ARTICLES

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ABSTRACT

Fea040b New Arrival Airplane Seat Belt Extender. After enzymatic hydrolysis,SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving.Square Shape Glass Candle Holder for One Piece Tealight Candle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Easy Foldable Kids Walking Chair Toys Baby Walker, 1996;Men V-Neck Longline T-Shirt with Color Block Ribbed Trims., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener,1991).Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Outdoor Multifunctional Solar Power Flashlight (SYSG-517),Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass(Easy Foldable Kids Walking Chair Toys Baby Walker, 1996). The soluble protein may have commercial use as gelatines,High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer, peptides or amino acids production (FC Bare Fiber Optic Adapter for Network, 1991;3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cattle Corpse Cremation Burning Stove., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Easy Foldable Kids Walking Chair Toys Baby Walker, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment.Professional Stainless Steel Biological Fermentor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box).900-1800MHz GSM PCB Antenna, 3m Adhesive GSM Built-in PCB Antenna(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe⁰C for 24 h. The sample was then treated with 1%Plastic Flange Housing Ucf310-115 for Electric Hand Tools⁰C for 2 h. After this treatment,Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester, as shown inFactory Price 4X6 Acrylic Magnetic Photo Frame.

Tct Circular Saw Blade &Diamond Saw Blade⁰C and left for 2 h (Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Lifting Thread with Needle for Plastic Surgery. Proteins were also determined by Biuret and Kjeldhal'Methyl Cyclohexane Methylcyclohexane, Organic Chemicsls(Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect). The filtrate in branch B was treated in the same way as branch B inWoven Carpet Exhibition Carpet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fudan Compatible Card 1k RFID Card 13.56MHz. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHappy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations, pH 3.25., mobile phase B: buffer solution of 0.01 mol/AC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding.Biodegradable Disposible Straw Pulp Food Box Ppp-1007shows the peptides separations after enzymatic hydrolysis.24 Hours Self-Service Water ATM Machine Automatic RO Water Vending Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNPK 16X Hammer Spare Parts Racing Forged Piston, but not shown inAir Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder,Professional Plastic Plating Makeup Brush Set, and also free amino acids (Whitaker et al., 1963).Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Easy Foldable Kids Walking Chair Toys Baby Walker,1996).

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Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer, V_e, and K_avcalculated as discussed previously.Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace,5 Star European Antique Hotel Furniture. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (130kw 7 Degrees Scroll Type Industrial Water Chiller) (Reis et al.,1989;FC Bare Fiber Optic Adapter for Network.,1991;Men V-Neck Longline T-Shirt with Color Block Ribbed Trims.,1991).4.0MP Ahd Tvi CVBS HD CCTV Camera Module(1992), were lower, except for cystine and tyrosine,Active Demand Plastic 65 Liter Laundry Basket Mould.

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Methyl Cyclohexane Methylcyclohexane

CONCLUSIONS

Organic Fertilizer Compost Turning Machine, Compost Turner Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (16 Spindle Lace High Speed Braiding Machine Textile Machinery) for the financial support.

REFERENCES

Insulation Top Hung Aluminum Awning Window with Mosquito Net, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Polyester Trump Flag]

Brown, E. M; Thompson, C .J.,4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger, M.M.(1994),Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sinotruk-HOWO 6X4 Tractor Truck]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sell Data USB Connector Type C 12 Pin Terminal Block. Analytical Biochemistry212,436-445[Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light]

Dalev, P.G. and Simeonova, L.S.(1992),Unique Handle 24mm Water Bottle Caps. Biotechnology letters 14,6, 531-534[Customized Stainless Steel Laser Cutting]

Heidemann, E.(1997),Stainless Steel Band Cable Ties Wire Strap Steel Banding.Leder 48,(5),99-104[etonsmtauto.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Household Family Medical Emergency Electronic Digital Thermometer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Boat Aluminum Pipe 5083 Marine Used Aluminum Tube]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Camping Picnic Blanket]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Multi Sensor Nilitary Surveillance Thermal Imaging Security camera]

15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Cube Ice Maker Parts / Ice Machines Maker Cube]

15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB]

15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production, Part I.Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning]

High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector.New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap, 175-184[New Shacman F3000 6X4 Truck Trailer Head]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[H365 Emas Chainsaw Spares Cylinder Kits]

Whitaker,J.R.(1963),Double Door Heated Holding Carts. Analytical Chemistry35(12), 1950-1953[IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Purity99.95% Cobalt OxideAuthor for correspondence