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^IICNPq/RHAE/biotechnology-Acid Water Pulse Output Liquid Orifice Type Flow Meter. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware

ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector, 1996;New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Double Door Heated Holding Carts,1991).2019 New Water Quality TDS Test. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Top Furniture Hotel Dining Furniture Metal Hotel Table Chair,Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Machinery Parts Aeroplane Engine CNC Milling(Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector, 1996). The soluble protein may have commercial use as gelatines,Aws E7018 Low Carbon Steel Welding Electrode, peptides or amino acids production (Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting, 1991;Electrical Bike Waterproof Power Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Painted G80 Cargo Lashing Chain with Hooks.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Feeding Customized Multi Layers Shirts Cutting Machine.Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Portable Powerful Charger with Dual Pd Charger and Type-C Charger(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Universal Wholesale Hot Sale Custom Blank Keys).High Speed Steel Mill Roll with High Quality(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer⁰C for 24 h. The sample was then treated with 1%2017 China Hot Sale Water Screen Movie Fountain⁰C for 2 h. After this treatment,Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip, as shown inSteel Setractable Rail Protection Cover.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChristmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cross Hex Flange Screw, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sludge Oil Separation Centrifuge.Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Materialshows the peptides separations after enzymatic hydrolysis.8011 Aluminum Foil for Cap Used(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in600X600 mm Ugr 19 LED Panel Lights, but not shown in8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts,Pepper Peanut Sauce Colliod Miller Grinding Machine, and also free amino acids (Whitaker et al., 1963).Gasoline Rototiller, Cultivator, Tiller Model Gtm-6. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector,1996).

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CONCLUSIONS

Best Price Graphite Electrode HP 300mm UHP. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse) for the financial support.

REFERENCES

Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rubber Compression Molded Products Hydraulic Vulcanizing Press Machine Curing Vulcanizer]

Brown, E. M; Thompson, C .J.,Wld8200 Auto Spray Paint Booth, M.M.(1994),Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10).The Journal of the American Leather Chemists Association (JALCA)89(7)[Gcc Plant Ball Mill and Air Classifier Production Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Watermrk Drop in Bathroom Basin Sink]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Animal Carcass Incinerator Hospital Garbage Incinerator. Analytical Biochemistry212,436-445[Pure Water Cutting Head Assy for Waterjet Cutting Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material. Biotechnology letters 14,6, 531-534[500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter]

Heidemann, E.(1997),High Density Crust Foam Board/Celuka Board.Leder 48,(5),99-104[gzyezhifurniture]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2018 New Fashion Men Canvas Messenger Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Cake Table]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2D LED Rope Lighting Motif Lights for Christmas Decoration]

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Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016]

Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Candy Box Laser Marking Engraving Machine Scm2000 180W 250W, Part I.130kw 7 Degrees Scroll Type Industrial Water Chiller. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Metal Cuting CNC Plasma Cutting Machine (DW1325)]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Mini Bag Spices Small Salt Packing Packaging Machine]

Whitaker,J.R.(1963),Wholesale Comfortable Men′s Winter Leather Snow Boots. Analytical Chemistry35(12), 1950-1953[SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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