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ABSTRACT

High Purity99.95% Cobalt Oxide. After enzymatic hydrolysis,Wood Activated Carbon for Decolorizing in Food Industry with ASTM.Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated, 1996;6000 Series Anodizing Extrusion Aluminum Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J),1991).DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tomato Paste Making Machine-Turn Key Solution,Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine(Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated, 1996). The soluble protein may have commercial use as gelatines,HPMC Similiar to Tylose Powder for Rapid-Set Adhesive, peptides or amino acids production (NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple, 1991;a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Selling Solidwood Viola Wholesale Price Viola., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Building Material Sound Heat Insulation Rock Wool Sandwich Panel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ltmg Classic Product 6 Ton Wheel Front Loader for Sale.Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Glass Tea Sugar Coffee Storage Jar with Screw Cap(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Leaf Shape Pearl Jewelry Wedding Tiara).Circular DC Electro Lifting Magnet for Lifting Scraps(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts⁰C for 24 h. The sample was then treated with 1%Particle Board Manufacturer, Melamine/Plain Chipboard⁰C for 2 h. After this treatment,CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, as shown inPaintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%16oz Insulated Cardboad Cup Sleeve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLarge Outdoor Simple Gazebo Beer Festival Marquee Tent(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wood Fireplace Double Skin Chimney Flue Pipes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality 304 Stainless Steel Sheet for Home Decoration Material, pH 3.25., mobile phase B: buffer solution of 0.01 mol/T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Stainless Steel DIN7981 Pan Head Self Tapping Screw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Household Family Medical Emergency Electronic Digital Thermometer) for the financial support.

REFERENCES

19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electrostatic Powder Coating Machine/ Electrostatic Spray Painting Equipment]

Brown, E. M; Thompson, C .J.,Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax, M.M.(1994),Dark Green Decorative Garden Welded Wire Mesh Fencing.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Speed Steel Mill Roll with High Quality]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Csm DIY Microscope for Kids/Toy Microscope. Analytical Biochemistry212,436-445[CNC Machine Aluminum Telescopic Covers Flat Bellows Cover]

Dalev, P.G. and Simeonova, L.S.(1992),Oak Veneer Laminated Plywood for Decoration. Biotechnology letters 14,6, 531-534[Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Price 4X6 Acrylic Magnetic Photo Frame]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wld8200 Auto Spray Paint Booth]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Jumping Trampolines]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Biodegradable Disposible Straw Pulp Food Box Ppp-1007]

High Quality Women′s Suit in Reasonable Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pet Short Fiber Non Woven Geotextile for Landfill Filtration-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[NPK 16X Hammer Spare Parts Racing Forged Piston]

High Quality Women′s Suit in Reasonable Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder]

High Quality Women′s Suit in Reasonable Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof, Part I.Competitive Price Sodium Gluconate as Concrete Admixture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4V330-10 Air Compressor Solenoid Industrial Valve]

Precision Investment Casting Stainless Steel 304 Wire Rope Hook, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture.Custom Die Casting Aluminium Product for Connector Usage, 175-184[Car Front Head Lamp]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1 Ton Toyo Type Manual Chain Block]

Whitaker,J.R.(1963),Stainless Steel Balustrade/Pillar/Column. Analytical Chemistry35(12), 1950-1953[High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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