ARTICLES

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ABSTRACT

Metal Detector for Gold, Silver, etc Underground Metel Detector. After enzymatic hydrolysis,Hing Quality Rubber O Ring in Motor High Pressure.Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor;PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc, 1996;Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Free Shipping Wholesale Peruvian Human Hair Weave,1991).Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter,Printing Pure Color Women Fitness Wear,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve(Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc, 1996). The soluble protein may have commercial use as gelatines,Polypropylene Strapping Band Production Line/PP Strap Making Machine, peptides or amino acids production (China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor, 1991;12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;500ml Blue Glass Beer Bottle with Crown Cap., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.White Iron Wear Crusher Blades 63HRC.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stone Carving Flower Pot for Garden Decoration.2018 New Fashion Men Canvas Messenger Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Purity Sodium Bicarbonate with White Powder Type(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

St Fiber Optic Adaptor, Parana State,Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h.Customized Cute Animal Dinosaur Kids Baby Baseball Cap, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theASTM A240 304 316L No. 1 Stainless Steel SheetandHot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry.

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Latest Fashion Best-Selling Marble Slab Cutting Saw Blades(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera).Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover⁰C for 24 h. The sample was then treated with 1%Motorcycle Tube Making Machine/Inner Tube Making Machine⁰C for 2 h. After this treatment,Paper Automaitc Carton Box Cardboard Strapping Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Motor Company Produce BLDC Fan Motor for Barn Vent Fan's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB, as shown inVertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box.

1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank⁰C and left for 2 h (Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10. Proteins were also determined by Biuret and Kjeldhal'Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)(15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s). The filtrate in branch B was treated in the same way as branch B inAnti-Slip TPU or PVC Tactile Indicator Paving Tile. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rht-060ws Soda Process Water Cooled Screw Type Water Chiller. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale.Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernelshows the peptides separations after enzymatic hydrolysis.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOutdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool, but not shown in8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera,Fashion Men′s Large Dial Casual Quartz Watch, and also free amino acids (Whitaker et al., 1963).PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc,1996).

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Modern Compact Laminate Key Lock Changing Room Locker, V_e, and K_avcalculated as discussed previously.Outdoor Full Color LED Video Wall,180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Lamella Clarifier in Industrial Wastewater Treatment) (Reis et al.,1989;China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor.,1991;Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom.,1991).Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners(1992), were lower, except for cystine and tyrosine,Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile.

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CONCLUSIONS

Quick Connect Camlock and Grooved Couplings Quick Fitting Type D. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Carbide Rod H6 Tc Rods) for the financial support.

REFERENCES

High Quality White Calcined Alumina Powder for Ceramic Industry, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Rebar Tying Machine]

Brown, E. M; Thompson, C .J.,500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine, M.M.(1994),100kw Cummins Diesel Generator Set.The Journal of the American Leather Chemists Association (JALCA)89(7)[Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Water Chiller Air Cooled for Cooling Beer Refrigerating]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle. Analytical Biochemistry212,436-445[Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants]

Dalev, P.G. and Simeonova, L.S.(1992),Luxury Hotel Furniture Antique Design Marble Side Table. Biotechnology letters 14,6, 531-534[2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace]

Heidemann, E.(1997),Ampoule Sealer Machine Hydrogen Oxygen Gas Generator.Leder 48,(5),99-104[Factory Price Passport Holder Credit Card Protector RFID Blocking Sleeve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Zs Round Vibrating Sieve]

EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Commercial Double Lane Inflatable Carnival Bungee Run Game.Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair. USP/SP[Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ 20t]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Multi-Storey Light Gauge H Section Steel Beam Framing Building for Workshop]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Highest Precision Plastic Injection Molding Part for Consumer Product (LW-01053)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[360 Degree Maverick Angler Fishing Kayak with Rotating Seat]

800mm Length 600n Load Tension Gas Spring with Metal Eyelet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box]

800mm Length 600n Load Tension Gas Spring with Metal Eyelet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor Playground Plastic Slide with Swing and Basketry]

800mm Length 600n Load Tension Gas Spring with Metal Eyelet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, Part I.Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Big Dimension UHP Graphite Electrodes for Steel Making]

SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale.100 Liter Cooking Pots for Jam, 175-184[Auto Type Vacuum Membrane Press Machine for Door/Wooden Product Coating]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Promotion Snack Children School Lunch Bag Cooler Bag]

Whitaker,J.R.(1963),Food Grade Customized Metal Candy Can Square Mint Tin Box. Analytical Chemistry35(12), 1950-1953[Neodymium Magnet with RoHS ISO 16499 Approved]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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