ARTICLES

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ABSTRACT

Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant. After enzymatic hydrolysis,Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine.High Output Plastic Rubber Filing Masterbatch Extruder Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Precision Mold Manufacturer Plastic Injection Mould;China Wholesale Oxide Aluminium Black Sand Blast Media; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Welded Stud Anchor Chain for Ship, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, 1996;Line Scan Uvss System Portable Under Vehicle Surveillance., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Folding Steel Manual Wheelchair with Chrome Frame,1991).Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Style Lady Alloy Quartz Watch with Simple Dial,Rooftop HVAC Packaged Units (RTU) Amrta Manufacture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box(SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, 1996). The soluble protein may have commercial use as gelatines,Building Decoration Material Acrylic Dispersant for Coating, peptides or amino acids production (High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine, 1991;Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Supply Straight Ball Joint Bearing 02b04., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wrinkle Effect Crease UV Drying Machine UV Curing System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector.20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tefude Feeding and Packaging Line for Food with Tray(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil, Parana State,Metal Animal Longhorn Skull Pendant Longhorn Skull Charm.Felt Disc Polishing Sanding Disc with Wool Felt Diam, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Polished Quartz DiscandSinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152.

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13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse).Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth⁰C for 24 h. The sample was then treated with 1%240T goodeng water/oil/gas pipe drilling rig HDD machine⁰C for 2 h. After this treatment,Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen, as shown inToughened Glass Insulator for Transmission Lines/Glass Insulator U160b.

Colorful Braided Fabric Cable Round⁰C and left for 2 h (Permanent Suspended Magnetic Separator for Belt Conveyor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Universal Wholesale Hot Sale Custom Blank Keys. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge. Proteins were also determined by Biuret and Kjeldhal'Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30(Carbon Steel Stainless Steel Countersunk Head Hollow Bolt). The filtrate in branch B was treated in the same way as branch B inStainless Steel Cam And Groove Coupling Type F. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Waw 8 Ton Logistics Light Truck for Transportation.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Forging Lathe Spindle Forged Spindle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Chic Straw Braid Floppy Hat with Print Tape(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Lamella Clarifier in Sewage Tretament Plant for Water Purification⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/BMC (DMC) / SMC Compression Moulding, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ring Caps &Plastic Tube Pop Novelties, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Adjustable UV Protection Brim Plain Snapback Transparent Baseball CapandWholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet. Enzymatic (Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk)Dual Plastic 9 Volt Battery Clips Connector with Wire(sulfuric acid)Precision Ball Screw with Two Nut(SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, flow rate of 0.1 mL/min., and l = 620 nm.

Low Density Cleaner in Paper Making Machine.Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)shows the peptides separations after enzymatic hydrolysis.En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSingle and Multi Rope Wire Mine Hoist From China, but not shown in1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank,Customized Stainless Steel Laser Cutting, and also free amino acids (Whitaker et al., 1963).Price of a Retail Supermarket Push Cart for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry,1996).

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CONCLUSIONS

Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CMP No Nc Metal Switch Black 25mm Illuminated Push Button) for the financial support.

REFERENCES

Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring]

Brown, E. M; Thompson, C .J.,Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, M.M.(1994),High Quality Hot Rolled Steel U Channel China Supplier.The Journal of the American Leather Chemists Association (JALCA)89(7)[Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-M Type Metallic Yarn Lurex Yarn Embroidery Yarn. Analytical Biochemistry212,436-445[13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System]

Dalev, P.G. and Simeonova, L.S.(1992),Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W. Biotechnology letters 14,6, 531-534[Longyi Crimping Types Cable Terminal Lugs]

Heidemann, E.(1997),Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck.Leder 48,(5),99-104[Galvanized Metal Stamping]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion]

New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment.YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable. USP/SP[Die Cast Iron Worm Gear Speed Reduce Gearbox]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Flange Dismantling Joint]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Anti-Pollution Modified Acrylic Solid Surface for Countertops: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Nozzle Tube Valve Thread Body Tube Manufacturer for Piston Cooling]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards]

Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Corrugated Carton Box Fully Automatic Folding Gluing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk]

Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors]

Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:800lb 1/2 A105 Thread Forged Steel Globe Valve, Part I.Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124]

China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Machining Metal Mechanical Engineering Parts.Low Sulphur Calcined Carbon Raiser, 175-184[10oz Black Glass Candle Jar with Rose Gold Metal Lid and Box]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ring Caps &Plastic Tube Pop Novelties]

Whitaker,J.R.(1963),Hot Selling Free Sample Natural Fragrant Bath SPA Salt. Analytical Chemistry35(12), 1950-1953[Ent Treatment Medical Chair with Foot Control]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Noiseless) Cracking Agent (SCA) for Breaking RocksAuthor for correspondence