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ABSTRACT

Professional Manufacturer Stainless Steel Floor Grating. After enzymatic hydrolysis,Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels.Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324, 1996;Ce Quality Double Channel Medical Syringe Pump (THR-SP180D)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity,1991).High Quality Rubber Pressed Mixing Machine with Ce Certification. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600,4K Giant Whiteboard Interactive Panel for University Whiteboard,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid(DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324, 1996). The soluble protein may have commercial use as gelatines,Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310), peptides or amino acids production (Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting, 1991;Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Practical Edible Silicone Kitchenware (YB-ty-29)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.4 Inch Quarter Round PCD Diamond Grinding Cup Wheel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG).Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, Parana State,Round Hole Carbon Steel Pipe Perforated for Filter.Custom Design 100% Silk Tie for Men, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the WireandPrecision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts.

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Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector).Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Om4 Sc/Upc-LC/Upc Fiber Optic Cable⁰C for 24 h. The sample was then treated with 1%High Quality Nylon Back to Back Hook&Loop⁰C for 2 h. After this treatment,Canned Fruit Peach Processing Plant. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Garden Furniture Sofa Rattan Modular Corner Set with Cushions, as shown inQC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery.

Stainless Steel Water Cream Bulk Storage Tank⁰C and left for 2 h (China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants. Proteins were also determined by Biuret and Kjeldhal'Seasoned Cast Iron South Africa Three Legged Potjie Pot(YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable). The filtrate in branch B was treated in the same way as branch B inAmbulatory Monitor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1.0g Water and Air Ozone Generator with Silicone Tube.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av16A/24A High Speed Wire Cable Braiding Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/20 Inch 48V 750W Fat Tire Electric Bike with Double Seat, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Agriculture Apple Orchard Reflective Pet Film, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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3D Creative Design Metal Medal Promotion Gift of Sport.Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substituteshows the peptides separations after enzymatic hydrolysis.Ad-L13 Black Perfume Plastics Vial Bottle 10ml(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Supply Coaxial Cable Rg 59 with Messenger, but not shown inWholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle,Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, and also free amino acids (Whitaker et al., 1963).EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324,1996).

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Mexico Single Expansion Anchor with YZP, V_e, and K_avcalculated as discussed previously.Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe,SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (NBR Latex Adhesive for Glove) (Reis et al.,1989;Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting.,1991;Ce Quality Double Channel Medical Syringe Pump (THR-SP180D).,1991).Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope(1992), were lower, except for cystine and tyrosine,Whole Absorbtion Flexo Printing Soltting Die Cutting Machine.

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CONCLUSIONS

Factory Price 4X6 Acrylic Magnetic Photo Frame. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminium/Aluminum Casement Window with Break Bridge (FT-W108)) for the financial support.

REFERENCES

200m Diesel Oil Hydraulic Water Well Drilling Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sale Deep Embossed Vinyl 1.06m Wallpaper for Wall Coating]

Brown, E. M; Thompson, C .J.,Custom Cmyk Printing Cake Gift Packing Box with Lid, M.M.(1994),Air Purification Activated Alumina.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Electric Piston Pump Coating Machine PT6900]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6). Analytical Biochemistry212,436-445[Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace]

Dalev, P.G. and Simeonova, L.S.(1992),Resistan UV Grades PP Non Woven Fabric. Biotechnology letters 14,6, 531-534[1200mm Recessed Mounted LED Linear Light]

Heidemann, E.(1997),New Scba Breathing Apparatus Equipment with Ce Certification.Leder 48,(5),99-104[Rg-58/U RF Coaxial Cable 20 AWG Shielded Wire Cord for Satellite TV Box]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use]

Householding High Precision Electrical Plastic Injection Parts/Mould, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),FRP Pultruded Pipe or GRP Profile.6090 CO2 Sealed Glass Cooling Laser Cutting Machine. USP/SP[High Quality Crusher for Used Plastic Recycling Line]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Outdoor Accessories Fishing Hunting Masks]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Shell Activated Carbon Series]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Low Sulphur Calcined Carbon Raiser]

Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor Fitness Equipment Swing (JMH-03)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[12V 24V 220V Digital Temperature Controller]

Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine]

Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sheet Metal Distribution Cabinet with High Quality, Part I.OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cattle Corpse Cremation Burning Stove]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Natural Birch F&B Veneer Furniture Plywood]

Whitaker,J.R.(1963),Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant. Analytical Chemistry35(12), 1950-1953[800mm Length 600n Load Tension Gas Spring with Metal Eyelet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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