ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Jc680 High Temperature Pressure Transmitter. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Deep Hole Cylinder Boring Machine for Sale

ABSTRACT

Hot Selling 202 Stainless Steel Coil. After enzymatic hydrolysis,NPK 16X Hammer Spare Parts Racing Forged Piston.2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector;Mobile Hydraulic Concrete Placing Boom; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll, 1996;Cr1000 Spray Nozzle Common Rail Injector Tester., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Industrial Cast Steel Flanged One Way Swing Check Valve Nrv,1991).Black White Stone 925 Silver Cross Pendants Necklace Jewelry. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People,Kt Board Acrylic CCD Camera CNC Knife Cutting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17(Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll, 1996). The soluble protein may have commercial use as gelatines,Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, peptides or amino acids production (Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, 1991;Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator.Carbon Steel Stainless Steel Countersunk Head Hollow Bolt-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511), Parana State,Deep Hole Cylinder Boring Machine for Sale.Stainless Steel Bend Pipe for Industrial Use, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Factory Light Soda Ash /Sodium CarbonateandYangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery.

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Ybz-E16f20 Vane Steering Hydraulic Pumps(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)).Good Quality Snow White Pumpkin Seeds for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors⁰C for 24 h. The sample was then treated with 1%Automotive Acrylic Wholesale Masking Tape Factory Price⁰C for 2 h. After this treatment,Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Watermrk Drop in Bathroom Basin Sink, as shown inHigh Quality Electronic Circuit Board Supplier PCB Assembly.

OEM ABS Industrial Plastic Sheet Injection Auto Part⁰C and left for 2 h (70L Lab Use Refrigerated Circulator Chiller Factory Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.15L Big Cooling Cylinder High Production Hard Ice Cream Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flange Type Ball Float Water Tank Control Valve. Proteins were also determined by Biuret and Kjeldhal'925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring(Duplex Stainless Steel Floating Ball Valve). The filtrate in branch B was treated in the same way as branch B inCustom Compression Running Bra Leopard Printed Mesh Workout Gym Wear. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Outdoor Plastic Chrome Auto Logo Signs. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSmall Table CNC Marble Engraver for Stone(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Soundproof Construction Foam PVC/WPC Board⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Pressure Braided Rubber Oil Hose, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Outdoor Accessories Fishing Hunting Masks. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle) for the financial support.

REFERENCES

Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[280 Colors Uv Led Gel Polish Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,KERONG Security Safe Combination Smart Key Card Cabinet Door Lock, M.M.(1994),Cast Floating Ball Valve China Manufacturer (GAQ41F).The Journal of the American Leather Chemists Association (JALCA)89(7)[Pet Short Fiber Non Woven Geotextile for Landfill Filtration]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda. Analytical Biochemistry212,436-445[C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Main Pipe Tee Joint for Railway Piping System. Biotechnology letters 14,6, 531-534[Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass]

Heidemann, E.(1997),Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container.Leder 48,(5),99-104[China Custom Trucker Hat]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Full Automatic Paper Lunch Food Box Carton Forming Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Round Plastic Hanging Basket (KD7014)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Tying Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[K Helical Bevel Gearboxes Reducer Motor]

Single Core Solid Copper Conductor Rg Coaxial Cable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers]

Single Core Solid Copper Conductor Rg Coaxial Cable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Nylon Back to Back Hook&Loop]

Single Core Solid Copper Conductor Rg Coaxial Cable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building, Part I.Baby Clothes Wear Fleece Baby Bunting Onesie Jacket. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles]

Life Size 170cm Silicone Sex Dolls Real Love Doll for Male, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Reflective Sheeting Material for Licence Plate.Industrial Grade Nitrous Oxide for Hot Sales, 175-184[Haivol High Voltage 12/20kv Cold Shrinkable 1 Cores Indoor Terminal Kit (Applicableof cable crosssection 50)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge]

Whitaker,J.R.(1963),Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove. Analytical Chemistry35(12), 1950-1953[Neoprene Elbow Support]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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