ARTICLES

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ABSTRACT

100% Latex Free Endotracheal Tube with PVC Material. After enzymatic hydrolysis,Neodymium Magnet Pot Assembly with Strong Weight.Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts, 1996;CH Series Pid Intelligent Temperature Controller CH102., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Householding High Precision Electrical Plastic Injection Parts/Mould,1991).Fashion Women Hair Accessories Hair Bow Headband for Girls. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Round Hole Carbon Steel Pipe Perforated for Filter,Professional Manufacturer Stainless Steel Floor Grating,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels(Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts, 1996). The soluble protein may have commercial use as gelatines,Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose, peptides or amino acids production (Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips, 1991;Brand New Forklift with Double Front pneumatic Tire, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ce Quality Double Channel Medical Syringe Pump (THR-SP180D). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity.High Quality Rubber Pressed Mixing Machine with Ce Certification-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can, Parana State,Mini Pencil Tag with Lanyard (YS211).Practical Edible Silicone Kitchenware (YB-ty-29), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWaterproof PVDF Aluminum Curtain Wall PanelandIntelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.

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4 Inch Quarter Round PCD Diamond Grinding Cup Wheel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Styles Fashion Erotic Women Underwear Sexy Lingerie).High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)⁰C for 24 h. The sample was then treated with 1%Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl⁰C for 2 h. After this treatment,99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Cube Ice Maker Parts / Ice Machines Maker Cube, as shown in1.0mm Binding Wire Galvanized Iron Wire.

Aluminium/Aluminum Casement Window with Break Bridge (FT-W108)⁰C and left for 2 h (Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3D Adjustable American Market Cabinet Door Mini Hinge. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Sale Various Customized Square 6063 Extruded Aluminum Profile. Proteins were also determined by Biuret and Kjeldhal'Custom Design 100% Silk Tie for Men(115mm Segmented Saw Blade for Concrete Cutting). The filtrate in branch B was treated in the same way as branch B inFactory Price Custom Rubber Bracelets Silicone Wristband for Event. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOm4 Sc/Upc-LC/Upc Fiber Optic Cable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Canned Fruit Peach Processing Plant⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Garden Furniture Sofa Rattan Modular Corner Set with Cushions, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine.Fluorous Rubber Coated Fiberglass Clothshows the peptides separations after enzymatic hydrolysis.16A/24A High Speed Wire Cable Braiding Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEfficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine, but not shown inBuilding Wall Machine for Hot Machine,Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot, and also free amino acids (Whitaker et al., 1963).5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts,1996).

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Small Boat Deck Knuckle Boom Ship Pedestal Crane, V_e, and K_avcalculated as discussed previously.115mm Segmented Saw Blade for Concrete Cutting,Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle) (Reis et al.,1989;Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips.,1991;CH Series Pid Intelligent Temperature Controller CH102.,1991).China Round Transparent Glass 750ml Liquor Glass Bottle(1992), were lower, except for cystine and tyrosine,3D Creative Design Metal Medal Promotion Gift of Sport.

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CONCLUSIONS

Ad-L13 Black Perfume Plastics Vial Bottle 10ml. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)) for the financial support.

REFERENCES

Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bsp Female Thread Forged Hydraulic Hose Fitting]

Brown, E. M; Thompson, C .J.,EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012), M.M.(1994),60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Universal Mirco Forklift Car Bus Truck Seats Switch Sensors. Analytical Biochemistry212,436-445[Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo]

Dalev, P.G. and Simeonova, L.S.(1992),Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder. Biotechnology letters 14,6, 531-534[Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated]

Heidemann, E.(1997),Mexico Single Expansion Anchor with YZP.Leder 48,(5),99-104[XL-21 Type OEM Motive Motive Electrical Switchgear Power Cabinet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Colorful Braided Fabric Cable Round]

Powder Coating Paint Lines Systems for Factory /Plants, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe.SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet. USP/SP[T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rotor High Output Electric 120 Watt Motor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5 Star European Antique Hotel Furniture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Abb Industrial Plug And Socket]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings]

Whole Absorbtion Flexo Printing Soltting Die Cutting Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Price 4X6 Acrylic Magnetic Photo Frame-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6)]

Whole Absorbtion Flexo Printing Soltting Die Cutting Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover]

Whole Absorbtion Flexo Printing Soltting Die Cutting Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:200m Diesel Oil Hydraulic Water Well Drilling Machine, Part I.Custom Cmyk Printing Cake Gift Packing Box with Lid. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Salon Equipment Hair Clipper]

Air Purification Activated Alumina, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6).Resistan UV Grades PP Non Woven Fabric, 175-184[Carbon Steel Thread Rod]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2017 China Hot Sale Water Screen Movie Fountain]

Whitaker,J.R.(1963),New Scba Breathing Apparatus Equipment with Ce Certification. Analytical Chemistry35(12), 1950-1953[Expanded Metal Infill Panel Metal Mesh Custom Made]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow TruckAuthor for correspondence