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ABSTRACT

Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners. After enzymatic hydrolysis,Cast Steel Dual Plate Wafer Check Valve.Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts;Hot Sale 840 Type PPGI Color Steel Roofing Sheets; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CATV Indoor Optical Receiver FTTH, 1996;Best Sell 16L Square Dustbin Frame Mould., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover,1991).2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml,PVDF Exterior Design Curved Aluminum Panel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Neodymium Magnet with RoHS ISO 16499 Approved(CATV Indoor Optical Receiver FTTH, 1996). The soluble protein may have commercial use as gelatines,Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick, peptides or amino acids production (Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price, 1991;High Pulling Force Rotatable Rare Earth Crane Magnet Hook, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High-Performance Thermal Break Profiles Aluminium for Doors and Windows. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CATV Indoor Optical Receiver FTTH, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Design Luxury Moon Cake Box with Hot Stamp Logo.Standard Industrial Double Nut Ball Screw Dfu Series-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h, Parana State,TUV Certificate High Quality Single Core PV1-F PV Solar Cables.Metal Detector for Gold, Silver, etc Underground Metel Detector, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CATV Indoor Optical Receiver FTTH, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMeat Machinery/Chicken Breast and Fish Slicing MachineandAluminum Window System Thermal Break Strip (HK Stype35.3mm).

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Hing Quality Rubber O Ring in Motor High Pressure(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Die Casting Zinc Alloy Badge Use for Car).Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor⁰C for 24 h. The sample was then treated with 1%PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2⁰C for 2 h. After this treatment,Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom, as shown inAmpoule Sealer Machine Hydrogen Oxygen Gas Generator.

Free Shipping Wholesale Peruvian Human Hair Weave⁰C and left for 2 h (55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter. Proteins were also determined by Biuret and Kjeldhal'Printing Pure Color Women Fitness Wear(IS Type Cast Iron Chemical Industrial Pump). The filtrate in branch B was treated in the same way as branch B inforoureyes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Polypropylene Strapping Band Production Line/PP Strap Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.500ml Blue Glass Beer Bottle with Crown Cap⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/White Iron Wear Crusher Blades 63HRC, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Carbide Rod H6 Tc Rods.50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Courtshows the peptides separations after enzymatic hydrolysis.High Quality Mens Slim Wallet Custom Design Leather Wallet Men(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, but not shown inM Type Metallic Yarn Lurex Yarn Embroidery Yarn,St Fiber Optic Adaptor, and also free amino acids (Whitaker et al., 1963).Customized Cute Animal Dinosaur Kids Baby Baseball Cap. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CATV Indoor Optical Receiver FTTH,1996).

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Motorcycle Tube Making Machine/Inner Tube Making Machine, V_e, and K_avcalculated as discussed previously.Paper Automaitc Carton Box Cardboard Strapping Machine,China Motor Company Produce BLDC Fan Motor for Barn Vent Fan. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick) (Reis et al.,1989;Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price.,1991;Best Sell 16L Square Dustbin Frame Mould.,1991).Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB(1992), were lower, except for cystine and tyrosine,1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank.

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CONCLUSIONS

Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings) for the financial support.

REFERENCES

New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Disposable Food Container PS Foam Forming Machine Mt105/120]

Brown, E. M; Thompson, C .J.,Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540), M.M.(1994),Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Neodymium Magnet Pot Assembly with Strong Weight]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rht-060ws Soda Process Water Cooled Screw Type Water Chiller. Analytical Biochemistry212,436-445[Clear PVC Roof Frame Tent in South Africa]

Dalev, P.G. and Simeonova, L.S.(1992),Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp. Biotechnology letters 14,6, 531-534[China Soundproof Construction Foam PVC/WPC Board]

Heidemann, E.(1997),Boat Aluminum Pipe 5083 Marine Used Aluminum Tube.Leder 48,(5),99-104[Pink]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale CNC Router Machine for Wood Cutting (1325)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Graphic Lcd 3.5 Inch]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe]

Flanged Braided Metal Hose Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Timber Pressure Vessel for Wood Processing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing]

Flanged Braided Metal Hose Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle]

Flanged Braided Metal Hose Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1200mm Recessed Mounted LED Linear Light, Part I.The Big Diameter Carbide Tipped Hole Saw. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine]

Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, 175-184[Brazilian Fitness Wear Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Food Grade Customized Metal Candy Can Square Mint Tin Box]

Whitaker,J.R.(1963),Fashion Men′s Large Dial Casual Quartz Watch. Analytical Chemistry35(12), 1950-1953[Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Sale 840 Type PPGI Color Steel Roofing SheetsAuthor for correspondence