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ABSTRACT

Hot Sale 840 Type PPGI Color Steel Roofing Sheets. After enzymatic hydrolysis,FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector.CATV Indoor Optical Receiver FTTH. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Best Sell 16L Square Dustbin Frame Mould;Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml, 1996;PVDF Exterior Design Curved Aluminum Panel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Neodymium Magnet with RoHS ISO 16499 Approved,1991).Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price,High Pulling Force Rotatable Rare Earth Crane Magnet Hook,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products(Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml, 1996). The soluble protein may have commercial use as gelatines,Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women, peptides or amino acids production (High-Performance Thermal Break Profiles Aluminium for Doors and Windows, 1991;Design Luxury Moon Cake Box with Hot Stamp Logo, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Standard Industrial Double Nut Ball Screw Dfu Series., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized Logo Outdoor Hanging Swinging Camping Hammock.EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor, Parana State,Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts.PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM Metal Stamping Stainless Steel Parts with CNC Machiningand 49.

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Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller).Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom⁰C for 24 h. The sample was then treated with 1%Free Shipping Wholesale Peruvian Human Hair Weave⁰C for 2 h. After this treatment,Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Printing Pure Color Women Fitness Wear, as shown inChina Factory Light Soda Ash /Sodium Carbonate.

Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve⁰C and left for 2 h (Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Polypropylene Strapping Band Production Line/PP Strap Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor. Proteins were also determined by Biuret and Kjeldhal'12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B(Csm DIY Microscope for Kids/Toy Microscope). The filtrate in branch B was treated in the same way as branch B inFlexible Cornering 48V Electric Carving Vehicle 3 Point Cambering E-Scooter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,500ml Blue Glass Beer Bottle with Crown Cap.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%White Iron Wear Crusher Blades 63HRC. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2018 New Fashion Men Canvas Messenger Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Purity Sodium Bicarbonate with White Powder Type, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)andIP65 Outdoor Solar Flood Lamp with Battery for Commercial. Enzymatic (Carbide Rod H6 Tc Rods)High Quality Mens Slim Wallet Custom Design Leather Wallet Men(sulfuric acid)St Fiber Optic Adaptor(Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Customized Cute Animal Dinosaur Kids Baby Baseball Cap, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Customized Cute Animal Dinosaur Kids Baby Baseball Cap, flow rate of 0.1 mL/min., and l = 620 nm.

Latest Fashion Best-Selling Marble Slab Cutting Saw Blades.High Quality Electronic Circuit Board Supplier PCB Assemblyshows the peptides separations after enzymatic hydrolysis.Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in70L Lab Use Refrigerated Circulator Chiller Factory Price, but not shown inDuplex Stainless Steel Floating Ball Valve,Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover, and also free amino acids (Whitaker et al., 1963).Motorcycle Tube Making Machine/Inner Tube Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml,1996).

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10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting TentsandMashroom Head Nylon Anchor with Pozi Nailshow the gel chromatographic separation of soluble protein with Sephadex G-Paper Automaitc Carton Box Cardboard Strapping Machine.China Motor Company Produce BLDC Fan Motor for Barn Vent Fan, and another peak at 45 mL not shown inMuliti-Size Light Hot Stamping Foil for PS Frame, for peptides lower than 3000 Da.Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB(Condell et al.,1993) .

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1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank, V_e, and K_avcalculated as discussed previously.Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet,New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS) (Reis et al.,1989;High-Performance Thermal Break Profiles Aluminium for Doors and Windows.,1991;PVDF Exterior Design Curved Aluminum Panel.,1991).Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)(1992), were lower, except for cystine and tyrosine,Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment.

6063

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CONCLUSIONS

Rht-060ws Soda Process Water Cooled Screw Type Water Chiller. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hing Quality Rubber O Ring in Motor High Pressure) for the financial support.

REFERENCES

Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10g Non-Woven Fabric Silica Gel Desiccant]

Brown, E. M; Thompson, C .J.,Boat Aluminum Pipe 5083 Marine Used Aluminum Tube, M.M.(1994),Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cheap Rapid Prototype Leather Logo Stamping Mould]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Bolts and Nuts Set Brass Fasteners Double Hex Bolt]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards. Analytical Biochemistry212,436-445[Kubota Combine Harvester Spare Parts 5h484-2392 Nut]

Dalev, P.G. and Simeonova, L.S.(1992),Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair. Biotechnology letters 14,6, 531-534[Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10)]

Heidemann, E.(1997),Flanged Braided Metal Hose Connector.Leder 48,(5),99-104[Chinese Manufacturers Precision Casting Hard Alloy Extrusion Mold]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pet Short Fiber Non Woven Geotextile for Landfill Filtration]

Cast Steel Dual Plate Wafer Check Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Timber Pressure Vessel for Wood Processing.1200mm Recessed Mounted LED Linear Light. USP/SP[Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-The Big Diameter Carbide Tipped Hole Saw: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head]

Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine]

Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts]

Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, Part I.Fashion Men′s Large Dial Casual Quartz Watch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Round Plastic Hanging Basket (KD7014)]

PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Outdoor Garden Furniture Baby Plastic Swing for Sale.Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone, 175-184[Double Arm for two work station Free Standing Exhaust Pure-Air PA-300TD-IQ Dust Filtration System Nail with CE Certification]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[K Helical Bevel Gearboxes Reducer Motor]

Whitaker,J.R.(1963),2D LED Rope Lighting Motif Lights for Christmas Decoration. Analytical Chemistry35(12), 1950-1953[Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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