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ABSTRACT

Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse. After enzymatic hydrolysis,Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers.Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Speed Steel Mill Roll with High Quality, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer, 1996;2017 China Hot Sale Water Screen Movie Fountain., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes,1991).Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip,Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse ,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale(3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer, 1996). The soluble protein may have commercial use as gelatines,Latest New Hho Engine Oil Carbon Remover, peptides or amino acids production (Wood Chips Rotary Dryer Sawdust Pellet Drying Machine, 1991;Delicate Handmade Round Natural Stone Chain Necklace, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Embossed Aluminium/ Aluminum Household Foil 8011-O. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021).Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cross Hex Flange Screw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3-Pin Australia Power Cord Plug with SAA, Parana State,Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting.Sludge Oil Separation Centrifuge, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCast Steel Dual Plate Wafer Check ValveandButterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve.

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8011 Aluminum Foil for Cap Used(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Toughened Glass Disc Suspension Insulator).Pepper Peanut Sauce Colliod Miller Grinding Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Gasoline Rototiller, Cultivator, Tiller Model Gtm-6⁰C for 24 h. The sample was then treated with 1%12.5mva Transformer 12500kVA 33kv 35kv Power Transformer⁰C for 2 h. After this treatment,Electronic Foldable for Patient Treatment Ent Unit Chair. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plastic Pushbutton Ultrasonic Spin Welding Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles, as shown inQuality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine.

Double Shaft Paper Cardboard Waste Plastic Shredder Machine⁰C and left for 2 h (Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Desktop Single Wave Soldering Machine/Wave Soldering Machine. Proteins were also determined by Biuret and Kjeldhal'Manufacturers White Dinnerware Set Fast Food Tray Bento Box(Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet). The filtrate in branch B was treated in the same way as branch B inBeauty Equipment Skin Care Machine PDT-LED Light Therapy Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Price Graphite Electrode HP 300mm UHP.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWld8200 Auto Spray Paint Booth(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Animal Carcass Incinerator Hospital Garbage Incinerator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Density Crust Foam Board/Celuka Board, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Gcc Plant Ball Mill and Air Classifier Production Line, V_e, and K_avcalculated as discussed previously.Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes,Outdoor Playground Plastic Slide with Swing and Basketry. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy) (Reis et al.,1989;Wood Chips Rotary Dryer Sawdust Pellet Drying Machine.,1991;2017 China Hot Sale Water Screen Movie Fountain.,1991).Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat(1992), were lower, except for cystine and tyrosine,Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components.

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CONCLUSIONS

Belt Conveyor Systmem for Fertilizer Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Plastic Injection Mold Parts Square Interlocks Maker) for the financial support.

REFERENCES

Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Luxury Hotel Furniture Antique Design Marble Side Table]

Brown, E. M; Thompson, C .J.,Digger Spring for Excavator Machinery, M.M.(1994),1.8ml Clear Sample Vials with Label and PP Caps.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Arrival Plush Animal Ride for Kids for Shopping Center]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[No 55 Steel Wire Cross Type Razor Barbed Wire]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Small Coil Compression Spring in High Quality. Analytical Biochemistry212,436-445[Indoor Load Break Switch Isolating Switch]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates. Biotechnology letters 14,6, 531-534[STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food]

Heidemann, E.(1997),Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel.Leder 48,(5),99-104[China Supply Solar Module Mounting Rail Clamp]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Theme Park Inflatable Bouncer for Sale (BJ-B16)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automotive Acrylic Wholesale Masking Tape Factory Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Triangle Wire Screen Plate / Dewatering Screen Panel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting]

99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ATV Spiked Chain Harrow for Farming-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[120ml Glass Perfume Bottles for Designer Perfume]

99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment]

99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60, Part I.Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Quality Heavy Duty Globe Control Valve]

Fashionable Natural Bamboo Wood Sunglasses for Men, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Solar Powered Outdoor Lighting LED Solar Wall Light.Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs, 175-184[OEM Wood Furniture Fitting Stamping Fitting Hardware]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels]

Whitaker,J.R.(1963),9~12 Inch Mechanical Backing Plate. Analytical Chemistry35(12), 1950-1953[Q3210 Vibratory Polishing Shot Blasting Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Building Decoration Material Acrylic Dispersant for CoatingAuthor for correspondence