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ABSTRACT

Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry. After enzymatic hydrolysis,Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold.Industrial P-Toluene Sulfonic Acid for 93%-99% Purity. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine;Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Purity99.95% Cobalt Oxide, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wood Activated Carbon for Decolorizing in Food Industry with ASTM, 1996;Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine,1991).8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net,Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,6000 Series Anodizing Extrusion Aluminum Tube(Wood Activated Carbon for Decolorizing in Food Industry with ASTM, 1996). The soluble protein may have commercial use as gelatines,World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J), peptides or amino acids production (DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W, 1991;Tomato Paste Making Machine-Turn Key Solution, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-HPMC Similiar to Tylose Powder for Rapid-Set Adhesive. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wood Activated Carbon for Decolorizing in Food Industry with ASTM, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple.a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Selling Solidwood Viola Wholesale Price Viola(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Building Material Sound Heat Insulation Rock Wool Sandwich PanelAlcalase2.5 L DX,Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Ltmg Classic Product 6 Ton Wheel Front Loader for Sale); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting).Household Family Medical Emergency Electronic Digital Thermometer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Die Cast Iron Worm Gear Speed Reduce Gearbox⁰C for 24 h. The sample was then treated with 1%19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting⁰C for 2 h. After this treatment,High Quality Couple Watches Steel Material Gift Watch OEM in China. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Glass Tea Sugar Coffee Storage Jar with Screw Cap's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Circular DC Electro Lifting Magnet for Lifting Scraps, as shown inCustomized Outdoor Plastic Chrome Auto Logo Signs.

Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts⁰C and left for 2 h (Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Particle Board Manufacturer, Melamine/Plain Chipboard. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part. Proteins were also determined by Biuret and Kjeldhal'Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)(Q235 Q345 Hot Rolled Channel Steel). The filtrate in branch B was treated in the same way as branch B inAir Cooler Evaporator Condenser Series Fn-4.4. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av120ml Glass Perfume Bottles for Designer Perfume(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane, pH 3.25., mobile phase B: buffer solution of 0.01 mol/16oz Insulated Cardboad Cup Sleeve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2andLarge Outdoor Simple Gazebo Beer Festival Marquee Tent. Enzymatic (HOWO Tipper Truck Price with Manufacture Factory Price)Wood Fireplace Double Skin Chimney Flue Pipes(sulfuric acid)High Quality 304 Stainless Steel Sheet for Home Decoration Material(Wood Activated Carbon for Decolorizing in Food Industry with ASTM, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant, flow rate of 0.1 mL/min., and l = 620 nm.

LED Rectangular Recessed Linear Lamps for Bank and Car Sale.Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabricshows the peptides separations after enzymatic hydrolysis.Salon Equipment Hair Clipper(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25), but not shown inBeauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine,Flat Conveyor Belt Steel Idler Frame for Roller, and also free amino acids (Whitaker et al., 1963).LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wood Activated Carbon for Decolorizing in Food Industry with ASTM,1996).

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Chromium Oxide Green 99% for Construction and Plastics, V_e, and K_avcalculated as discussed previously.Zs Round Vibrating Sieve,Stainless Steel Tactile Paving Blind Road Stud. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003) (Reis et al.,1989;DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W.,1991;Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car.,1991).70L Lab Use Refrigerated Circulator Chiller Factory Price(1992), were lower, except for cystine and tyrosine,Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500).

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CONCLUSIONS

Roller Hearth Heating Furnace for Gas Cylindrer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ltmg Classic Product 6 Ton Wheel Front Loader for Sale) for the financial support.

REFERENCES

custom design MIFARE DESFire EV2 2K plastic RFID loyalty card, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[RF Shielding MRI Door with Brass Frame for RF Cage]

Brown, E. M; Thompson, C .J.,Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016, M.M.(1994),Hotel Card Door Lock Access Control Safe Door Lock.The Journal of the American Leather Chemists Association (JALCA)89(7)[12.5mva Transformer 12500kVA 33kv 35kv Power Transformer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Felt Disc Polishing Sanding Disc with Wool Felt Diam]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer. Analytical Biochemistry212,436-445[Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel DIN7981 Pan Head Self Tapping Screw. Biotechnology letters 14,6, 531-534[Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert]

Heidemann, E.(1997),19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS.Leder 48,(5),99-104[0.98t5m Marine Ship Deck Crane Hydraulic Knuckle and Telescopic Boom]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Standard Industrial Double Nut Ball Screw Dfu Series]

Newest Men Watches Popular Products Mens Custom Wood Watch, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax.Dark Green Decorative Garden Welded Wire Mesh Fencing. USP/SP[Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Csm DIY Microscope for Kids/Toy Microscope: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Rope Clip]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor Full Color LED Video Wall]

Oak Veneer Laminated Plywood for Decoration, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Selling Free Sample Natural Fragrant Bath SPA Salt]

Oak Veneer Laminated Plywood for Decoration,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose]

Oak Veneer Laminated Plywood for Decoration, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl, Part I.High Speed HDMI Cable Wholesale HDMI with Ethernet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw]

Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Women′s Suit in Reasonable Price.Pet Short Fiber Non Woven Geotextile for Landfill Filtration, 175-184[Stainless Steel Spring PTFE Bronze Energized Seals]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button]

Whitaker,J.R.(1963),Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof. Analytical Chemistry35(12), 1950-1953[ Rapid]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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