ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Animal Carcass Incinerator Hospital Garbage Incinerator. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material

ABSTRACT

High Density Crust Foam Board/Celuka Board. After enzymatic hydrolysis,Mango Pulp Juice Processing Production Line.Laser Cut Sheet Metal Fiber Laser Cutting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring;Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Sexy Crystal Self Adhesive Resuable Nipple Cover, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Candy Box Laser Marking Engraving Machine Scm2000 180W 250W, 1996;130kw 7 Degrees Scroll Type Industrial Water Chiller., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers,1991).Factory Wholesale Melamine Garment Shop Furniture Design for Store. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine,Wholesale Comfortable Men′s Winter Leather Snow Boots,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality(Candy Box Laser Marking Engraving Machine Scm2000 180W 250W, 1996). The soluble protein may have commercial use as gelatines,Gcc Plant Ball Mill and Air Classifier Production Line, peptides or amino acids production (Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes, 1991;Outdoor Playground Plastic Slide with Swing and Basketry, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Belt Conveyor Systmem for Fertilizer Production Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Candy Box Laser Marking Engraving Machine Scm2000 180W 250W, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price.Digger Spring for Excavator Machinery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1.8ml Clear Sample Vials with Label and PP Caps(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Brand New Forklift with Double Front pneumatic Tire).ATV Spiked Chain Harrow for Farming(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60⁰C for 24 h. The sample was then treated with 1%Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody⁰C for 2 h. After this treatment,Fashionable Natural Bamboo Wood Sunglasses for Men. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Solar Powered Outdoor Lighting LED Solar Wall Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs, as shown inStainless Steel Furniture Gold PVD Plasma Coating Machine.

9~12 Inch Mechanical Backing Plate⁰C and left for 2 h (High Quality Mens Slim Wallet Custom Design Leather Wallet Men). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM Thermal Break Aluminum Profile for Aluminum Windows. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aluminum Window System Thermal Break Strip (HK Stype35.3mm). Proteins were also determined by Biuret and Kjeldhal'LED Bulb Light UFO Light Recruitment Distributor(Commercial Inflatable Dry Slide for Kids). The filtrate in branch B was treated in the same way as branch B inTrunk Lid Mobile Antenna Mount. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ozone Generator Plant for Water Treatment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFree Assembly, Walking Machine, Home Fitness, Treadmill(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cold-Spray Road Line Marking Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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NPK+Te Fertilizer. Melamine/Plain Chipboardshows the peptides separations after enzymatic hydrolysis.C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTtp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, but not shown inPainted G80 Cargo Lashing Chain with Hooks,Customized Double Acting Hydraulic Cylinder for Excavator, and also free amino acids (Whitaker et al., 1963).Fashion High Quality Head Layer Genuine Leather Men′s Wallet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Candy Box Laser Marking Engraving Machine Scm2000 180W 250W,1996).

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Wholesale Blank Promotional Baseball Cap for Custom Logo Design, V_e, and K_avcalculated as discussed previously.Gear Rack and Pinion for Construction Hoist, Construction Spare Parts,Power Plant Seawater Level Measurement Integrated Pressure Transducer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( 49) (Reis et al.,1989;Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes.,1991;130kw 7 Degrees Scroll Type Industrial Water Chiller.,1991).High Quality Melamine Paper Manufacturer for Decoration(1992), were lower, except for cystine and tyrosine,2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese.

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CONCLUSIONS

Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel) for the financial support.

REFERENCES

Tungsten Solid Carbide Straight 4/6 Flutes Reamer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Chicken Raising Equipment]

Brown, E. M; Thompson, C .J.,China Quality Heavy Duty Globe Control Valve, M.M.(1994),Customized Wire Harness for Various Application.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Factory Light Soda Ash /Sodium Carbonate]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cheap CNC Spindle Peeling Lathe for Steel Bar. Analytical Biochemistry212,436-445[Csm DIY Microscope for Kids/Toy Microscope]

Dalev, P.G. and Simeonova, L.S.(1992),Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements. Biotechnology letters 14,6, 531-534[Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)]

Heidemann, E.(1997),3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, E-Cars with Grade a.Leder 48,(5),99-104[New Design Men Price Casual Men Casual Shoes]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Electronic Circuit Board Supplier PCB Assembly]

Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China.Sc LC FC St Fiber Optical Adaptor Coupler Adapter. USP/SP[70L Lab Use Refrigerated Circulator Chiller Factory Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Duplex Stainless Steel Floating Ball Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ent Treatment Medical Chair with Foot Control: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Flexible Vj Rigid Shaft Coupling for Pipe Flange]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Tinted Glass]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents]

Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Leaf Shape Pearl Jewelry Wedding Tiara-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mashroom Head Nylon Anchor with Pozi Nail]

Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Muliti-Size Light Hot Stamping Foil for PS Frame]

Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Starter Motor 0001367034 12V 3KW 9T for Mercedes, Part I.New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price]

HPMC Hydroxy Propyl Methyl Cellulose Additives, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste.Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit, 175-184[808nm Hair Removal Laser Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS]

Whitaker,J.R.(1963),2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags. Analytical Chemistry35(12), 1950-1953[ 6063]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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