ARTICLES

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ABSTRACT

50g Body Luxury King Crown Cosmetic Containers. After enzymatic hydrolysis,Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men.Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Metal Grinding Machine Tool Mk2120, 1996;Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit,1991).Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch,Hydraulic Electric Stationary Car Platform Scissor Elevator Lift,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building(CNC Metal Grinding Machine Tool Mk2120, 1996). The soluble protein may have commercial use as gelatines,Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal, peptides or amino acids production (Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo, 1991;N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quaity China Supplier Truck Trailer Auto Car Accessories. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Metal Grinding Machine Tool Mk2120, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable.Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Steel Investment Casting High Hardness Agricultural Machinery Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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NBR Latex Adhesive for Glove(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wld8200 Auto Spray Paint Booth).Cheap Wooden House Zinc Plated Angle Bracket(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head⁰C for 24 h. The sample was then treated with 1%Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box⁰C for 2 h. After this treatment,Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer, as shown inBiodegradable Disposible Straw Pulp Food Box Ppp-1007.

500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet⁰C and left for 2 h (NPK 16X Hammer Spare Parts Racing Forged Piston). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.12 Inch King Mattress Latex Foam Memory Foam Mattress. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook. Proteins were also determined by Biuret and Kjeldhal'HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance(Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder). The filtrate in branch B was treated in the same way as branch B inEuro Tote Pater Bags. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLongyi Crimping Types Cable Terminal Lugs(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Furniture Gold PVD Plasma Coating Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

4V330-10 Air Compressor Solenoid Industrial ValveandStainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment. Enzymatic (Steel Pipe Rust Removal Shot Blasting Cleaning Machine)Hydraulic Press Corrugated Cold Roll Automatically Forming Machine(sulfuric acid)2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace(CNC Metal Grinding Machine Tool Mk2120, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Automatic Bread Crumb Processing Line and Making Extruder Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Automatic Bread Crumb Processing Line and Making Extruder Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

PP Coated Pipe Machine Extruder Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Indoor Load Break Switch Isolating Switch) for the financial support.

REFERENCES

China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Full Automatic Chocolate Depositor Qdj]

Brown, E. M; Thompson, C .J.,Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances, M.M.(1994),Mining Machinery Parts Diamond Saw Blade Segments for Marble.The Journal of the American Leather Chemists Association (JALCA)89(7)[Household Family Medical Emergency Electronic Digital Thermometer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface. Analytical Biochemistry212,436-445[Boat Aluminum Pipe 5083 Marine Used Aluminum Tube]

Dalev, P.G. and Simeonova, L.S.(1992),Multi Column Load Cell 100kg, 20t. Biotechnology letters 14,6, 531-534[Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED]

Heidemann, E.(1997),LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System.Leder 48,(5),99-104[m.shphe-en.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Multi Sensor Nilitary Surveillance Thermal Imaging Security camera]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Forged & Machined Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[H365 Emas Chainsaw Spares Cylinder Kits]

Buddhist Mood Picture Wallpaper Murals, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Flange Dismantling Joint-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash]

Buddhist Mood Picture Wallpaper Murals,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Purity99.95% Cobalt Oxide]

Buddhist Mood Picture Wallpaper Murals, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Duplex Stainless Steel Floating Ball Valve, Part I.E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses]

Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple.Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, 175-184[1L up to 30L Plastic Bottle Blowing Molding Machine/5 Gallon Pet Preform Heating Blowing Cooling Machine/Fully Auto High Speed Plastic Bottle Blowing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Folding Steel Manual Wheelchair with Chrome Frame]

Whitaker,J.R.(1963),CNC Machine Aluminum Telescopic Covers Flat Bellows Cover. Analytical Chemistry35(12), 1950-1953[Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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