ARTICLES

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ABSTRACT

Fused Silica Quartz Lump 5-50mm for Refractory Materials. After enzymatic hydrolysis,Cold Drawn D2 H13 Alloy Steel Turning Roll.Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Colorful 100LED Copper String Light Outdoor LED, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (H365 Emas Chainsaw Spares Cylinder Kits, 1996;PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (A4 Diploma Covers Wholesale with Silver Foil,1991).OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap,120L 240L Oil Universal Chemical Spil Absorbent Kits,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse(H365 Emas Chainsaw Spares Cylinder Kits, 1996). The soluble protein may have commercial use as gelatines,High-Tower Compound NPK Fertilizer 15-15-15, peptides or amino acids production (20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21, 1991;8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hybrid Inverter Combined MPPT Controller 1-12kw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (H365 Emas Chainsaw Spares Cylinder Kits, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Newest Men Watches Popular Products Mens Custom Wood Watch.Accurate Measuring Rulers 3D Lenticular Plastic Rulers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Reusable PLA and Pbat Tea Packing Pouch Supplier in China(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, Parana State,Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs.High Purity99.95% Cobalt Oxide, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (H365 Emas Chainsaw Spares Cylinder Kits, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAd-L13 Black Perfume Plastics Vial Bottle 10mlandHot Sale High-Order Elasticty Girls Sports Wear Yaga Suit.

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Wood Activated Carbon for Decolorizing in Food Industry with ASTM(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV).Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine⁰C for 24 h. The sample was then treated with 1%8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts⁰C for 2 h. After this treatment,Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)6000 Series Anodizing Extrusion Aluminum Tube, as shown inPlastic Material Handling Dolly Platform Hand Trolley Truck.

World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J)⁰C and left for 2 h (500ml Blue Glass Beer Bottle with Crown Cap). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tomato Paste Making Machine-Turn Key Solution. Proteins were also determined by Biuret and Kjeldhal'Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper(LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip). The filtrate in branch B was treated in the same way as branch B inm.baiyipackage.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%HPMC Similiar to Tylose Powder for Rapid-Set Adhesive. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNPT Standard Malleable Iron Pipe Fitting Hexagon Nipple(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Selling Solidwood Viola Wholesale Price Viola⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Building Material Sound Heat Insulation Rock Wool Sandwich Panel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Chromium Oxide Green 99% for Construction and PlasticsandLtmg Classic Product 6 Ton Wheel Front Loader for Sale. Enzymatic (Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror)Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar(sulfuric acid)Samsung LTI550HN16 1920X1080P monitor screen LCD video wall(H365 Emas Chainsaw Spares Cylinder Kits, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts, flow rate of 0.1 mL/min., and l = 620 nm.

Household Family Medical Emergency Electronic Digital Thermometer.Fashion Men′s Large Dial Casual Quartz Watchshows the peptides separations after enzymatic hydrolysis.Die Cast Iron Worm Gear Speed Reduce Gearbox(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply, but not shown inOEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate,19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting, and also free amino acids (Whitaker et al., 1963).High Quality Couple Watches Steel Material Gift Watch OEM in China. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (H365 Emas Chainsaw Spares Cylinder Kits,1996).

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Compounding Pelletizer Line

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Particle Board Manufacturer, Melamine/Plain Chipboard, V_e, and K_avcalculated as discussed previously.CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part,Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Laser Cut Sheet Metal Fiber Laser Cutting Machine) (Reis et al.,1989;20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21.,1991;PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg.,1991).Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert(1992), were lower, except for cystine and tyrosine,Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps.

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CONCLUSIONS

120ml Glass Perfume Bottles for Designer Perfume. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industrial P-Toluene Sulfonic Acid for 93%-99% Purity) for the financial support.

REFERENCES

Agriculture Hay Silage Wrapping Film, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.asiamzsteel.com]

Brown, E. M; Thompson, C .J.,Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer, M.M.(1994),Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane.The Journal of the American Leather Chemists Association (JALCA)89(7)[Building Material of Granite Ceramic Wall Tile for Wall Decoration]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic Flange Housing Ucf310-115 for Electric Hand Tools]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-16oz Insulated Cardboad Cup Sleeve. Analytical Biochemistry212,436-445[Rock Tunnel Boring Machine for Natural Gas Pipeline]

Dalev, P.G. and Simeonova, L.S.(1992),Large Outdoor Simple Gazebo Beer Festival Marquee Tent. Biotechnology letters 14,6, 531-534[High Purity Sodium Bicarbonate with White Powder Type]

Heidemann, E.(1997),HOWO Tipper Truck Price with Manufacture Factory Price.Leder 48,(5),99-104[Vietnam Cambodia Thailand Asphalt Shingle Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lamella Clarifier in Sewage Tretament Plant for Water Purification]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Interlocking Precast Plastic Concrete Paver Molds for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[on-Board]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder]

LED Rectangular Recessed Linear Lamps for Bank and Car Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Salon Equipment Hair Clipper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Gasoline Rototiller]

LED Rectangular Recessed Linear Lamps for Bank and Car Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture]

LED Rectangular Recessed Linear Lamps for Bank and Car Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flat Conveyor Belt Steel Idler Frame for Roller, Part I.LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair]

Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Durable TPR Pet Molar Toy with Colorful Rope.Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring, 175-184[Automotive Spray Booth Oven Bake Booth Paint Spray Booth for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminium/Aluminum Casement Window with Break Bridge (FT-W108)]

Whitaker,J.R.(1963),Chromium Oxide Green 99% for Construction and Plastics. Analytical Chemistry35(12), 1950-1953[Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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