ARTICLES

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ABSTRACT

High Speed Steel Mill Roll with High Quality. After enzymatic hydrolysis,3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer.2017 China Hot Sale Water Screen Movie Fountain. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse , 1996;Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Latest New Hho Engine Oil Carbon Remover,1991).Wood Chips Rotary Dryer Sawdust Pellet Drying Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Delicate Handmade Round Natural Stone Chain Necklace,Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light(Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse , 1996). The soluble protein may have commercial use as gelatines,Embossed Aluminium/ Aluminum Household Foil 8011-O, peptides or amino acids production (High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021), 1991;Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cross Hex Flange Screw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Full Color Indoor Outdoor LED Curtain TV Display. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse , 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Plastic Injection Mold Parts Square Interlocks Maker.LED Outdoor Solar Lawn Garden Lighting for Wall-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3-Pin Australia Power Cord Plug with SAA(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Sludge Oil Separation CentrifugeAlcalase2.5 L DX,8011 Aluminum Foil for Cap Used, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Pepper Peanut Sauce Colliod Miller Grinding Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Gasoline Rototiller, Cultivator, Tiller Model Gtm-6(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

12.5mva Transformer 12500kVA 33kv 35kv Power Transformer, Parana State,15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine.Electronic Foldable for Patient Treatment Ent Unit Chair, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse , 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGear Rack and Pinion for Construction HoistandHigh Density Crust Foam Board/Celuka Board.

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Plastic Pushbutton Ultrasonic Spin Welding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine).500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Double Shaft Paper Cardboard Waste Plastic Shredder Machine⁰C for 24 h. The sample was then treated with 1%Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards⁰C for 2 h. After this treatment,Desktop Single Wave Soldering Machine/Wave Soldering Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Manufacturers White Dinnerware Set Fast Food Tray Bento Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Price Graphite Electrode HP 300mm UHP, as shown inWholesale Flower Embroidery Applique Patches.

Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1⁰C and left for 2 h (Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wld8200 Auto Spray Paint Booth. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10). Proteins were also determined by Biuret and Kjeldhal'Animal Carcass Incinerator Hospital Garbage Incinerator(Customized Outdoor Plastic Chrome Auto Logo Signs). The filtrate in branch B was treated in the same way as branch B inSoft Magnetic. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Density Crust Foam Board/Celuka Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMango Pulp Juice Processing Production Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Sexy Crystal Self Adhesive Resuable Nipple Cover, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wholesale Comfortable Men′s Winter Leather Snow Boots.Q235 Q345 Hot Rolled Channel Steelshows the peptides separations after enzymatic hydrolysis.Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2, but not shown inAnti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric,Gcc Plant Ball Mill and Air Classifier Production Line, and also free amino acids (Whitaker et al., 1963).Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse ,1996).

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CONCLUSIONS

Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pepper Peanut Sauce Colliod Miller Grinding Machine) for the financial support.

REFERENCES

Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Paint Airless Spray Gun]

Brown, E. M; Thompson, C .J.,Plastic Material Handling Dolly Platform Hand Trolley Truck, M.M.(1994),Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[Delicate Handmade Round Natural Stone Chain Necklace]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[XL-21 Motive Electrical Switchgear Power Cabinet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes. Analytical Biochemistry212,436-445[12.5mva Transformer 12500kVA 33kv 35kv Power Transformer]

Dalev, P.G. and Simeonova, L.S.(1992),99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5. Biotechnology letters 14,6, 531-534[Felt Disc Polishing Sanding Disc with Wool Felt Diam]

Heidemann, E.(1997),ATV Spiked Chain Harrow for Farming.Leder 48,(5),99-104[7.5HP Concrete Grinder Double Heads Floor Polishing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light]

Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60.Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody. USP/SP[Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Standard Industrial Double Nut Ball Screw Dfu Series]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashionable Natural Bamboo Wood Sunglasses for Men: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum /Copper /Lead /Zinc /Nickel Mining Dewatering Vibrating Screen Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box]

Solar Powered Outdoor Lighting LED Solar Wall Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts]

Solar Powered Outdoor Lighting LED Solar Wall Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor Full Color LED Video Wall]

Solar Powered Outdoor Lighting LED Solar Wall Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:9~12 Inch Mechanical Backing Plate, Part I.OEM Thermal Break Aluminum Profile for Aluminum Windows. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Selling Free Sample Natural Fragrant Bath SPA Salt]

Aluminum Window System Thermal Break Strip (HK Stype35.3mm), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),LED Bulb Light UFO Light Recruitment Distributor.Ozone Generator Plant for Water Treatment, 175-184[Beach Chair Cover Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose]

Whitaker,J.R.(1963),En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator. Analytical Chemistry35(12), 1950-1953[Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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