ARTICLES

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ABSTRACT

Flanged Braided Metal Hose Connector. After enzymatic hydrolysis,Timber Pressure Vessel for Wood Processing.1200mm Recessed Mounted LED Linear Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, 1996;Fashion Men′s Large Dial Casual Quartz Watch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence,1991).Outdoor Garden Furniture Baby Plastic Swing for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone,2D LED Rope Lighting Motif Lights for Christmas Decoration,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modern Compact Laminate Key Lock Changing Room Locker(Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, 1996). The soluble protein may have commercial use as gelatines,Outdoor Full Color LED Video Wall, peptides or amino acids production (180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125), 1991;Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Quick Connect Camlock and Grooved Couplings Quick Fitting Type D.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality White Calcined Alumina Powder for Ceramic Industry. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine.100kw Cummins Diesel Generator Set-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Professional Manufacture Metal Medal Promotional Gift).5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant⁰C for 24 h. The sample was then treated with 1%SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel⁰C for 2 h. After this treatment,High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100 Liter Cooking Pots for Jam's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Food Grade Customized Metal Candy Can Square Mint Tin Box, as shown inSurveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera.

600X600 mm Ugr 19 LED Panel Lights⁰C and left for 2 h (Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Good Quality Hot Tube8 Japanese Stainless Steel Tube 666. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine. Proteins were also determined by Biuret and Kjeldhal'Round Plastic Hanging Basket (KD7014)(3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine). The filtrate in branch B was treated in the same way as branch B inALS Stage 5W RGB Animation Laser Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Steel Setractable Rail Protection Cover⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Natural Birch F&B Veneer Furniture Plywood, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Spark Resistant Construction Axial Fan with Fixed Pitch Blade. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Commercial Double Lane Inflatable Carnival Bungee Run Game) for the financial support.

REFERENCES

Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Best Quality Raw Melamine Decorative Paper for MDF Laminating]

Brown, E. M; Thompson, C .J.,3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home, M.M.(1994),25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25).The Journal of the American Leather Chemists Association (JALCA)89(7)[240T goodeng water/oil/gas pipe drilling rig HDD machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Precision CNC Milling DIN69871. Mas403 for CNC Machinist. Analytical Biochemistry212,436-445[Wholesale Flower Embroidery Applique Patches]

Dalev, P.G. and Simeonova, L.S.(1992),Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect. Biotechnology letters 14,6, 531-534[Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting]

Heidemann, E.(1997),Building Material of Granite Ceramic Wall Tile for Wall Decoration.Leder 48,(5),99-104[Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Outdoor Plastic Chrome Auto Logo Signs]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Q235 Q345 Hot Rolled Channel Steel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[standard size CR80 T5577 PVC access control RFID blank card]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric]

Precision Customized CNC Machined Mild Steel Flange for Automobile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)]

Precision Customized CNC Machined Mild Steel Flange for Automobile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine]

Precision Customized CNC Machined Mild Steel Flange for Automobile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Metal Container Packaging for Edible Oil Tin Cans Storage Boxes, Part I.Melee Custom Plastic Mould New Design. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Reusable PLA and Pbat Tea Packing Pouch Supplier in China]

High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Copper-Pressed V-Type Expansion Pipe Joint.Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber, 175-184[Carbon Fiber Irreguar Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Big Smart Hydroponic Planter Indoor Garden Light]

Whitaker,J.R.(1963),White Desiccant Bag Silica Gel with Indicated Orange Bead. Analytical Chemistry35(12), 1950-1953[OEM Viton Mechanical Rubber Oil Seal]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/HardwareAuthor for correspondence