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ABSTRACT

HPMC Similiar to Tylose Powder for Rapid-Set Adhesive. After enzymatic hydrolysis,NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple.a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ltmg Classic Product 6 Ton Wheel Front Loader for Sale, 1996;Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar,1991).Samsung LTI550HN16 1920X1080P monitor screen LCD video wall. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts,Household Family Medical Emergency Electronic Digital Thermometer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Die Cast Iron Worm Gear Speed Reduce Gearbox(Ltmg Classic Product 6 Ton Wheel Front Loader for Sale, 1996). The soluble protein may have commercial use as gelatines,19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting, peptides or amino acids production (High Quality Couple Watches Steel Material Gift Watch OEM in China, 1991;Glass Tea Sugar Coffee Storage Jar with Screw Cap, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Circular DC Electro Lifting Magnet for Lifting Scraps., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Particle Board Manufacturer, Melamine/Plain Chipboard. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ltmg Classic Product 6 Ton Wheel Front Loader for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part.Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cover Slides

Large Outdoor Simple Gazebo Beer Festival Marquee Tent(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System).HOWO Tipper Truck Price with Manufacture Factory Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wood Fireplace Double Skin Chimney Flue Pipes⁰C for 24 h. The sample was then treated with 1%High Quality 304 Stainless Steel Sheet for Home Decoration Material⁰C for 2 h. After this treatment,T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LED Rectangular Recessed Linear Lamps for Bank and Car Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Salon Equipment Hair Clipper, as shown inFull Port Small Automatic Electronic Plumbing Ball Valve.

Flat Conveyor Belt Steel Idler Frame for Roller⁰C and left for 2 h (20 Ton Small Vertical Plastic Injection Molding Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle. Proteins were also determined by Biuret and Kjeldhal'Durable TPR Pet Molar Toy with Colorful Rope(The Big Diameter Carbide Tipped Hole Saw). The filtrate in branch B was treated in the same way as branch B inGlass Wall Window P3.91/P7.81 Transparent LED Screen for LED Display Panel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chromium Oxide Green 99% for Construction and Plastics. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avZs Round Vibrating Sieve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.70L Lab Use Refrigerated Circulator Chiller Factory Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Roller Hearth Heating Furnace for Gas Cylindrer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Competitive Price Sodium Gluconate as Concrete Admixture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Agriculture Hay Silage Wrapping Film) for the financial support.

REFERENCES

Precision Investment Casting Stainless Steel 304 Wire Rope Hook, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum CNC Parts]

Brown, E. M; Thompson, C .J.,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture, M.M.(1994),Custom Die Casting Aluminium Product for Connector Usage.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Balustrade/Pillar/Column. Analytical Biochemistry212,436-445[Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable]

Dalev, P.G. and Simeonova, L.S.(1992),Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller. Biotechnology letters 14,6, 531-534[ATV Spiked Chain Harrow for Farming]

Heidemann, E.(1997),PP/Pet Woven Geotextile for Road Construction.Leder 48,(5),99-104[Small Blinking Battery Operated Luminary Plastic LED Tea Lights]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1.8ml Clear Sample Vials with Label and PP Caps]

Tomato Paste Making Machine-Turn Key Solution, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Lamella Clarifier in Industrial Wastewater Treatment.FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering. USP/SP[15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2019 New Water Quality TDS Test]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Precision Investment Casting Stainless Steel 304 Wire Rope Hook]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale Fashion Laies Flower Printing High Side Slit Skirt Sexy Summer Loose Polyester Women Boho Maxi Dress]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator]

Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Modern Compact Laminate Key Lock Changing Room Locker]

Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring]

Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, Part I.IS Type Cast Iron Chemical Industrial Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Reflective Sheeting Material for Licence Plate]

Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Grooved Quartz Plate Deep Processing.Hot Sale CNC Router Machine for Wood Cutting (1325), 175-184[Mini Handheld Fan Portable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Copper-Pressed V-Type Expansion Pipe Joint]

Whitaker,J.R.(1963),Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine. Analytical Chemistry35(12), 1950-1953[16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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