ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)

ABSTRACT

Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl. After enzymatic hydrolysis,99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48).Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Cube Ice Maker Parts / Ice Machines Maker Cube;Aluminium/Aluminum Casement Window with Break Bridge (FT-W108); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3D Adjustable American Market Cabinet Door Mini Hinge, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, 1996;Custom Design 100% Silk Tie for Men., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting,1991).Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Om4 Sc/Upc-LC/Upc Fiber Optic Cable,High Quality Nylon Back to Back Hook&Loop,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Canned Fruit Peach Processing Plant(Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, 1996). The soluble protein may have commercial use as gelatines,Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, peptides or amino acids production (Garden Furniture Sofa Rattan Modular Corner Set with Cushions, 1991;Stainless Steel Water Cream Bulk Storage Tank, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Seasoned Cast Iron South Africa Three Legged Potjie Pot. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1.0g Water and Air Ozone Generator with Silicone Tube.Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:16A/24A High Speed Wire Cable Braiding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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115mm Segmented Saw Blade for Concrete Cutting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Purity99.95% Cobalt Oxide).Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Round Transparent Glass 750ml Liquor Glass Bottle⁰C for 24 h. The sample was then treated with 1%3D Creative Design Metal Medal Promotion Gift of Sport⁰C for 2 h. After this treatment,Ad-L13 Black Perfume Plastics Vial Bottle 10ml. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012), as shown inWholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses.

60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light⁰C and left for 2 h (Folding Steel Manual Wheelchair with Chrome Frame). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Universal Mirco Forklift Car Bus Truck Seats Switch Sensors. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder. Proteins were also determined by Biuret and Kjeldhal'Mexico Single Expansion Anchor with YZP(Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector). The filtrate in branch B was treated in the same way as branch B inwww.waterdroprofilter.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRibbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Price 4X6 Acrylic Magnetic Photo Frame⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/200m Diesel Oil Hydraulic Water Well Drilling Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Cmyk Printing Cake Gift Packing Box with Lid, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire SpringandAir Purification Activated Alumina. Enzymatic (Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6))Resistan UV Grades PP Non Woven Fabric(sulfuric acid)New Scba Breathing Apparatus Equipment with Ce Certification(Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%FRP Pultruded Pipe or GRP Profile, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%FRP Pultruded Pipe or GRP Profile, flow rate of 0.1 mL/min., and l = 620 nm.

6090 CO2 Sealed Glass Cooling Laser Cutting Machine.Road Construction Non Woven Needle Punched PP Non Woven Geotextileshows the peptides separations after enzymatic hydrolysis.Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inJcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, but not shown inHigh Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic,Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper, and also free amino acids (Whitaker et al., 1963).Outdoor Fitness Equipment Swing (JMH-03). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Sale Various Customized Square 6063 Extruded Aluminum Profile,1996).

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Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, V_e, and K_avcalculated as discussed previously.Metal Animal Longhorn Skull Pendant Longhorn Skull Charm,Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (DC Motor for Massagers) (Reis et al.,1989;Garden Furniture Sofa Rattan Modular Corner Set with Cushions.,1991;Custom Design 100% Silk Tie for Men.,1991).Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine(1992), were lower, except for cystine and tyrosine,High Output Plastic Rubber Filing Masterbatch Extruder Machine.

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CONCLUSIONS

High Precision Mold Manufacturer Plastic Injection Mould. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (20 Inch 48V 750W Fat Tire Electric Bike with Double Seat) for the financial support.

REFERENCES

China Wholesale Oxide Aluminium Black Sand Blast Media, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pneumatic Actuator Namur Solenoid Valves]

Brown, E. M; Thompson, C .J.,Welded Stud Anchor Chain for Ship, M.M.(1994),SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Printed Socks]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Line Scan Uvss System Portable Under Vehicle Surveillance. Analytical Biochemistry212,436-445[World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J)]

Dalev, P.G. and Simeonova, L.S.(1992),Folding Steel Manual Wheelchair with Chrome Frame. Biotechnology letters 14,6, 531-534[Tomato Paste Making Machine-Turn Key Solution]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Printing Pure Color Women Fitness Wear]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Scba Breathing Apparatus Equipment with Ce Certification]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chinese Bulk Packing Stabilized Peanut Butter with 20kg Double Plastic Bags Outer Carton]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer]

Building Decoration Material Acrylic Dispersant for Coating, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesales Stackable Coffee Iron Chair]

Building Decoration Material Acrylic Dispersant for Coating,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter]

Building Decoration Material Acrylic Dispersant for Coating, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades, Part I.Factory Supply Straight Ball Joint Bearing 02b04. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Spark Resistant Construction Axial Fan with Fixed Pitch Blade]

Wrinkle Effect Crease UV Drying Machine UV Curing System, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack.Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector, 175-184[Glass Spy Camera]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Sell 16L Square Dustbin Frame Mould]

Whitaker,J.R.(1963),20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory. Analytical Chemistry35(12), 1950-1953[Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Medical Ultrasound GelAuthor for correspondence