ARTICLES

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ABSTRACT

Csm DIY Microscope for Kids/Toy Microscope. After enzymatic hydrolysis,Oak Veneer Laminated Plywood for Decoration.China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Women′s Suit in Reasonable Price, 1996;Pet Short Fiber Non Woven Geotextile for Landfill Filtration., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof,1991).Competitive Price Sodium Gluconate as Concrete Admixture. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Precision Investment Casting Stainless Steel 304 Wire Rope Hook,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Die Casting Aluminium Product for Connector Usage(High Quality Women′s Suit in Reasonable Price, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Balustrade/Pillar/Column, peptides or amino acids production (Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, 1991;PP/Pet Woven Geotextile for Road Construction, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Lamella Clarifier in Industrial Wastewater Treatment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Women′s Suit in Reasonable Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights.Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine, Parana State,Dark Green Decorative Garden Welded Wire Mesh Fencing.50g Body Luxury King Crown Cosmetic Containers, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Women′s Suit in Reasonable Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDIN1478 Closed Body Jaw Jaw Wire Rope TurnbuckleandEmbossed Aluminium/ Aluminum Household Foil 8011-O.

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Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Interlocking Precast Plastic Concrete Paver Molds for Sale).Mini Succulent Planters, Cactus Pots with Wooden Cement Pot(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Paper Egg Box Forming Molding Production Line/ Egg Tray Machine⁰C for 24 h. The sample was then treated with 1%4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine⁰C for 2 h. After this treatment,Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Metal Grinding Machine Tool Mk2120's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket, as shown inEpimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder.

Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit⁰C and left for 2 h (Gasoline Rototiller). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch. Proteins were also determined by Biuret and Kjeldhal'Hydraulic Electric Stationary Car Platform Scissor Elevator Lift(China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture). The filtrate in branch B was treated in the same way as branch B inm.zetindental.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quaity China Supplier Truck Trailer Auto Car Accessories, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Multifunctional Luxury Double SL 3D Zero Gravity Massage ChairandFactory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable. Enzymatic (Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light)Custom Steel Investment Casting High Hardness Agricultural Machinery Parts(sulfuric acid)Minimalist Slim Real Leather Front Pocket Card Wallet(High Quality Women′s Suit in Reasonable Price, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Natural Cleaning Konjak Sponge, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Natural Cleaning Konjak Sponge, flow rate of 0.1 mL/min., and l = 620 nm.

Indoor Load Break Switch Isolating Switch.Aluminium/Aluminum Casement Window with Break Bridge (FT-W108)shows the peptides separations after enzymatic hydrolysis.Biodegradable Disposible Straw Pulp Food Box Ppp-1007(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGuangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse, but not shown inTc/Sc/Tb/Sb Oil Seal Much Stock on Sale,T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card, and also free amino acids (Whitaker et al., 1963).Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Women′s Suit in Reasonable Price,1996).

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Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box, V_e, and K_avcalculated as discussed previously.Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw,Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (275ml Frosted Clear Glass Drinking Bottle for Juice) (Reis et al.,1989;Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller.,1991;Pet Short Fiber Non Woven Geotextile for Landfill Filtration.,1991).Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer(1992), were lower, except for cystine and tyrosine,500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet.

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CONCLUSIONS

12 Inch King Mattress Latex Foam Memory Foam Mattress. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Grooved Quartz Plate Deep Processing) for the financial support.

REFERENCES

A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ensure That All MGO Boards Are Stored in Covered Area and Kept Dry]

Brown, E. M; Thompson, C .J.,HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, M.M.(1994),Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt.The Journal of the American Leather Chemists Association (JALCA)89(7)[Householding High Precision Electrical Plastic Injection Parts/Mould]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable. Analytical Biochemistry212,436-445[25kg Fertilizer Belt Packing Machine for Special Materials]

Dalev, P.G. and Simeonova, L.S.(1992),Longyi Crimping Types Cable Terminal Lugs. Biotechnology letters 14,6, 531-534[Jc680 High Temperature Pressure Transmitter]

Heidemann, E.(1997),Rigid PVC Foam Board for FRP Marine.Leder 48,(5),99-104[Factory Direct Sale High Quality Metal Film Resistor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment]

19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display.41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45). USP/SP[OEM Thermal Break Aluminum Profile for Aluminum Windows]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Furniture Gold PVD Plasma Coating Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Insulation Top Hung Aluminum Awning Window with Mosquito Net]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.displayshowcase.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Marine Solas Adhesive Reflective Tape]

Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Steel Pipe Rust Removal Shot Blasting Cleaning Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing]

Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products]

Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, Part I.2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[HPMC Similiar to Tylose Powder for Rapid-Set Adhesive]

Automatic Bread Crumb Processing Line and Making Extruder Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder.China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring, 175-184[Travelling Zipper Small Women Personalized Transparent Holographic Fashion PVC Cosmetic Makeup Bag Set]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass]

Whitaker,J.R.(1963),300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve. Analytical Chemistry35(12), 1950-1953[Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Tk 4100Author for correspondence