ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Real Leather Wallet Mens Designer Leather Wallets. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)

ABSTRACT

Full Port Small Automatic Electronic Plumbing Ball Valve. After enzymatic hydrolysis,Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,.Cleanroom White PVC Dotted Anti-Static Gloves. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail;Marine Solas Adhesive Reflective Tape; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, 1996;ASTM A240 304 316L No. 1 Stainless Steel Sheet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (12V 24V 220V Digital Temperature Controller,1991).Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,25kg Fertilizer Belt Packing Machine for Special Materials,Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rotor High Output Electric 120 Watt Motor(Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, 1996). The soluble protein may have commercial use as gelatines,Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, peptides or amino acids production (Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed, 1991;Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rpl Straight Socket and Plugs Male Thread.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Metal Stamping Stainless Steel Parts with CNC Machining.Ss Wafer Type Check Valve Dn32 Dn40 Dn50-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CNC Produce Medical Machining Custom Metal Milling(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ss316 Stainless Steel Heavy Duty Straps).Multi Sensor Nilitary Surveillance Thermal Imaging Security camera(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Metal Stamping Welding Spare Parts for Farming⁰C for 24 h. The sample was then treated with 1%Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine⁰C for 2 h. After this treatment,Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)275ml Frosted Clear Glass Drinking Bottle for Juice's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price, as shown inVegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper.

Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs⁰C and left for 2 h (USB LED Clock Fan USB-Powered Portable Fan with Clock). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bolts and Nuts Set Brass Fasteners Double Hex Bolt. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Direct Sales Kids Boy Cotton Children T Shirt. Proteins were also determined by Biuret and Kjeldhal'Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener(Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)). The filtrate in branch B was treated in the same way as branch B inFood Additive Sodium Tripolyphosphate (STPP) Food Grade. The final soluble protein shown were used for peptides separations using gel permeation chromatography,15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Light Blue PVC Plastic Sheet and PVC Film. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Dental Material Dental PMMA Block From China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Waste Pet Flakes Recycling Washing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Deposited Stainless Steel Rivet Nut with Knurled, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

500W 1000W Stainless Steel Copper Fiber Metal Cutting EquipmentandHigh Quality Stainless Steel Mechanical Filter Hosing. Enzymatic (Paper New Year Greeting Cards Wedding Invitations Cards)Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01)(sulfuric acid)BLE5.0 Ttl to Bluetooth Module with at Command(Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle, flow rate of 0.1 mL/min., and l = 620 nm.

Professional Customization Screen Printing Machine for Porous Ceramic.13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment Systemshows the peptides separations after enzymatic hydrolysis.Fea040b New Arrival Airplane Seat Belt Extender(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLongyi Crimping Types Cable Terminal Lugs, but not shown inFluorine Lined Butterfly Valve Flanged Type Anti Corrosion,SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, and also free amino acids (Whitaker et al., 1963).Square Shape Glass Candle Holder for One Piece Tealight Candle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers,1996).

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Easy Foldable Kids Walking Chair Toys Baby Walker, V_e, and K_avcalculated as discussed previously.Men V-Neck Longline T-Shirt with Color Block Ribbed Trims,Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors) (Reis et al.,1989;Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed.,1991;ASTM A240 304 316L No. 1 Stainless Steel Sheet.,1991).Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve(1992), were lower, except for cystine and tyrosine,Outdoor Multifunctional Solar Power Flashlight (SYSG-517).

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CONCLUSIONS

Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (WPC Wood Plastic Board Equipment Extruder Machine) for the financial support.

REFERENCES

Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Outdoor Inverter Enclosure Extruded Aluminum Enclosure]

Brown, E. M; Thompson, C .J.,High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer, M.M.(1994),FC Bare Fiber Optic Adapter for Network.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ent Treatment Medical Chair with Foot Control]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Noiseless) Cracking Agent (SCA) for Breaking Rocks]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory. Analytical Biochemistry212,436-445[Factory Direct Sales Kids Boy Cotton Children T Shirt]

Dalev, P.G. and Simeonova, L.S.(1992),Cattle Corpse Cremation Burning Stove. Biotechnology letters 14,6, 531-534[High-Performance Thermal Break Profiles Aluminium for Doors and Windows]

Heidemann, E.(1997),L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale.Leder 48,(5),99-104[Sheet-Metal Housings Part in Small Batches]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Compost Turner Machine]

Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge.2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment. USP/SP[Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Latest Fashion Best-Selling Marble Slab Cutting Saw Blades]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Stainless Steel Biological Fermentor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Boshi 38mm Shackle Aluminum Safety Padlock (BD-A20)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Iron Wire Mesh Chain Link Mesh Security Fence]

16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rj11, Rj12, RJ45 Network Cable Modular Plug Crimping Tool Lk-Nt005-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Brushless DC Electric Motor for Industrial Deduster]

16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment]

16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing, The Assembly, The Packaging,and End of Life for The Mould and Mould Part, Part I.16 Spindle Lace High Speed Braiding Machine Textile Machinery. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses]

Micro Button Type Load Cell Compression Load Cell 10kg, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building.Interlocking Precast Plastic Concrete Paver Molds for Sale, 175-184[Hot Sale Custom Shape Name Blank Metal Necklace Pendant Sublimation Dog Tag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Roller Hearth Heating Furnace for Gas Cylindrer]

Whitaker,J.R.(1963),24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum. Analytical Chemistry35(12), 1950-1953[CMP No Nc Metal Switch Black 25mm Illuminated Push Button]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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