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ABSTRACT

Rock Tunnel Boring Machine for Natural Gas Pipeline. After enzymatic hydrolysis,NPK+Te Fertilizer.C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Teal Blue Padded Bubble Mailer Envelopes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Medical Ultrasound Gel, 1996;Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Blank Promotional Baseball Cap for Custom Logo Design,1991).Gear Rack and Pinion for Construction Hoist, Construction Spare Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Power Plant Seawater Level Measurement Integrated Pressure Transducer,High Quality Melamine Paper Manufacturer for Decoration,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese(Medical Ultrasound Gel, 1996). The soluble protein may have commercial use as gelatines,Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema, peptides or amino acids production (Tungsten Solid Carbide Straight 4/6 Flutes Reamer, 1991;China Quality Heavy Duty Globe Control Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Wire Harness for Various Application., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cheap CNC Spindle Peeling Lathe for Steel Bar.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Medical Ultrasound Gel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, E-Cars with Grade a.6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sc LC FC St Fiber Optical Adaptor Coupler Adapter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Road Construction Non Woven Needle Punched PP Non Woven Geotextile).Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags⁰C for 24 h. The sample was then treated with 1%Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging⁰C for 2 h. After this treatment,Large Diaper Bag Backpack, Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Water Chiller Air Cooled for Cooling Beer Refrigerating, as shown inJcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700.

High Quality Cheap Price Disposable IV Infusion Set with Precise Filter⁰C and left for 2 h (High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Q3210 Vibratory Polishing Shot Blasting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wear. Proteins were also determined by Biuret and Kjeldhal'Big Dimension UHP Graphite Electrodes for Steel Making(custom design MIFARE DESFire EV2 2K plastic RFID loyalty card). The filtrate in branch B was treated in the same way as branch B inStainless Steel Wire Rope 8mm. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New Design Pet Product, Climbing Willow Cat Scratcher Tree.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Single and Multi Rope Wire Mine Hoist From China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automotive Rubber Parts for Any Rubber Material.Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hoseshows the peptides separations after enzymatic hydrolysis.Die Casting Zinc Alloy Badge Use for Car(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in8011 Aluminum Foil for Cap Used, but not shown inGarden Furniture Sofa Rattan Modular Corner Set with Cushions,Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge, and also free amino acids (Whitaker et al., 1963).CREE Chip 24W 28W 30W LED Downlight. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Medical Ultrasound Gel,1996).

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CONCLUSIONS

Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Leaf Shape Pearl Jewelry Wedding Tiara) for the financial support.

REFERENCES

Mashroom Head Nylon Anchor with Pozi Nail, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[White Color Silage Wrap Film Silage Packing Machine Use Silage Stretch Film]

Brown, E. M; Thompson, C .J.,Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool, M.M.(1994),Waterjet 87K Intensifier Part Check Valve Body 020077-1.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Diatomite Earth Beer Filter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Meat Machinery/Chicken Breast and Fish Slicing Machine. Analytical Biochemistry212,436-445[New Scba Breathing Apparatus Equipment with Ce Certification]

Dalev, P.G. and Simeonova, L.S.(1992),Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine. Biotechnology letters 14,6, 531-534[Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesales Stackable Coffee Iron Chair]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Spark Resistant Construction Axial Fan with Fixed Pitch Blade]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Eye Anti-Aging Serum: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Sell 16L Square Dustbin Frame Mould]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100kVA Electric Cummins Powered Silent Diesel Generator with Ce/ISO]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine]

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Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken]

Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Microfiber Towel Car Drying Soft Towel, Part I.Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl]

High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food.100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine, 175-184[Double Sigma Mixer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PVDF Exterior Design Curved Aluminum Panel]

Whitaker,J.R.(1963),New Styles Fashion Erotic Women Underwear Sexy Lingerie. Analytical Chemistry35(12), 1950-1953[Mini Succulent Planters]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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