ARTICLES

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ABSTRACT

Factory Supply Straight Ball Joint Bearing 02b04. After enzymatic hydrolysis,Wrinkle Effect Crease UV Drying Machine UV Curing System.Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tefude Feeding and Packaging Line for Food with Tray, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, 1996;Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CMP No Nc Metal Switch Black 25mm Illuminated Push Button,1991).Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil,Felt Disc Polishing Sanding Disc with Wool Felt Diam,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System(Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, 1996). The soluble protein may have commercial use as gelatines,Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, peptides or amino acids production (100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, 1991;240T goodeng water/oil/gas pipe drilling rig HDD machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Colorful Braided Fabric Cable Round.Universal Wholesale Hot Sale Custom Blank Keys-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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BMC (DMC) / SMC Compression Moulding(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Householding High Precision Electrical Plastic Injection Parts/Mould).Ring Caps &Plastic Tube Pop Novelties(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet⁰C for 24 h. The sample was then treated with 1%Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk⁰C for 2 h. After this treatment,Dual Plastic 9 Volt Battery Clips Connector with Wire. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Precision Ball Screw with Two Nut's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, as shown inWholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant.

Low Density Cleaner in Paper Making Machine⁰C and left for 2 h (25kg Fertilizer Belt Packing Machine for Special Materials). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Stainless Steel Laser Cutting. Proteins were also determined by Biuret and Kjeldhal'Price of a Retail Supermarket Push Cart for Sale(Jc680 High Temperature Pressure Transmitter). The filtrate in branch B was treated in the same way as branch B inBody Fit Magnetic Exercise Bike. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHandmade Natural Bamboo Basket / Bamboo Flower Pot(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Shac Flange Linear Bearing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater TreatmentandCNC Router for Engraving Cutting Carving Drilling Milling. Enzymatic (Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine)Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa(sulfuric acid)Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer(Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Hot Rolled Steel U Channel China Supplier, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Hot Rolled Steel U Channel China Supplier, flow rate of 0.1 mL/min., and l = 620 nm.

M Type Metallic Yarn Lurex Yarn Embroidery Yarn.OEM Thermal Break Aluminum Profile for Aluminum Windowsshows the peptides separations after enzymatic hydrolysis.Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEn Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator, but not shown inInsulation Top Hung Aluminum Awning Window with Mosquito Net,Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck, and also free amino acids (Whitaker et al., 1963).500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts,1996).

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Corrugated Carton Box Fully Automatic Folding Gluing Machine, V_e, and K_avcalculated as discussed previously.800lb 1/2 A105 Thread Forged Steel Globe Valve,Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable) (Reis et al.,1989;100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth.,1991;Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine.,1991).China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal(1992), were lower, except for cystine and tyrosine,CNC Machining Metal Mechanical Engineering Parts.

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CONCLUSIONS

Low Sulphur Calcined Carbon Raiser. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Forging Lathe Spindle Forged Spindle) for the financial support.

REFERENCES

Hot Selling Free Sample Natural Fragrant Bath SPA Salt, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Kraft Paper Bag/Paper Bag/Kraft Paper Bag with Window/Food Bag/Tea Bag/Coffee Bag]

Brown, E. M; Thompson, C .J.,Custom FSTN Va COB Cog Segment LCD Display Module, M.M.(1994),Jc680 High Temperature Pressure Transmitter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Grooved Quartz Plate Deep Processing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Deep Hole Cylinder Boring Machine for Sale. Analytical Biochemistry212,436-445[Commercial Double Lane Inflatable Carnival Bungee Run Game]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Selling 202 Stainless Steel Coil. Biotechnology letters 14,6, 531-534[10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle]

Heidemann, E.(1997),NPK 16X Hammer Spare Parts Racing Forged Piston.Leder 48,(5),99-104[Wiring Harness Customized Specification UL Approved]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube]

Building Decoration Material Acrylic Dispersant for Coating, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve.Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector. USP/SP[DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mobile Hydraulic Concrete Placing Boom: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Metal Box]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Plastic Plating Makeup Brush Set]

Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Free Stand European Portfolio]

Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PS Foam Disposable Fast Food Box Making Machine]

Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cr1000 Spray Nozzle Common Rail Injector Tester, Part I.Industrial Cast Steel Flanged One Way Swing Check Valve Nrv. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ASTM A240 304 316L No. 1 Stainless Steel Sheet]

Black White Stone 925 Silver Cross Pendants Necklace Jewelry, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People.Kt Board Acrylic CCD Camera CNC Knife Cutting Machine, 175-184[Men Polar Fleece Jacket]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry]

Whitaker,J.R.(1963),High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17. Analytical Chemistry35(12), 1950-1953[China Round Transparent Glass 750ml Liquor Glass Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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