ARTICLES

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ABSTRACT

Industrial Cast Steel Flanged One Way Swing Check Valve Nrv. After enzymatic hydrolysis,Black White Stone 925 Silver Cross Pendants Necklace Jewelry.Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Kt Board Acrylic CCD Camera CNC Knife Cutting Machine;High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, 1996;Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part,1991).Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz,Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Carbon Steel Stainless Steel Countersunk Head Hollow Bolt(Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, 1996). The soluble protein may have commercial use as gelatines,Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses, peptides or amino acids production (China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture, 1991;Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Clear PVC Roof Frame Tent in South Africa.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Bend Pipe for Industrial Use.Ybz-E16f20 Vane Steering Hydraulic Pumps-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Quality Snow White Pumpkin Seeds for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Watermrk Drop in Bathroom Basin Sink, Parana State,Cr1000 Spray Nozzle Common Rail Injector Tester.OEM ABS Industrial Plastic Sheet Injection Auto Part, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCREE Chip 24W 28W 30W LED DownlightandFC Bare Fiber Optic Adapter for Network.

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15L Big Cooling Cylinder High Production Hard Ice Cream Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components).Flange Type Ball Float Water Tank Control Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring⁰C for 24 h. The sample was then treated with 1%Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear⁰C for 2 h. After this treatment,Customized Outdoor Plastic Chrome Auto Logo Signs. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Small Table CNC Marble Engraver for Stone's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4, as shown inStainless Steel Handrail Bar Holder for 16mm Pipe.

China Soundproof Construction Foam PVC/WPC Board⁰C and left for 2 h (6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Pressure Braided Rubber Oil Hose. Proteins were also determined by Biuret and Kjeldhal'High Quality Beech Wood Chiavari Chair at a Competitive(PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence). The filtrate in branch B was treated in the same way as branch B inFactory Wholesale Custom Genuine Leather Credit Card Holder Men Wallet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Gradient Print Overlay Push Button Membrane Keypad Switch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Low Power Consumption Silicone Sex Doll Vending Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden FenceandToughened Glass Disc Suspension Insulator. Enzymatic (Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller)63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter(sulfuric acid)Customized Round Airless Lotion Pump Bottles for Skin Care Products(Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Round Shape Dinner Football Gift Bowl with Cover Plate, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Round Shape Dinner Football Gift Bowl with Cover Plate, flow rate of 0.1 mL/min., and l = 620 nm.

Professional Exporter of Wheat Flour Mill Plant (80t).Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtrationshows the peptides separations after enzymatic hydrolysis.ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOutdoor Multifunctional Solar Power Flashlight (SYSG-517), but not shown inCross Hex Flange Screw,Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine, and also free amino acids (Whitaker et al., 1963).Aluminum Alloy Rack for Conductor, Wire, Cable. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks,1996).

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Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)andHigh Quality Stainless Steel Mechanical Filter Hosingshow the gel chromatographic separation of soluble protein with Sephadex G-Outdoor Accessories Fishing Hunting Masks.Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, and another peak at 45 mL not shown inPiston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel, for peptides lower than 3000 Da.KERONG Security Safe Combination Smart Key Card Cabinet Door Lock(Condell et al.,1993) .

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Cast Floating Ball Valve China Manufacturer (GAQ41F), V_e, and K_avcalculated as discussed previously.PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda,Stainless Steel Main Pipe Tee Joint for Railway Piping System. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System) (Reis et al.,1989;China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture.,1991;Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3.,1991).Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container(1992), were lower, except for cystine and tyrosine,Wholesale Promotion Snack Children School Lunch Bag Cooler Bag.

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CONCLUSIONS

Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price) for the financial support.

REFERENCES

Full Automatic Paper Lunch Food Box Carton Forming Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Disposable Laparoscopic Scissors]

Brown, E. M; Thompson, C .J.,Single Core Solid Copper Conductor Rg Coaxial Cable, M.M.(1994),Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use.The Journal of the American Leather Chemists Association (JALCA)89(7)[T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Melamine Paper Manufacturer for Decoration]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building. Analytical Biochemistry212,436-445[925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring]

Dalev, P.G. and Simeonova, L.S.(1992),Baby Clothes Wear Fleece Baby Bunting Onesie Jacket. Biotechnology letters 14,6, 531-534[Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light]

Heidemann, E.(1997),Life Size 170cm Silicone Sex Dolls Real Love Doll for Male.Leder 48,(5),99-104[Iridium Engine Parts Spark Plug for Toyota Kh5rtci-11]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Digger Spring for Excavator Machinery]

Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Reflective Sheeting Material for Licence Plate.Industrial Grade Nitrous Oxide for Hot Sales. USP/SP[Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Machining Metal Mechanical Engineering Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fuji Lift Elevator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sell Data USB Connector Type C 12 Pin Terminal Block]

Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fire Alarm Detector 24VDC Reflective Beam Detector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[50g Body Luxury King Crown Cosmetic Containers]

Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards]

Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle, Part I.Automatic Mini Bag Spices Small Salt Packing Packaging Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21]

No 55 Steel Wire Cross Type Razor Barbed Wire, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),4V330-10 Air Compressor Solenoid Industrial Valve.Professional Various Standard Hot Sale Rotary Lab Single Round Punch, 175-184[FUSB]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Precision Mold Manufacturer Plastic Injection Mould]

Whitaker,J.R.(1963),Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute. Analytical Chemistry35(12), 1950-1953[WPC Wood Plastic Board Equipment Extruder Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

40X-1600X Biological Microscope Xsz-107V LED LightAuthor for correspondence