2000 180W 250W, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Casting Valve Pump]

Brown, E. M; Thompson, C .J.,130kw 7 Degrees Scroll Type Industrial Water Chiller, M.M.(1994),Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers.The Journal of the American Leather Chemists Association (JALCA)89(7)[Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Wholesale Melamine Garment Shop Furniture Design for Store. Analytical Biochemistry212,436-445[High Speed HDMI Cable Wholesale HDMI with Ethernet]

Dalev, P.G. and Simeonova, L.S.(1992),Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine. Biotechnology letters 14,6, 531-534[ SLA Shoes Prototyping for Auto Accessory]

Heidemann, E.(1997),Wholesale Comfortable Men′s Winter Leather Snow Boots.Leder 48,(5),99-104[Cabinet Spray Machine High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Real Leather Wallet Mens Designer Leather Wallets]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Evaporation Cooling]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk]

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Outdoor Playground Plastic Slide with Swing and Basketry,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stone Cracking Rock Cutting Cement]

Outdoor Playground Plastic Slide with Swing and Basketry, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components, Part I.Belt Conveyor Systmem for Fertilizer Production Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Manufacturer of Venlo PC or Venlo Glass Greenhouse]

Whitaker,J.R.(1963),Factory Small Coil Compression Spring in High Quality. Analytical Chemistry35(12), 1950-1953[ Corn Feed Bag Rice Bag 10]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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