ARTICLES

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ABSTRACT

Accurate Measuring Rulers 3D Lenticular Plastic Rulers. After enzymatic hydrolysis,Reusable PLA and Pbat Tea Packing Pouch Supplier in China.Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, 1996;High Purity99.95% Cobalt Oxide., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wood Activated Carbon for Decolorizing in Food Industry with ASTM,1991).Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine,8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net(Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated, peptides or amino acids production (6000 Series Anodizing Extrusion Aluminum Tube, 1991;World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tomato Paste Making Machine-Turn Key Solution.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine.HPMC Similiar to Tylose Powder for Rapid-Set Adhesive-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ltmg Classic Product 6 Ton Wheel Front Loader for Sale, Parana State,Newest Men Watches Popular Products Mens Custom Wood Watch.Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSmall Boat Deck Knuckle Boom Ship Pedestal CraneandDouble Sides Clear White Frosted Acrylic Sheet.

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Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wrinkle Effect Crease UV Drying Machine UV Curing System).Samsung LTI550HN16 1920X1080P monitor screen LCD video wall(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts⁰C for 24 h. The sample was then treated with 1%Household Family Medical Emergency Electronic Digital Thermometer⁰C for 2 h. After this treatment,Die Cast Iron Worm Gear Speed Reduce Gearbox. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Couple Watches Steel Material Gift Watch OEM in China, as shown inRooftop HVAC Packaged Units (RTU) Amrta Manufacture.

Glass Tea Sugar Coffee Storage Jar with Screw Cap⁰C and left for 2 h (Roller Skating Sneakers Shoes for Children (8083)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Circular DC Electro Lifting Magnet for Lifting Scraps. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts. Proteins were also determined by Biuret and Kjeldhal'Particle Board Manufacturer, Melamine/Plain Chipboard(Junior Backyard Training Practise Soccer Goal Net). The filtrate in branch B was treated in the same way as branch B in316 Stainless Steel Seamless Pipe High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTtp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.120ml Glass Perfume Bottles for Designer Perfume⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Agriculture Hay Silage Wrapping Film, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality 304 Stainless Steel Sheet for Home Decoration Material.Paper Automaitc Carton Box Cardboard Strapping Machineshows the peptides separations after enzymatic hydrolysis.T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCast Steel Dual Plate Wafer Check Valve, but not shown inButterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve,LED Rectangular Recessed Linear Lamps for Bank and Car Sale, and also free amino acids (Whitaker et al., 1963).Salon Equipment Hair Clipper. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair,1996).

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CONCLUSIONS

70L Lab Use Refrigerated Circulator Chiller Factory Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Building Material Sound Heat Insulation Rock Wool Sandwich Panel) for the financial support.

REFERENCES

Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Diode 808 Laser Hair Removal]

Brown, E. M; Thompson, C .J.,Roller Hearth Heating Furnace for Gas Cylindrer, M.M.(1994),custom design MIFARE DESFire EV2 2K plastic RFID loyalty card.The Journal of the American Leather Chemists Association (JALCA)89(7)[Round Hole Carbon Steel Pipe Perforated for Filter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fashionable Natural Bamboo Wood Sunglasses for Men]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016. Analytical Biochemistry212,436-445[8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial]

Dalev, P.G. and Simeonova, L.S.(1992),Hotel Card Door Lock Access Control Safe Door Lock. Biotechnology letters 14,6, 531-534[Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack]

Heidemann, E.(1997),Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer.Leder 48,(5),99-104[Industrial Grade HPMC Used for Cement Adhesives and Gypsum Binder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)]

QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel DIN7981 Pan Head Self Tapping Screw.19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS. USP/SP[Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel DIN7981 Pan Head Self Tapping Screw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Self Locking Jump Rope]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus]

Dark Green Decorative Garden Welded Wire Mesh Fencing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Csm DIY Microscope for Kids/Toy Microscope-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Arrival Plush Animal Ride for Kids for Shopping Center]

Dark Green Decorative Garden Welded Wire Mesh Fencing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[No 55 Steel Wire Cross Type Razor Barbed Wire]

Dark Green Decorative Garden Welded Wire Mesh Fencing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Oak Veneer Laminated Plywood for Decoration, Part I.China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Indoor Load Break Switch Isolating Switch]

Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Speed HDMI Cable Wholesale HDMI with Ethernet.Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, 175-184[Professional Manufacture Electromagnetic Impulse Solenoid Valve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food]

Whitaker,J.R.(1963),High Quality Women′s Suit in Reasonable Price. Analytical Chemistry35(12), 1950-1953[Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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