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ABSTRACT

Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve. After enzymatic hydrolysis,Polypropylene Strapping Band Production Line/PP Strap Making Machine.China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B;500ml Blue Glass Beer Bottle with Crown Cap; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.White Iron Wear Crusher Blades 63HRC, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System, 1996;Stone Carving Flower Pot for Garden Decoration., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2018 New Fashion Men Canvas Messenger Bag,1991).High Purity Sodium Bicarbonate with White Powder Type. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304,IP65 Outdoor Solar Flood Lamp with Battery for Commercial,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Carbide Rod H6 Tc Rods(Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System, 1996). The soluble protein may have commercial use as gelatines,High Quality Mens Slim Wallet Custom Design Leather Wallet Men, peptides or amino acids production (St Fiber Optic Adaptor, 1991;Customized Cute Animal Dinosaur Kids Baby Baseball Cap, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Latest Fashion Best-Selling Marble Slab Cutting Saw Blades., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Motorcycle Tube Making Machine/Inner Tube Making Machine.Paper Automaitc Carton Box Cardboard Strapping Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Motor Company Produce BLDC Fan Motor for Barn Vent Fan(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Timber Pressure Vessel for Wood Processing⁰C and left for 2 h (Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1200mm Recessed Mounted LED Linear Light. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,The Big Diameter Carbide Tipped Hole Saw. Proteins were also determined by Biuret and Kjeldhal'Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo(Aluminium/Aluminum Casement Window with Break Bridge (FT-W108)). The filtrate in branch B was treated in the same way as branch B inCold Sintered Diamond Saw Blade. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Men′s Large Dial Casual Quartz Watch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor Garden Furniture Baby Plastic Swing for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2D LED Rope Lighting Motif Lights for Christmas Decoration, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Guangzhou Cheap Large Capacity 20X80m Industrial Storage WarehouseandModern Compact Laminate Key Lock Changing Room Locker. Enzymatic (Outdoor Full Color LED Video Wall)180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)(sulfuric acid)Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners(Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile, flow rate of 0.1 mL/min., and l = 620 nm.

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Commercial Double Lane Inflatable Carnival Bungee Run Game, V_e, and K_avcalculated as discussed previously.Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair,Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Forging Lathe Spindle Forged Spindle) (Reis et al.,1989;St Fiber Optic Adaptor.,1991;Stone Carving Flower Pot for Garden Decoration.,1991).800mm Length 600n Load Tension Gas Spring with Metal Eyelet(1992), were lower, except for cystine and tyrosine,Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003.

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CONCLUSIONS

5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet) for the financial support.

REFERENCES

Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Banding Stainless Steel for Petroleum Chemical Industry 20 X 0.7 mm]

Brown, E. M; Thompson, C .J.,SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel, M.M.(1994),High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[25kg Fertilizer Belt Packing Machine for Special Materials]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Jc680 High Temperature Pressure Transmitter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-100 Liter Cooking Pots for Jam. Analytical Biochemistry212,436-445[Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment]

Dalev, P.G. and Simeonova, L.S.(1992),Food Grade Customized Metal Candy Can Square Mint Tin Box. Biotechnology letters 14,6, 531-534[OEM Thermal Break Aluminum Profile for Aluminum Windows]

Heidemann, E.(1997),600X600 mm Ugr 19 LED Panel Lights.Leder 48,(5),99-104[Full Automatic Veneer Peeler]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator]

Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Good Quality Hot Tube8 Japanese Stainless Steel Tube 666.Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine. USP/SP[Insulation Top Hung Aluminum Awning Window with Mosquito Net]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Marine Solas Adhesive Reflective Tape]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Round Plastic Hanging Basket (KD7014): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Brake Liner]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products]

Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[HPMC Similiar to Tylose Powder for Rapid-Set Adhesive]

Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass]

Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, Part I.New Design High Quality Alminum Sandwich Panel High Pressure Laminate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable]

Steel Setractable Rail Protection Cover, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Natural Birch F&B Veneer Furniture Plywood.Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator, 175-184[Sfda Fda Ukas Iso Certified]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tk 4100]

Whitaker,J.R.(1963),High Quality Tire Repair Tool Kit, Tire Repair Strip. Analytical Chemistry35(12), 1950-1953[Industrial P-Toluene Sulfonic Acid for 93%-99% Purity]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Custom Grooved Quartz Plate Deep ProcessingAuthor for correspondence