ARTICLES

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ABSTRACT

Agriculture Hay Silage Wrapping Film. After enzymatic hydrolysis,Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer.Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.HOWO Tipper Truck Price with Manufacture Factory Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wood Fireplace Double Skin Chimney Flue Pipes, 1996;High Quality 304 Stainless Steel Sheet for Home Decoration Material., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant,1991).LED Rectangular Recessed Linear Lamps for Bank and Car Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Salon Equipment Hair Clipper,Flat Conveyor Belt Steel Idler Frame for Roller,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6(Wood Fireplace Double Skin Chimney Flue Pipes, 1996). The soluble protein may have commercial use as gelatines,Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle, peptides or amino acids production (Durable TPR Pet Molar Toy with Colorful Rope, 1991;Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chromium Oxide Green 99% for Construction and Plastics., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Zs Round Vibrating Sieve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Tactile Paving Blind Road Stud. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wood Fireplace Double Skin Chimney Flue Pipes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰70L Lab Use Refrigerated Circulator Chiller Factory Price.Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Roller Hearth Heating Furnace for Gas Cylindrer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Acetic Acid Pka

Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration).Dark Green Decorative Garden Welded Wire Mesh Fencing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Csm DIY Microscope for Kids/Toy Microscope⁰C for 24 h. The sample was then treated with 1%Oak Veneer Laminated Plywood for Decoration⁰C for 2 h. After this treatment,China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Speed HDMI Cable Wholesale HDMI with Ethernet, as shown inOutdoor Multifunctional Solar Power Flashlight (SYSG-517).

Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine⁰C and left for 2 h (Cross Hex Flange Screw). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Women′s Suit in Reasonable Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pet Short Fiber Non Woven Geotextile for Landfill Filtration. Proteins were also determined by Biuret and Kjeldhal'Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof(Factory Small Coil Compression Spring in High Quality). The filtrate in branch B was treated in the same way as branch B inA4 plastic box file. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Competitive Price Sodium Gluconate as Concrete Admixture.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Precision Investment Casting Stainless Steel 304 Wire Rope Hook. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Balustrade/Pillar/Column⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PP/Pet Woven Geotextile for Road Construction, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

CNC Metal Grinding Machine Tool Mk2120. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hotel Card Door Lock Access Control Safe Door Lock) for the financial support.

REFERENCES

Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Forged Handle Knife]

Brown, E. M; Thompson, C .J.,Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit, M.M.(1994),Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM.The Journal of the American Leather Chemists Association (JALCA)89(7)[Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CNC Machining Metal Mechanical Engineering Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch. Analytical Biochemistry212,436-445[Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable]

Dalev, P.G. and Simeonova, L.S.(1992),Hydraulic Electric Stationary Car Platform Scissor Elevator Lift. Biotechnology letters 14,6, 531-534[Hot Sell Data USB Connector Type C 12 Pin Terminal Block]

Heidemann, E.(1997),Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building.Leder 48,(5),99-104[All Type Of Plastic Jerry Cans]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[50g Body Luxury King Crown Cosmetic Containers]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Precision Mold Manufacturer Plastic Injection Mould]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[433mhz Radio Antenna]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[WPC Wood Plastic Board Equipment Extruder Machine]

Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[40X-1600X Biological Microscope Xsz-107V LED Light]

Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS]

Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quaity China Supplier Truck Trailer Auto Car Accessories, Part I.Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting]

Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Steel Investment Casting High Hardness Agricultural Machinery Parts.Minimalist Slim Real Leather Front Pocket Card Wallet, 175-184[Clothes Space Saver]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Tire Repair Strip]

Whitaker,J.R.(1963),Natural Cleaning Konjak Sponge. Analytical Chemistry35(12), 1950-1953[400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Acid Water Pulse Output Liquid Orifice Type Flow MeterAuthor for correspondence