ARTICLES

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ABSTRACT

20W Solar Street Light with Lens Motion Sensor 2 Years Warranty. After enzymatic hydrolysis,CNC Machined Power Transmission Shaft Part.Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Good Price Channel Steel Used for Machinery;Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, 1996;Factory Direct Sale Patent up to Down Rubber Tile Curing Press., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China,1991).Real Leather Wallet Mens Designer Leather Wallets. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338),Full Port Small Automatic Electronic Plumbing Ball Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,(Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, 1996). The soluble protein may have commercial use as gelatines,Cleanroom White PVC Dotted Anti-Static Gloves, peptides or amino acids production (High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, 1991;Marine Solas Adhesive Reflective Tape, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ASTM A240 304 316L No. 1 Stainless Steel Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰12V 24V 220V Digital Temperature Controller.Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:25kg Fertilizer Belt Packing Machine for Special Materials(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rpl Straight Socket and Plugs Male Thread(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable).46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Metal Stamping Stainless Steel Parts with CNC Machining⁰C for 24 h. The sample was then treated with 1%Ss Wafer Type Check Valve Dn32 Dn40 Dn50⁰C for 2 h. After this treatment,2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera, as shown inHot Sell Data USB Connector Type C 12 Pin Terminal Block.

WPC Wood Plastic Board Equipment Extruder Machine⁰C and left for 2 h (50g Body Luxury King Crown Cosmetic Containers). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Neoprene Elbow Support. Proteins were also determined by Biuret and Kjeldhal'Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules(2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards). The filtrate in branch B was treated in the same way as branch B inJacket Making Handle Forming Akr PC 850 Price High Speed Flexo Printing Recycle Automatic Double Head Paper Cup Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CNC Produce Medical Machining Custom Metal Milling.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Multi Sensor Nilitary Surveillance Thermal Imaging Security camera. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Metal Stamping Welding Spare Parts for Farming(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/275ml Frosted Clear Glass Drinking Bottle for Juice, pH 3.25., mobile phase B: buffer solution of 0.01 mol/EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Light Blue PVC Plastic Sheet and PVC Film.High Precision Mold Manufacturer Plastic Injection Mouldshows the peptides separations after enzymatic hydrolysis.Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWPC Wood Plastic Board Equipment Extruder Machine, but not shown in40X-1600X Biological Microscope Xsz-107V LED Light,Metric Female Cone Hydraulic Hose Fitting, and also free amino acids (Whitaker et al., 1963).Hot Sale Dental Material Dental PMMA Block From China. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount,1996).

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CONCLUSIONS

Fea040b New Arrival Airplane Seat Belt Extender. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby) for the financial support.

REFERENCES

SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Durable Steel and Wood University Laboratory Island Bench]

Brown, E. M; Thompson, C .J.,Square Shape Glass Candle Holder for One Piece Tealight Candle, M.M.(1994),1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Die Casting Zinc Alloy Badge Use for Car]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Medical Autoclavable Surgical Thoracoscopy Trocar. Analytical Biochemistry212,436-445[Ampoule Sealer Machine Hydrogen Oxygen Gas Generator]

Dalev, P.G. and Simeonova, L.S.(1992),DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge. Biotechnology letters 14,6, 531-534[55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk]

Heidemann, E.(1997),Easy Foldable Kids Walking Chair Toys Baby Walker.Leder 48,(5),99-104[Customized Microwave Oven Membrane Switch Keyboard]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[IS Type Cast Iron Chemical Industrial Pump]

Permanent Suspended Magnetic Separator for Belt Conveyor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Men V-Neck Longline T-Shirt with Color Block Ribbed Trims.Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener. USP/SP[Starter Motor 0001367034 12V 3KW 9T for Mercedes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[White Mosquito Clothing For Photographers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[M Type Metallic Yarn Lurex Yarn Embroidery Yarn]

Outdoor Multifunctional Solar Power Flashlight (SYSG-517), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve]

Outdoor Multifunctional Solar Power Flashlight (SYSG-517),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector]

Outdoor Multifunctional Solar Power Flashlight (SYSG-517), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass, Part I.High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes]

FC Bare Fiber Optic Adapter for Network, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory.Cattle Corpse Cremation Burning Stove, 175-184[Cheering Commercial Counter-Top Hot Warmer Display Cabinet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts]

Whitaker,J.R.(1963),L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale. Analytical Chemistry35(12), 1950-1953[OEM Eye Anti-Aging Serum]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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