ARTICLES

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ABSTRACT

Mining Machinery Parts Diamond Saw Blade Segments for Marble. After enzymatic hydrolysis,Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface.Multi Column Load Cell 100kg, 20t. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System;Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Foldable PP Plastic Storage Logistics Turnover Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, 1996;Buddhist Mood Picture Wallpaper Murals., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Flange Dismantling Joint,1991).Duplex Stainless Steel Floating Ball Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card,Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple(China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, 1996). The soluble protein may have commercial use as gelatines,Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, peptides or amino acids production (CNC Machine Aluminum Telescopic Covers Flat Bellows Cover, 1991;7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Kubota Combine Harvester Spare Parts 5h484-2392 Nut.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor.Steel Floor Deck Post Cutting Cold Roll Forming Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

K Helical Bevel Gearboxes Reducer Motor, Parana State,Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances.Indoor Customized Electronic Lighted LED Neon Open Sign, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Cardand2D LED Rope Lighting Motif Lights for Christmas Decoration.

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Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016).Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Free Logo Design Cheap Hand Flag (NF01F02021)⁰C for 24 h. The sample was then treated with 1%125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card⁰C for 2 h. After this treatment,Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)RJ45 Cat5e in-Line Coupler Modular Jack's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture, as shown inMetal Cuting CNC Plasma Cutting Machine (DW1325).

Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper⁰C and left for 2 h (Automatic Mini Bag Spices Small Salt Packing Packaging Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Disposable Blood Transfusion Set with Filter. Proteins were also determined by Biuret and Kjeldhal'DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle(SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel). The filtrate in branch B was treated in the same way as branch B inPet PE BOPP PVC CPP Film Slitting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Brushless DC Electric Motor for Industrial Deduster. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Factory Light Soda Ash /Sodium Carbonate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.304L Stainless Steel U Channel Good Performance⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Junior Backyard Training Practise Soccer Goal Net, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniformand350mm Diamond Blade for Granite. Enzymatic (Custom Welding Stainless Steel Sheet Metal Welding Parts)Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp(sulfuric acid)Laser ID Card Rigid PET Printing Sheet(China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Arrival Plush Animal Ride for Kids for Shopping Center, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Arrival Plush Animal Ride for Kids for Shopping Center, flow rate of 0.1 mL/min., and l = 620 nm.

Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine.Deposited Stainless Steel Rivet Nut with Knurledshows the peptides separations after enzymatic hydrolysis.(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inResistan UV Grades PP Non Woven Fabric, but not shown inFabric Coated PVC for Garment Woven Fabric 600d 100% Polyester,Ss316 Stainless Steel Heavy Duty Straps, and also free amino acids (Whitaker et al., 1963).6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005,1996).

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Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal, V_e, and K_avcalculated as discussed previously.1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine,New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System) (Reis et al.,1989;CNC Machine Aluminum Telescopic Covers Flat Bellows Cover.,1991;Buddhist Mood Picture Wallpaper Murals.,1991).Green Color PVC Anti Dazzle Panel(1992), were lower, except for cystine and tyrosine,ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator.

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CONCLUSIONS

XL-21 Motive Electrical Switchgear Power Cabinet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)) for the financial support.

REFERENCES

Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Metal Thread Porous Filter]

Brown, E. M; Thompson, C .J.,Furniture Grade E1 Glue Plywood with Competitive Price, M.M.(1994),High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[115mm Segmented Saw Blade for Concrete Cutting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-TUV Certificate High Quality Single Core PV1-F PV Solar Cables. Analytical Biochemistry212,436-445[OEM Factory New Girls Nuts Crunch Cheap Price]

Dalev, P.G. and Simeonova, L.S.(1992),Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners. Biotechnology letters 14,6, 531-534[Fluorous Rubber Coated Fiberglass Cloth]

Heidemann, E.(1997),Cast Steel Dual Plate Wafer Check Valve.Leder 48,(5),99-104[Gc-1032 Under Ground Metal Detector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine]

PP Coated Pipe Machine Extruder Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products.Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts. USP/SP[Building Wall Machine for Hot Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Price Graphite Electrode HP 300mm UHP]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale 840 Type PPGI Color Steel Roofing Sheets: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Voltage Transformer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Desktop Single Wave Soldering Machine/Wave Soldering Machine]

FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CATV Indoor Optical Receiver FTTH-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes]

FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Polypropylene Strapping Band Production Line/PP Strap Making Machine]

FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Sell 16L Square Dustbin Frame Mould, Part I.Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml.PVDF Exterior Design Curved Aluminum Panel, 175-184[febwebbing.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Furniture Grade E1 Glue Plywood with Competitive Price]

Whitaker,J.R.(1963),Neodymium Magnet with RoHS ISO 16499 Approved. Analytical Chemistry35(12), 1950-1953[High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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