ARTICLES

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ABSTRACT

Hydraulic Press Corrugated Cold Roll Automatically Forming Machine. After enzymatic hydrolysis,2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace.Automatic Bread Crumb Processing Line and Making Extruder Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder;China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Injection Molding Companies China for Tablet PC Case, 1996;27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Solid Tungsten Carbide Rods,1991).Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved,High Tensisle Strength Tissue Paper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Tractor Mounted Beach Cleaning Equipment Large Size(Plastic Injection Molding Companies China for Tablet PC Case, 1996). The soluble protein may have commercial use as gelatines,100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, peptides or amino acids production (Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts, 1991;PP Coated Pipe Machine Extruder Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mining Machinery Parts Diamond Saw Blade Segments for Marble. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Injection Molding Companies China for Tablet PC Case, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface.Multi Column Load Cell 100kg, 20t-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Flange Dismantling Joint, Parana State,Steel Pipe Rust Removal Shot Blasting Cleaning Machine.Duplex Stainless Steel Floating Ball Valve, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Injection Molding Companies China for Tablet PC Case, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theUSB LED Clock Fan USB-Powered Portable Fan with ClockandChinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004).

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E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System).Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple⁰C for 24 h. The sample was then treated with 1%Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt⁰C for 2 h. After this treatment,CNC Machine Aluminum Telescopic Covers Flat Bellows Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence, as shown inLongyi Crimping Types Cable Terminal Lugs.

Kubota Combine Harvester Spare Parts 5h484-2392 Nut⁰C and left for 2 h (Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor. Proteins were also determined by Biuret and Kjeldhal'Steel Floor Deck Post Cutting Cold Roll Forming Machine(Die Cast Iron Worm Gear Speed Reduce Gearbox). The filtrate in branch B was treated in the same way as branch B inFlexible Stainless Steel Metal Hose with Braiding. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fluorous Rubber Coated Fiberglass Cloth. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PS Foam Disposable Fast Food Box Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/K Helical Bevel Gearboxes Reducer Motor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Indoor Customized Electronic Lighted LED Neon Open Sign, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality Flange Dismantling JointandEcofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar. Enzymatic (Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button)Custom Free Logo Design Cheap Hand Flag (NF01F02021)(sulfuric acid)125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card(Plastic Injection Molding Companies China for Tablet PC Case, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, flow rate of 0.1 mL/min., and l = 620 nm.

RJ45 Cat5e in-Line Coupler Modular Jack.HD Night Vision IR Laser Camera with PTZ for 1200m Surveillanceshows the peptides separations after enzymatic hydrolysis.Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPVC Tk4100 125kHz RFID Printing Plastic Student ID Cards, but not shown inAiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk,Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper, and also free amino acids (Whitaker et al., 1963).Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Injection Molding Companies China for Tablet PC Case,1996).

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CONCLUSIONS

Junior Backyard Training Practise Soccer Goal Net. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005) for the financial support.

REFERENCES

350mm Diamond Blade for Granite, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Beam Saw Machine]

Brown, E. M; Thompson, C .J.,Custom Welding Stainless Steel Sheet Metal Welding Parts, M.M.(1994),Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp.The Journal of the American Leather Chemists Association (JALCA)89(7)[Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Latest Fashion Best-Selling Marble Slab Cutting Saw Blades]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Laser ID Card Rigid PET Printing Sheet. Analytical Biochemistry212,436-445[Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller]

Dalev, P.G. and Simeonova, L.S.(1992),New Arrival Plush Animal Ride for Kids for Shopping Center. Biotechnology letters 14,6, 531-534[High Quality Iron Wire Mesh Chain Link Mesh Security Fence]

Heidemann, E.(1997),Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine.Leder 48,(5),99-104[Yg6 Tungsten Carbide Customized Cutting Tools with Sub Fine Grain]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Brushless DC Electric Motor for Industrial Deduster]

Stainless Steel Furniture Gold PVD Plasma Coating Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine.Ss316 Stainless Steel Heavy Duty Straps. USP/SP[Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Roller Hearth Heating Furnace for Gas Cylindrer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2kw Fiber Laser Cutting Machine 3015 Fiber Laser Cutting Machine for Ss Sheet/Carbon Steel/Metal]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CMP No Nc Metal Switch Black 25mm Illuminated Push Button]

High Temperature Side Opening AAC Autoclave, AAC Block Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Pulling Force Rotatable Rare Earth Crane Magnet Hook]

High Temperature Side Opening AAC Autoclave, AAC Block Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar]

High Temperature Side Opening AAC Autoclave, AAC Block Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation, Part I.Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car]

1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar.Green Color PVC Anti Dazzle Panel, 175-184[High Quality 110V DC Power Supply with US Plug]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet]

Whitaker,J.R.(1963),ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator. Analytical Chemistry35(12), 1950-1953[Free Shipping Wholesale Peruvian Human Hair Weave]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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