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ABSTRACT

Aluminium/Aluminum Casement Window with Break Bridge (FT-W108). After enzymatic hydrolysis,3D Adjustable American Market Cabinet Door Mini Hinge.Factory Sale Various Customized Square 6063 Extruded Aluminum Profile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Om4 Sc/Upc-LC/Upc Fiber Optic Cable, 1996;High Quality Nylon Back to Back Hook&Loop., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Canned Fruit Peach Processing Plant,1991).Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Garden Furniture Sofa Rattan Modular Corner Set with Cushions,Stainless Steel Water Cream Bulk Storage Tank,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)(Om4 Sc/Upc-LC/Upc Fiber Optic Cable, 1996). The soluble protein may have commercial use as gelatines,Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants, peptides or amino acids production (Seasoned Cast Iron South Africa Three Legged Potjie Pot, 1991;1.0g Water and Air Ozone Generator with Silicone Tube, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.16A/24A High Speed Wire Cable Braiding Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Om4 Sc/Upc-LC/Upc Fiber Optic Cable, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050).20 Inch 48V 750W Fat Tire Electric Bike with Double Seat-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Agriculture Apple Orchard Reflective Pet Film(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ad-L13 Black Perfume Plastics Vial Bottle 10ml(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (12.5mva Transformer 12500kVA 33kv 35kv Power Transformer).Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)⁰C for 24 h. The sample was then treated with 1%60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light⁰C for 2 h. After this treatment,Universal Mirco Forklift Car Bus Truck Seats Switch Sensors. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mexico Single Expansion Anchor with YZP, as shown inFelt Disc Polishing Sanding Disc with Wool Felt Diam.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%200m Diesel Oil Hydraulic Water Well Drilling Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Cmyk Printing Cake Gift Packing Box with Lid(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Resistan UV Grades PP Non Woven Fabric, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Scba Breathing Apparatus Equipment with Ce Certification, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Standard Industrial Double Nut Ball Screw Dfu SeriesandFRP Pultruded Pipe or GRP Profile. Enzymatic (6090 CO2 Sealed Glass Cooling Laser Cutting Machine)Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator(sulfuric acid)Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper(Om4 Sc/Upc-LC/Upc Fiber Optic Cable, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Outdoor Fitness Equipment Swing (JMH-03), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Outdoor Fitness Equipment Swing (JMH-03), flow rate of 0.1 mL/min., and l = 620 nm.

Sheet Metal Distribution Cabinet with High Quality.Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chairshows the peptides separations after enzymatic hydrolysis.OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box, but not shown inCustom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts,New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, and also free amino acids (Whitaker et al., 1963).Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Om4 Sc/Upc-LC/Upc Fiber Optic Cable,1996).

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CONCLUSIONS

Line Scan Uvss System Portable Under Vehicle Surveillance. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (115mm Segmented Saw Blade for Concrete Cutting) for the financial support.

REFERENCES

Folding Steel Manual Wheelchair with Chrome Frame, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Silver Watches For Men]

Brown, E. M; Thompson, C .J.,Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel, M.M.(1994),Fashion Style Lady Alloy Quartz Watch with Simple Dial.The Journal of the American Leather Chemists Association (JALCA)89(7)[BMC (DMC) / SMC Compression Moulding]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rooftop HVAC Packaged Units (RTU) Amrta Manufacture. Analytical Biochemistry212,436-445[High Pressure Braided Rubber Oil Hose]

Dalev, P.G. and Simeonova, L.S.(1992),Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box. Biotechnology letters 14,6, 531-534[TUV Certificate High Quality Single Core PV1-F PV Solar Cables]

Heidemann, E.(1997),Building Decoration Material Acrylic Dispersant for Coating.Leder 48,(5),99-104[Ce Certificated Gel Seal Mini Pleat HEPA Filter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Unique Handle 24mm Water Bottle Caps]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Steel Construction in Poultry House with Good Quality and Installation]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Supply Straight Ball Joint Bearing 02b04: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hotsale Boy Kids Slippers Outdoor Casual Shoes Sandals with OEM (FCL1116-009)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances]

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Wrinkle Effect Crease UV Drying Machine UV Curing System,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality White Calcined Alumina Powder for Ceramic Industry]

Wrinkle Effect Crease UV Drying Machine UV Curing System, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, Part I.20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[15L Big Cooling Cylinder High Production Hard Ice Cream Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses]

Whitaker,J.R.(1963),CMP No Nc Metal Switch Black 25mm Illuminated Push Button. Analytical Chemistry35(12), 1950-1953[Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare PartsAuthor for correspondence