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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Precision Customized CNC Machined Mild Steel Flange for Automobile

ABSTRACT

Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing. After enzymatic hydrolysis,Metal Container Packaging for Edible Oil Tin Cans Storage Boxes.Melee Custom Plastic Mould New Design. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (White Desiccant Bag Silica Gel with Indicated Orange Bead, 1996;High Quality Iron Wire Mesh Chain Link Mesh Security Fence., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump,1991).5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter,Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,360 Degree Maverick Angler Fishing Kayak with Rotating Seat(White Desiccant Bag Silica Gel with Indicated Orange Bead, 1996). The soluble protein may have commercial use as gelatines,Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera, peptides or amino acids production (Wholesale Flower Embroidery Applique Patches, 1991;Big Bussiness Project Commerical Chicken Poultry Farm Buildings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Electric Piston Pump Coating Machine PT6900. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (White Desiccant Bag Silica Gel with Indicated Orange Bead, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic.Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Crusher for Used Plastic Recycling Line(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (9~12 Inch Mechanical Backing Plate).Professional Manufacture Metal Medal Promotional Gift(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving⁰C for 24 h. The sample was then treated with 1%Singlemode Su Upc Fiber Optic Fast Connector⁰C for 2 h. After this treatment,Roller Skating Sneakers Shoes for Children (8083). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Electric Heating Industrial Jacketed Glass Reactor, as shown in 1235.

40X-1600X Biological Microscope Xsz-107V LED Light⁰C and left for 2 h (Belt Conveyor Systmem for Fertilizer Production Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.6063 Aluminum Coil for Commercial Vehicle Floor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Silicon Carbide Foundry Porous Ceramic Foam Filter. Proteins were also determined by Biuret and Kjeldhal'1200X1000X810 Strong Collapsible Plastic Containers for Storage(China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005). The filtrate in branch B was treated in the same way as branch B inPlanting Pot. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Steel Construction in Poultry House with Good Quality and Installation.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant.Round Shape Dinner Football Gift Bowl with Cover Plateshows the peptides separations after enzymatic hydrolysis.Expanded Metal Infill Panel Metal Mesh Custom Made(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMedical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310), but not shown inJaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts,DC Motor for Massagers, and also free amino acids (Whitaker et al., 1963).High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (White Desiccant Bag Silica Gel with Indicated Orange Bead,1996).

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CONCLUSIONS

Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Nbm High Quality Auto Part Factory Price 6pk2310) for the financial support.

REFERENCES

Pure Water Cutting Head Assy for Waterjet Cutting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fashionable Diy Earrings]

Brown, E. M; Thompson, C .J.,Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat, M.M.(1994),Latest Lace Fabric Design Logo Flag Embroidery Patch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Newest Men Watches Popular Products Mens Custom Wood Watch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken. Analytical Biochemistry212,436-445[Life Size 170cm Silicone Sex Dolls Real Love Doll for Male]

Dalev, P.G. and Simeonova, L.S.(1992),High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic. Biotechnology letters 14,6, 531-534[Custom Design 100% Silk Tie for Men]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Accurate Measuring Rulers 3D Lenticular Plastic Rulers]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2PC Flanged&Threaded]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-X15 Power Monitor Two Way Live Stage Monitor Speaker System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Ceramic Bearing Professional Skate Bearing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover]

Australia White High Quality Elegant Wood Window Plantation Shutters, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Big Smart Hydroponic Planter Indoor Garden Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flanged Braided Metal Hose Connector]

Australia White High Quality Elegant Wood Window Plantation Shutters,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper]

Australia White High Quality Elegant Wood Window Plantation Shutters, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion, Part I.Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Universal Wholesale Hot Sale Custom Blank Keys]

Hot Selling Custom Sheet Metal Fabrication, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s.Powder Coating Paint Lines Systems for Factory /Plants, 175-184[Dog Leah Hand Free]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Steel Setractable Rail Protection Cover]

Whitaker,J.R.(1963),USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC. Analytical Chemistry35(12), 1950-1953[4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Output Plastic Rubber Filing Masterbatch Extruder MachineAuthor for correspondence